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Theoretical and Applied Genetics

, Volume 132, Issue 8, pp 2223–2236 | Cite as

BjuWRR1, a CC-NB-LRR gene identified in Brassica juncea, confers resistance to white rust caused by Albugo candida

  • Heena Arora
  • K. Lakshmi Padmaja
  • Kumar Paritosh
  • Nitika Mukhi
  • A. K. Tewari
  • Arundhati Mukhopadhyay
  • Vibha Gupta
  • Akshay K. Pradhan
  • Deepak PentalEmail author
Original Article
  • 225 Downloads

Abstract

Key message

BjuWRR1, a CNL-type R gene, was identified from an east European gene pool line of Brassica juncea and validated for conferring resistance to white rust by genetic transformation.

Abstract

White rust caused by the oomycete pathogen Albugo candida is a significant disease of crucifer crops including Brassica juncea (mustard), a major oilseed crop of the Indian subcontinent. Earlier, a resistance-conferring locus named AcB1-A5.1 was mapped in an east European gene pool line of B. juncea—Donskaja-IV. This line was tested along with some other lines of B. juncea (AABB), B. rapa (AA) and B. nigra (BB) for resistance to six isolates of A. candida collected from different mustard growing regions of India. Donskaja-IV was found to be completely resistant to all the tested isolates. Sequencing of a BAC spanning the locus AcB1-A5.1 showed the presence of a single CC-NB-LRR protein encoding R gene. The genomic sequence of the putative R gene with its native promoter and terminator was used for the genetic transformation of a susceptible Indian gene pool line Varuna and was found to confer complete resistance to all the isolates. This is the first white rust resistance-conferring gene described from Brassica species and has been named BjuWRR1. Allelic variants of the gene in B. juncea germplasm and orthologues in the Brassicaceae genomes were studied to understand the evolutionary dynamics of the BjuWRR1 gene.

Notes

Acknowledgements

We thank Mariam Ibrahim Taom for the help with cloning. The research was supported by the Department of Biotechnology (DBT), Government of India, with grants—BT/IN/Indo-UK/CGAT/12/DP/2014-15 and BT/01/NDDB/UDSC/2016. HA was supported by a research fellowship from the University Grants Commission (UGC). DP was supported by a J. C. Bose Fellowship from the Department of Science & Technology (DST) and by the Council of Scientific and Industrial Research (CSIR) as a Distinguished Scientist.

Author contribution statement

HA, KLP, KP, NM and AM performed the experiments. AKT collected A. candida isolates. AKP and DP supervised the study. HA, VG, AKP and DP drafted the manuscript. All the authors approved the final draft of manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

The experiments were performed according to the laws of India.

Supplementary material

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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of GeneticsUniversity of Delhi South CampusNew DelhiIndia
  2. 2.Centre for Genetic Manipulation of Crop PlantsUniversity of Delhi South CampusNew DelhiIndia
  3. 3.Department of Plant PathologyGovind Ballabh Pant University of Agriculture and TechnologyPantnagarIndia

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