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The impact of ethnicity on glucose regulation and the metabolic syndrome following gestational diabetes

Abstract

Aims/hypothesis

We assessed the impact of ethnic origin on metabolism in women following gestational diabetes mellitus (GDM).

Materials and methods

Glucose regulation and other features of the metabolic syndrome were studied at 20.0 (18.2–22.1) months (geometric mean [95% CI]) post-partum in women with previous GDM (185 European, 103 Asian-Indian, 80 African-Caribbean). They were compared with the same features in 482 normal control subjects who had normal glucose regulation during and following pregnancy.

Results

Impaired glucose regulation or diabetes by WHO criteria were present in 37% of women with previous GDM (diabetes in 17%), especially in those of African-Caribbean and Asian-Indian origin (50 and 44%, respectively vs 28% in European, p=0.009). BMI, waist circumference, diastolic blood pressure, fasting triglyceride and insulin levels, and insulin resistance by homeostatic model assessment (HOMA), were increased following GDM (p<0.001 for all, vs control subjects). Where glucose regulation was normal following GDM, basal insulin secretion (by HOMA) was high (p<0.001 vs control subjects). Irrespective of glucose regulation in pregnancy, Asian-Indian origin was associated with high triglyceride and low HDL cholesterol levels, and African-Caribbean with increased waist circumference, blood pressure, and insulin levels, together with insulin resistance and low triglyceride concentrations. Nonetheless, the GDM-associated features were consistent within each ethnic group. The metabolic syndrome by International Diabetes Federation criteria was present in 37% of women with previous GDM, especially in non-Europeans (Asian-Indian 49%, African-Caribbean 43%, European 28%, p=0.001), and in 10% of controls.

Conclusions/interpretation

Following GDM, abnormal glucose regulation and the metabolic syndrome are common, especially in non-European women, indicating a need for diabetes and cardiovascular disease prevention strategies.

Introduction

Subjects with the metabolic syndrome are at increased risk of cardiovascular disease and diabetes [1]. Gestational diabetes mellitus (GDM) is defined as hyperglycaemia first recognised during pregnancy, and although glucose regulation usually returns to normal following delivery, features of the metabolic syndrome may be present and glucose tolerance often deteriorates with time [2]. Reports on metabolism following GDM have shown variable results [3], and we hypothesised that this variability reflected ethnic heterogeneity in different studies, small subject numbers and different times between the index pregnancy and study. We therefore examined glucose regulation and metabolism in a large group of women from three ethnic groups, within 1 to 3 years of GDM pregnancy. They were compared with women who had normal glucose regulation during and after pregnancy.

Subjects, materials and methods

Subjects

Between 1997 and 1999 we retrospectively recruited 368 women with previous GDM (pGDM) from West London databases. Recruitment was at 20.0 months (geometric mean, 95% CI 18.2–22.1 months) following delivery. Entry was based on WHO criteria for glucose intolerance during pregnancy [4], and on the women being of European (n=185), Asian-Indian (India, Pakistan, Bangladesh or Sri Lanka, n=103) or African-Caribbean (n=80) ethnicity.

As controls we recruited 482 normal women at 21.6 (20.0–23.4) months after the index pregnancy. Their entry criteria were: normal glucose levels throughout the index (and other) pregnancies, normal fasting glucose and HbA1c at recruitment and European (n=288), Asian-Indian (n=99) or African-Caribbean (n=95) ethnicity as specified above.

Ethical approval was from the UK Multicentre Research Ethics Committee and local ethical committees and informed consent was obtained.

Tests performed

Anthropometry was assessed and resting BP, fasting lipids and specific insulin were measured. Biochemical assays were performed as described previously [5]. Of the women with pGDM, 227 out of 368 (62%) underwent a 75-g OGTT and the remainder (including 37 with known type 2 diabetes) had measurement of fasting glucose and HbA1c. The pGDM group was subdivided into two: pGDM with normal glucose regulation post-partum (NG) and pGDM with abnormal glucose regulation post-partum (AG) [4]. Beta cell function (%B) and insulin sensitivity (%S) were derived using homeostatic model assessment (HOMA) [6].

The metabolic syndrome was defined by International Diabetes Federation (IDF) guidelines [7].

