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Molecular mapping of major QTL conferring resistance to orange wheat blossom midge (Sitodiplosis mosellana) in Chinese wheat varieties with selective populations

  • Lijing Zhang
  • Miaomiao Geng
  • Zhe Zhang
  • Yue Zhang
  • Guijun Yan
  • Shumin Wen
  • Guiru LiuEmail author
  • Ruihui WangEmail author
Original Article
  • 45 Downloads

Abstract

Key message

Two novel midge resistance QTL were mapped to a 4.9-Mb interval on chromosome arm 4AL based on the genetic maps constructed with SNP markers.

Abstract

Orange wheat blossom midge (OWBM) is a devastating insect pest affecting wheat production. In order to detect OWBM resistance genes and quantitative trait loci (QTL) for wheat breeding, two recombinant inbred line (RIL) populations were established and used for molecular mapping. A total of seven QTL were detected on chromosomes 2D, 4A, 4D and 7D, respectively, of which positive alleles were all from the resistant parents except for the QTL on 7D. Two stable QTL (QSm.hbau-4A.2-1 and QSm.hbau-4A.2-2) were detected in both populations with the LOD scores ranging from 5.58 to 29.22 under all three environments, and they explained a combined phenotypic variation of 24.4–44.8%. These two novel QTL were mapped to a 4.9-Mb physical interval. The single-nucleotide polymorphism (SNP) markers AX-109543456, AX-108942696 and AX-110928325 were closely linked to the QTL and could be used for marker-assisted selection (MAS) for OWBM resistance in wheat breeding programs.

Abbreviations

ANOVA

Analysis of variance

EST

Expressed sequence tag

ICIM

Inclusive composite interval mapping

KASP

Kompetitive allele specific PCR

LOD

Logarithm of odds

MAS

Marker-assisted selection

NIL

Near-isogenic line

QTL

Quantitative trait loci

RIL

Recombinant inbred line

SNP

Single-nucleotide polymorphism

SSR

Simple sequence repeat

OWBM

Orange wheat blossom midge

Notes

Acknowledgements

We are grateful to Prof. Xinming Yang (Institute of Crop Sciences, Chinese Academy of Agricultural Sciences) for supplying the pedigree of some wheat varieties, Dr. Guangyao Zhao (Institute of Crop Sciences, Chinese Academy of Agricultural Sciences) for his advice in comparative genomic analysis, Prof. Zhengang Qu (Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences) for his advice in the identification of OWBM resistance, Dr. Yanru Cui (Hebei Agricultural University) for her help in selective population analysis and her careful review for this paper.

Author Contribution statement

LZ and RW designed and conducted the study. MG, GY, SW and GL provided advice to the authors. SW, GL and RW performed RIL population construction. GL and RW performed OWBM invasion treatment and analyzed resistance index. LZ and ZZ performed experimental material collection for SNP genotyping. LZ, ZZ and YZ performed resistance evaluation.

Funding

This work was supported by National Natural Science Foundation of China (31371617).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

All research conducted in relation to this publication is in compliance with ethical standards. The authors declare that this study was performed and reported in accordance with the ethical standards of scientific conduct.

Supplementary material

122_2019_3480_MOESM1_ESM.xlsx (1.1 mb)
Supplementary material 1 (XLSX 1120 kb)
122_2019_3480_MOESM2_ESM.pdf (12.6 mb)
Supplementary material 2 (PDF 12899 kb)
122_2019_3480_MOESM3_ESM.pdf (204 kb)
Supplementary material 3 (PDF 203 kb)

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Copyright information

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Authors and Affiliations

  1. 1.North China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of AgronomyHebei Agricultural UniversityBaodingPeople’s Republic of China
  2. 2.School of Agriculture and Environment, Faculty of Science, and the Institute of AgricultureThe University of Western AustraliaPerthAustralia

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