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Role of syntaxin in mouse pancreatic beta cells

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Summary

The role of syntaxin 1, a protein involved in the docking of synaptic vesicles at presynaptic active zones, has been investigated in pancreatic islet cells. Using two different monoclonal antibodies we have shown that syntaxin 1 is present in the pancreatic islet cell microsomal fraction. Furthermore, functional experiments demonstrate that anti-syntaxin antibodies inhibit Ca2+-dependent insulin secretion in permeabilized islet cells. These data indicate that syntaxin 1 is present in the pancreatic beta cell and it is likely to play a functional role in the exocytosis of secretory granules.

Abbreviations

[Ca2+]i :

Intracellular free calcium concentration

PMSF:

phenyl methyl sulphonyl fluoride

PVDF:

polyvinylidene difluoride

SNAP:

synaptosomal associated protein

SNARE:

SNAP receptor

VAMP:

vesicle-associated membrane protein

mAb:

monoclonal antibody]

clone HPC-1:

anti-syntaxin monoclonal antibody

References

  1. 1.

    Bennet MK, Scheller RH (1994) A molecular description of synaptic vesicle membrane trafficking. Ann Rev Bio-chem 63: 63–100

  2. 2.

    Jacobsson G, Bean AJ, Scheller RH et al. (1994) Identification of synaptic proteins and their isoform mRNAs in compartments of pancreatic endocrine cells. Proc Natl Acad Sci USA 91: 12487–12491

  3. 3.

    Thomas-Reetz A, Hell JW, During MJ, Walch-Solimena C, Jahn R, De Camilli P (1993) A γ-aminobutyric acid transporter driven by a proton pump is present in synaptic-like microvesicles of pancreatic β-cells. Proc Natl Acad Sci USA 87: 5692–5696

  4. 4.

    Cerezo JR, Jimenez F, Moya F (1995) Characterization and gene cloning of Drosophila syntaxin 1 (Dsynt 1): the fruit fly homologue of rat syntaxin 1. Mol Brain Res 29: 249–252

  5. 5.

    Valdeolmillos M, Nadal A, Contreras D, Soria B (1992) The relationship between glucose-induced K+ ATP channel closure and the rise in [Ca2+]i in single mouse pancreatic β-cells. J Physiol 455: 173–186

  6. 6.

    Cotman CW (1974) Isolation of synaptosomal and synaptic plasma membrane fractions. Methods Enzymol 31: 445–452

  7. 7.

    Bennet MK, García-Arrarás JE, Elferink LA et al. (1993) The syntaxin family of vesicular transport receptors. Cell 74: 863–873

  8. 8.

    Inoue A, Akagawa K (1993) Neuron specific expression of a membrane protein, HPC-1: tissue distribution, and cellular and subcellular localization of immunoreactivity and mRNA. Mol Brain Res 19: 121–128

  9. 9.

    Gutierrez LM, Quintanar JL, Viniegra S, Salinas E, Moya F, Reig JA (1995) Anti-syntaxin antibodies inhibit calcium-dependent catecholamine secretion from permeabilized chromaffin cells. Biochem Biophys Res Commun 206: 1–7

  10. 10.

    Ämmälä C, Elliason L, Bokvist K, Larsson O, Ashcroft FM, Rorsman P (1993) Exocytosis elicited by action potentials and voltage-clamp calcium currents in individual mouse pancreatic B-cells. J Physiol 472: 665–688

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Author information

Correspondence to F. Martín or F. Moya or L. M. Gutierrez or J. A. Reig or B. Soria.

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Martín, F., Moya, F., Gutierrez, L.M. et al. Role of syntaxin in mouse pancreatic beta cells. Diabetologia 38, 860–863 (1995). https://doi.org/10.1007/s001250050364

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Key words

  • Exocytosis
  • syntaxin
  • pancreatic beta cell
  • secretion
  • SNAP receptor