Statistical analysis

Data are presented as means (95% CI) or, where log transformed, as geometric means (95% CI). The prevalence of abnormal glucose regulation by ethnic group was compared by the χ 2 test.

To determine the relationship between individual metabolic syndrome features and history of GDM, multiple linear regression models were used, with adjustment for age, parity, time between delivery and study and ethnicity. Regression models were used to study the effect of ethnicity, adjusting also for glucose regulation. For outcomes that did not require log transformation, results are reported as regression coefficients. When logarithmic transformation was necessary, regression coefficients are reported as percentage changes. For ethnic comparisons, Europeans were the reference population. In view of the multiple comparisons, and in order to concentrate on differences of potential clinical importance, we adopted a stringent significance threshold (p≤0.001).

Results

Glucose regulation and the metabolic syndrome following GDM

The age of the pGDM group (geometric mean [95% CI] 35.7 [35.2–36.2] years) was similar to controls (34.8 [34.3–35.3] years), as was the time between study and delivery. pGDM women were of higher parity (2.1 [2.0–2.2] vs 1.8 [1.7–1.9]; p<0.001).

Of the 368 women with pGDM, 137 (37%) at the time of study had abnormal glucose levels (diabetes [n=62, including 37 with known diabetes], impaired glucose tolerance [n=51], or impaired fasting glycaemia [n=24]). Normal fasting glycaemia was as common among the 227 women with pGDM who had an OGTT (87%) as in the 104 who did not (81%).

There was no difference in age, or in time between pregnancy and study, between the pGDM subgroups (i.e. pGDM-NG vs pGDM-AG), although the latter were of higher parity (2.4 [2.1–2.6] vs 1.9 [1.8–2.1]; p<0.001).

IDF-defined metabolic syndrome was observed in 37% of women with pGDM and 10% of controls. Women with pGDM had higher BMI and waist circumference, systolic and diastolic BP and fasting triglyceride than controls (Table 1). The differences in total and HDL cholesterol did not achieve the required significance level. Fasting glucose was increased, insulin levels were almost double and HOMA %S almost half those in controls (both p<0.001). The two groups did not differ significantly in HOMA %B (p=0.25).

Table 1 The features of the metabolic syndrome among women with a history of gestational diabetes mellitus (GDM)

Differences between pGDM-NG and control women were similar to those in the entire pGDM group (except systolic BP). The pGDM-NG women had elevated HOMA %B.

Women with pGDM-AG had higher BMI and waist circumference than pGDM-NG women. These groups differed in beta cell function (HOMA %B) rather than insulin sensitivity (HOMA %S).

Additional adjustment for BMI had little impact. Apart from systolic and diastolic BP when comparing pGDM subjects with controls, and diastolic BP when comparing the pGDM-NG with controls, other statistically significant differences persisted. Exclusion of the 37 women with known diabetes were excluded, statistical significance in the comparison between European and African Carribean women was lost for insulin (p=0.005) but other ethnic differences persisted.

Ethnic influences on metabolism

Compared with Europeans, Asian-Indian women were slightly younger and had higher triglyceride and lower HDL cholesterol; African-Caribbean women had higher BMI, waist circumference, systolic and diastolic BP and lower fasting triglyceride (Table 2). Insulin was higher and HOMA %S lower. When women with known diabetes were excluded, statistical significance in the comparison between European and African-Caribbean women was lost for insulin (p=0.005), but other ethnic differences persisted.

Table 2 Consequences of ethnic origin on the features of the metabolic syndrome and on the sequelae of previous gestational diabetes mellitus (pGDM)

Among the 137 pGDM women with abnormal glucose regulation, 52 were Europeans (28%), 45 Asian-Indians (44%), and 40 African-Caribbeans (50%). The prevalence of abnormal glucose values of any degree in both Asian-Indian and African-Caribbean pGDM women was higher than in Europeans (χ 2=14.01, p=0.009). The prevalence of type 2 diabetes following GDM was also higher in both Asian-Indian (26%) and African-Caribbean women (22%) than in European women (10%) (χ 2=13.95, p=0.001).

