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Isolation, characterization and chromosomal location of a novel zinc-finger protein gene that is down-regulated by salt stress

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Abstract 

mRNA transcripts from salt-stressed and unstressed rice seedlings were detected by differential display to show alterations in abundance. One transcript, designated OsZFP1, was found to be significantly down-regulated by salt stress. OsZFP1 encodes a protein of 145 amino acids with three putative Cys2/Cys2-type zinc-finger domains. A homology search of GenBank databases showed that OsZFP1 is homologous to the rat and human ZIS(zinc-finger, splicing) proteins and the human nucleopore complex protein Nup358 in the zinc- finger domains. Genomic Southern analysis indicated that the OsZFP1 gene was present as a single-copy sequence in the rice genome. Restriction fragment length polymorphism mapping assigned the OsZFP1 gene to the distal position of chromosome 6. A RT-PCR assay showed that the OsZFP1 transcripts were more abundant in rice shoots than in the roots. Decreases in the level of OsZFP1 transcripts were detected in the shoots after 6 h of salt stress and in the roots after 3 h of salt stress. In addition, the expression of OsZFP1 in rice shoots was significantly repressed by exogenous application of abscisic acid. The results suggest that OsZFP1 represents a novel type of zinc-finger protein gene in plants and that it is implicated in the responses of rice plants to salt stress.

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Received: 29 March 2000 / Accepted. 30 April 2000

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Li, Z., Chen, S. Isolation, characterization and chromosomal location of a novel zinc-finger protein gene that is down-regulated by salt stress. Theor Appl Genet 102, 363–368 (2001). https://doi.org/10.1007/s001220051654

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  • Keywords Differential display
  • Down-regulation
  • Rice (Oryza sativa L.)
  • Salt stress
  • Zinc-finger protein