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Theoretical and Applied Genetics

, Volume 132, Issue 8, pp 2353–2365 | Cite as

GNI-A1 mediates trade-off between grain number and grain weight in tetraploid wheat

  • Guy Golan
  • Idan Ayalon
  • Aviad Perry
  • Gil Zimran
  • Toluwanimi Ade-Ajayi
  • Assaf Mosquna
  • Assaf Distelfeld
  • Zvi PelegEmail author
Original Article

Abstract

Key message

Wild emmer allele of GNI-A1 ease competition among developing grains through the suppression of floret fertility and increase grain weight in tetraploid wheat.

Abstract

Grain yield is a highly polygenic trait determined by the number of grains per unit area, as well as by grain weight. In wheat, grain number and grain weight are usually negatively correlated. Yet, the genetic basis underlying trade-off between the two is mostly unknown. Here, we fine-mapped a grain weight QTL using wild emmer introgressions in a durum wheat background and showed that grain weight is associated with the GNI-A1 gene, a regulator of floret fertility. In-depth characterization of grain number and grain weight indicated that suppression of distal florets by the wild emmer GNI-A1 allele increases weight of proximal grains in basal and central spikelets due to alteration in assimilate distribution. Re-sequencing of GNI-A1 in tetraploid wheat demonstrated the rich allelic repertoire of the wild emmer gene pool, including a rare allele which was present in two gene copies and contained a nonsynonymous mutation in the C-terminus of the protein. Using an F2 population generated from a cross between wild emmer accessions Zavitan, which carries the rare allele, and TTD140, we demonstrated that this unique polymorphism is associated with grain weight, independent of grain number. Moreover, we showed, for the first time, that GNI-A1 proteins are transcriptional activators and that selection targeted compromised activity of the protein. Our findings expand the knowledge of the genetic basis underlying trade-off between key yield components and may contribute to breeding efforts for enhanced grain yield.

Notes

Acknowledgements

We highly appreciate the excellent technical support of the Peleg laboratory members. Specially, we thank N. Teboul for drawing in Fig. 4. This research was supported by the Chief Scientist of the Israel Ministry of Agriculture and Rural Development (Grant #12-01-0005) and the US Agency for International Development Middle East Research and Cooperation (Grant # M34-037).

Author contribution statement

G.G., A.M., A.D. and Z.P. designed the research. G.G., I.A., A.P., G.Z. and T.A-A. performed experiments. G.G., G.Z. and Z.P. analyzed the data. G.G. and Z.P. wrote the manuscript with contributions from all coauthors.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2019_3358_MOESM1_ESM.pdf (2.1 mb)
Supplementary material 1 (PDF 2192 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.The Robert H. Smith Institute of Plant Sciences and Genetics in AgricultureThe Hebrew University of JerusalemRehovotIsrael
  2. 2.School of Plant Sciences and Food SecurityTel Aviv UniversityTel AvivIsrael

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