Of the 136 pGDM women with IDF-defined metabolic syndrome, 49% were of Asian-Indian origin, 43% were African-Caribbean and 28% were European (χ 2=13.4, p=0.001).

To establish whether the relationship between metabolic syndrome features and glucose regulation status (previous and current) was similar in the different ethnic groups, we sought statistical interactions between ethnicity and glycaemic status. There were no significant interactions between ethnicity and pGDM/control or pGDM-NG/pGDM-AG status. Borderline interactions between pGDM/control status and ethnicity were observed for fasting insulin (p=0.0012), glucose (p=0.008) and HOMA %S (p=0.0014). pGDM-associated relative differences in BMI, waist circumference, BP and triglyceride were consistent across the ethnic groups, even though absolute values varied by ethnic origin. On further analysis of the borderline interactions, fasting insulin in the Asian-Indian women with pGDM was higher than expected from the main effects attributable to ethnicity or pGDM status (p<0.001), and HOMA %S was lower (p<0.001).

Discussion

This study of 850 women with young children involved certain compromises, e.g. only fasting samples, rather than an OGTT, were obtained in 32% of the pGDM women, and GDM screening policies differed between centres. However, all pGDM patients had fulfilled WHO criteria during pregnancy, and the women who had an OGTT subsequently had similar fasting glucose levels to those who did not.

The high prevalence of abnormal glucose regulation following GDM (37%) is similar to other studies of equivalent follow-up [2]. Metabolic syndrome features were equally common. They have been observed in other situations of high diabetes risk, e.g. polycystic ovary syndrome, and they precede diabetes in prospective studies [8].

Women with previous GDM and abnormal glucose homeostasis had reduced beta cell function, but those with normoglycaemia post-partum had high basal insulin secretion (by HOMA %B). Thus although insulin resistance is ubiquitous, hyperglycaemia post-partum reflects the inability to mount an adequate insulin response. Nonetheless, in a subset of women with normoglycaemia post-partum, we have detected subtle beta cell deficiency using an i.v. glucose challenge (5).

Asian-Indian women had an adverse lipid profile compared with Europeans, irrespectively of glucose regulation. They were not significantly insulin resistant, but those with pGDM had higher than expected insulin levels. These ethnic differences are unlikely to reflect merely the younger age of the Asian-Indian women as the difference was small (2–3 years) and the statistical analysis was adjusted for age. African-Caribbean women were heavier than Europeans and had increased waist circumference. They were insulin resistant and had higher BP and lower triglyceride levels. These ethnic differences are similar to previous reports, mostly from studies in men [9].

Within each ethnic group, features associated with pGDM were consistent. WHO-defined abnormal glucose regulation following GDM was most common in non-European women, as in some previous studies [10]. IDF-defined metabolic syndrome was also most common in non-Europeans. These features may have preceded pregnancy and suggest that non-European women with pGDM are at the highest risk of developing cardiovascular disease and diabetes. The metabolic syndrome was present in a high proportion (43%) of the African-Caribbean women even though the IDF definition takes account of ethnic differences in waist circumference. Given that diabetes and cardiovascular disease may be prevented by lifestyle modification and/or drug therapy, prevention strategies may be especially important for non-European women.

Abbreviations

AG:

abnormal glucose regulation post-partum

%B:

beta cell function

BP:

blood pressure

%S:

insulin sensitivity

GDM:

gestational diabetes mellitus

HOMA:

homeostatic model assessment

IDF:

International Diabetes Federation

NG:

normal glucose regulation post-partum

pGDM:

previous gestational diabetes mellitus

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Acknowledgements

This study was funded by the UK Medical Research Council and Diabetes UK. J. A. R. Jeffs and I. F. Godsland were supported by the Heart Disease and Diabetes Research Trust.

Author information

Correspondence to D. G. Johnston.

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Kousta, E., Efstathiadou, Z., Lawrence, N.J. et al. The impact of ethnicity on glucose regulation and the metabolic syndrome following gestational diabetes. Diabetologia 49, 36–40 (2006). https://doi.org/10.1007/s00125-005-0058-6

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Keywords

  • Ethnicity
  • Gestational diabetes
  • Glucose tolerance
  • Metabolic syndrome
  • Type 2 diabetes