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Role of syntaxin in mouse pancreatic beta cells

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The role of syntaxin 1, a protein involved in the docking of synaptic vesicles at presynaptic active zones, has been investigated in pancreatic islet cells. Using two different monoclonal antibodies we have shown that syntaxin 1 is present in the pancreatic islet cell microsomal fraction. Furthermore, functional experiments demonstrate that anti-syntaxin antibodies inhibit Ca2+-dependent insulin secretion in permeabilized islet cells. These data indicate that syntaxin 1 is present in the pancreatic beta cell and it is likely to play a functional role in the exocytosis of secretory granules.


[Ca2+]i :

Intracellular free calcium concentration


phenyl methyl sulphonyl fluoride


polyvinylidene difluoride


synaptosomal associated protein


SNAP receptor


vesicle-associated membrane protein


monoclonal antibody]

clone HPC-1:

anti-syntaxin monoclonal antibody


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Correspondence to F. Martín or F. Moya or L. M. Gutierrez or J. A. Reig or B. Soria.

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Martín, F., Moya, F., Gutierrez, L.M. et al. Role of syntaxin in mouse pancreatic beta cells. Diabetologia 38, 860–863 (1995). https://doi.org/10.1007/s001250050364

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Key words

  • Exocytosis
  • syntaxin
  • pancreatic beta cell
  • secretion
  • SNAP receptor