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Theoretical and Applied Genetics

, Volume 132, Issue 8, pp 2439–2460 | Cite as

The genetics of resistance to lettuce drop (Sclerotinia spp.) in lettuce in a recombinant inbred line population from Reine des Glaces × Eruption

  • Bullo Erena Mamo
  • Ryan J. Hayes
  • Maria José Truco
  • Krishna D. Puri
  • Richard W. Michelmore
  • Krishna V. Subbarao
  • Ivan SimkoEmail author
Original Article

Abstract

Key message

Two QTLs for resistance to lettuce drop, qLDR1.1 and qLDR5.1, were identified. Associated SNPs will be useful in breeding for lettuce drop and provide the foundation for future molecular analysis.

Abstract

Lettuce drop, caused by Sclerotinia minor and S. sclerotiorum, is an economically important disease of lettuce. The association of resistance to lettuce drop with the commercially undesirable trait of fast bolting has hindered the integration of host resistance in control of this disease. Eruption is a slow-bolting cultivar that exhibits a high level of resistance to lettuce drop. Eruption also is completely resistant to Verticillium wilt caused by race 1 of Verticillium dahliae. A recombinant inbred line population from the cross Reine des Glaces × Eruption was genotyped by sequencing and evaluated for lettuce drop and bolting in separate fields infested with either S. minor or V. dahliae. Two quantitative trait loci (QTLs) for lettuce drop resistance were consistently detected in at least two experiments, and two other QTLs were identified in another experiment; the alleles for resistance at all four QTLs originated from Eruption. A QTL for lettuce drop resistance on linkage group (LG) 5, qLDR5.1, was consistently detected in all experiments and explained 11 to 25% of phenotypic variation. On LG1, qLDR1.1 was detected in two experiments explaining 9 to 12% of the phenotypic variation. Three out of four resistance QTLs are distinct from QTLs for bolting; qLDR5.1 is pleiotropic or closely linked with a QTL for early bolting; however, the rate of bolting shows only a small effect on the variance in resistance observed at this locus. The SNP markers linked with these QTLs will be useful in breeding for resistance through marker-assisted selection.

Notes

Acknowledgements

We thank Rosa Marchebout, Lorraine Landeros, Jose Orozco, and Rebecca Zhao for assistance in various phases of this research. This research was supported by the US Department of Agriculture’s (USDA) Agricultural Marketing Service through Grant 15-SCBGP-CA-0046. Funding by the California Leafy Greens Research Board also partly supported this work. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the USDA. The mentioning of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA.

Author contribution statement

RJH and KVS conceived the lettuce drop study and obtained funding; RJH generated the population; BEM carried out experiments (phenotyping, mapping, data analyses) and drafted the paper; MJT carried out genotyping and marker identification; KDP conducted genotyping of the Vr1 locus; RWM contributed to data interpretation; RJH, KVS, and IS contributed to phenotyping and data analyses. All authors contributed to writing the paper and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors state that there is no conflict of interest.

Ethical standards

The experiments comply with the laws of the USA, the country in which the study was performed, and the ethical standards of the respective university and employers of the authors.

Supplementary material

122_2019_3365_MOESM1_ESM.xlsx (52 kb)
Supplementary material 1 (XLSX 52 kb)
122_2019_3365_MOESM2_ESM.docx (141 kb)
Supplementary materials 2 to 4 (DOCX 141 kb)

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Authors and Affiliations

  1. 1.Department of Plant PathologyUniversity of California, DavisSalinasUSA
  2. 2.United States Department of Agriculture, Agricultural Research ServiceCrop Improvement and Protection Research UnitSalinasUSA
  3. 3.UC Davis Genome CenterDavisUSA
  4. 4.Departments of Plant Sciences, Molecular and Cellular Biology, Medical Microbiology and ImmunologyUniversity of California, DavisDavisUSA
  5. 5.United States Department of Agriculture, Agricultural Research ServiceForage Seed and Cereal Research UnitCorvallisUSA

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