Fine-mapping of a major QTL (Fwr1) for fusarium wilt resistance in radish
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A major radish QTL (Fwr1) for fusarium wilt resistance was fine-mapped. Sequence and expression analyses suggest that a gene encoding a serine/arginine-rich protein kinase is a candidate gene for Fwr1.
Fusarium wilt resistance locus 1 (Fwr1) is a major quantitative trait locus (QTL) mediating the resistance of radish inbred line ‘B2’ to Fusarium oxysporum, which is responsible for fusarium wilt. We previously detected Fwr1 on radish linkage group 3 (i.e., chromosome 5). In this study, a high-resolution genetic map of the Fwr1 locus was constructed by analyzing 354 recombinant F2 plants derived from a cross between ‘B2’ and ‘835’, the latter of which is susceptible to fusarium wilt. The Fwr1 QTL was fine-mapped to a 139.8-kb region between markers FM82 and FM87 in the middle part of chromosome 5. Fifteen candidate genes were predicted in this region based on a sequence comparison with the ‘WK10039’ radish reference genome. Additionally, we examined the time-course expression patterns of these predicted genes following an infection by the fusarium wilt pathogen. The ORF4 expression level was significantly higher in the resistant ‘B2’ plants than in the susceptible ‘835’ plants. The ORF4 sequence was predicted to encode a serine/arginine-rich protein kinase and includes SNPs that result in nonsynonymous mutations, which may have important functional consequences. This study reveals a novel gene responsible for fusarium wilt resistance in radish. Further analyses of this gene may elucidate the molecular mechanisms underlying the fusarium wilt resistance of plants.
This research was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries through the Golden Seed Project, which is funded by the Ministry of Agriculture, Food and Rural Affairs (Grant Numbers 213006-05-3-SBO20 and 213006-05-3-SB110).
Author contribution statement
XY and LL designed the experiment, carried out the marker development, analyzed all data, and drafted the manuscript. LL and YM were participated in phenotype evaluations, and marker survey and genotyping, candidate gene identification. SSC participated in data analysis and modification of the manuscript. SYY interpreted the data and designed the experiment. YPL provided plant materials, conceived the study, and finalized the manuscript. SRC conceived and designed the study, participated as a director, and wrote the manuscript. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors have no conflicts of interest to declare.
- Brotman Y, Normantovich M, Goldenberg Z, Zvirin Z, Kovalski I, Stovbun N, Doniger T, Bolger AM, Troadec C, Bendahmane A, Cohen R, Katzir N, Pitrat M, Dogimont C, Perl-Treves R (2013) Dual resistance of melon to Fusarium oxysporum races 0 and 2 and to Papaya ring-spot virus is controlled by a pair of head-to-head-oriented NB-LRR genes of unusual architecture. Mol Plant 6:235–238PubMedCrossRefPubMedCentralGoogle Scholar
- Brueggeman R, Druka A, Nirmala J, Cavileer T, Drader T, Rostoks N, Mirlohi A, Bennypaul H, Gill U, Kudrna D, Whitelaw C, Kilian A, Han F, Sun Y, Gill K, Steffenson B, Kleinhofs A (2008) The stem rust resistance gene Rpg5 encodes a protein with nucleotide-binding-site, leucine-rich, and protein kinase domains. Proc Natl Acad Sci 105:14970–14975PubMedCrossRefPubMedCentralGoogle Scholar
- Cao A, Xing L, Wang X, Yang X, Wang W, Sun Y, Qian C, Ni J, Chen Y, Liu D, Wang X, Chen P (2011) Serine/threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat. Proc Natl Acad Sci 108:7727–7732PubMedCrossRefPubMedCentralGoogle Scholar
- Catanzariti AM, Do HT, Bru P, de Sain M, Thatcher LF, Rep M, Jones DA (2017) The tomato I gene for Fusarium wilt resistance encodes an atypical leucine-rich repeat receptor-like protein whose function is nevertheless dependent on SOBIR 1 and SERK 3/BAK 1. Plant J 89(6):1195–1209PubMedCrossRefPubMedCentralGoogle Scholar
- Kaneko Y, Kimizukaa-Takagi C, Bang SW, Matsuzawa Y (2007) Radish. In: Kole C (ed) Genome mapping and nolecular breeding in plant, vol 5. Springer, New York, pp 141–160Google Scholar
- Kitashiba H, Li F, Hirakawa H, Kawanabe T, Zou Z, Hasegawa Y, Tonosaki K, Shirasawa S, Fukushima A, Yokoi S, Takahata Y, Kakizaki T, Ishida M, Okamoto S, Sakamoto K, Shirasawa K, Tabata S, Nishio T (2014) Draft sequences of the radish (Raphanus sativus L.) genome. DNA Res 21:481–490PubMedPubMedCentralCrossRefGoogle Scholar
- Kumar Y, Zhang L, Panigrahi P, Dholakia BB, Dewangan V, Chavan SG, Kunjir SM, Wu X, Li N, Rajmohanan PR, Kadoo NY, Giri AP, Tang H, Gupta VS (2016) Fusarium oxysporum mediates systems metabolic reprogramming of chickpea roots as revealed by a combination of proteomics and metabolomics. Plant Biotechnol J 14:1589–1603PubMedPubMedCentralCrossRefGoogle Scholar
- Marchler-Bauer A, Derbyshire MK, Gonzales NR, Lu S, Chitsaz F, Geer LY, Geer RC, He J, Gwadz M, Hurwitz DI, Lanczycki CJ, Lu F, Marchler GH, Song JS, Thanki N, Wang Z, Yamashita RA, Zhang D, Zheng C, Bryant SH (2014) CDD: NCBI’s conserved domain database. Nucleic Acids Res 43:D222–D226PubMedPubMedCentralCrossRefGoogle Scholar
- Shimizu M, Fujimoto R, Ying H, Pu ZJ, Ebe Y, Kawanabe T, Saeki N, Taylor JM, Kaji M, Dennis ES, Okazaki K (2014) Identification of candidate genes for Fusarium yellows resistance in Chinese cabbage by differential expression analysis. Plant Mol Biol 85:247–257PubMedCrossRefPubMedCentralGoogle Scholar
- Shirasawa K, Oyama M, Hirakawa H, Sato S, Tabata S, Fujioka T, Kimizuka-Takagi C, Sasamoto S, Watanabe A, Kato M, Kishida Y, Kohara M, Takahashi C, Tsuruoka H, Wada T, Sakai T, Isobe S (2011) An EST-SSR linkage map of Raphanus sativus and comparative genomics of the Brassicaceae. DNA Res 18:221–232PubMedPubMedCentralCrossRefGoogle Scholar
- Simons G, Groenendijk J, Wijbrandi J, Reijans M, Groenen J, Diergaarde P, Van der Lee T, Bleeker M, Onstenk J, de Both M, Haring M, Mes J, Cornelissen B, Zabeau M, Vos P (1998) Dissection of the fusarium I2 gene cluster in tomato reveals six homologs and one active gene copy. Plant Cell 10:1055–1068PubMedPubMedCentralCrossRefGoogle Scholar
- Van Ooijen JW (2006) JoinMap®4, software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, WageningenGoogle Scholar
- Wang S, Basten CJ, Zeng ZB (2006) Windows QTL Cartographer V2.5. User manual. Bioinformatics Research Centre; North Carolina State University, RaleighGoogle Scholar
- Zou Z, Ishida M, Li F, Kakizaki T, Suzuki S, Kitashiba H, Nishio T (2013) QTL analysis using SNP markers developed by next-generation sequencing for identification of candidate genes controlling 4-methylthio-3-butenyl glucosinolate contents in roots of radish, Raphanus sativus L. PloS One 8:e53541PubMedPubMedCentralCrossRefGoogle Scholar