Analysis of recombinant inbred line populations derived from wheat landraces to identify new genes for wheat stem sawfly resistance
Wheat landrace accessions were chosen from areas of the world with historical European wheat stem sawfly (Cephus pygmaeus L.) selection pressure to develop six recombinant inbred line (RIL) populations. Molecular maps were constructed, and resistance due to antibiosis and antixenosis was assessed at sites in Montana naturally infested by Cephus cinctus Norton, the wheat stem sawfly (WSS). Novel QTLs were identified along with QTL previously identified in elite germplasm. A newly identified QTL on chromosome 1B provided a new source for pith-filled solid stems. An allele for resistance on chromosome 4A unrelated to solid stems was identified in four of the six RIL populations. A landrace from Turkey, PI 166471, contained alleles at three QTLs causing high levels of larval mortality. None of the QTLs were related to stem solidness, but their combined effect provided resistance similar to that observed in a solid-stemmed check cultivar. These results show the utility of genetic populations derived from geographically targeted landrace accessions to identify new alleles for insect resistance. New PCR-based molecular markers were developed for introgression of novel alleles for WSS resistance into elite lines. Comparison of results with previous analysis of elite cultivars addresses changes in allele frequencies during the wheat breeding process.
This work was supported by the Agriculture and Food Research Initiative Competitive Grants 2011-68002-30029 (Triticeae-CAP), 2017-67007-25939 (Wheat-CAP) from the USDA National Institute of Food and Agriculture, 2013-67013-21106 from the USDA National Institute of Food and Agriculture, and the Montana Wheat and Barley Committee.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Barrero JM, Cavanagh C, Verbyla KL, Tibbits JFG, Verbyla AP, Huang BE, Rosewarne GM, Stephen S, Wang PH, Whan A, Rigault P, Hayden MJ, Gubler F (2015) Transcriptomic analysis of wheat near-isogenic lines identifies PM19-A1 and A2 as candidates for a major dormancy QTL. Genome Biol 16:93CrossRefGoogle Scholar
- Beres BL, Hill BD, Cárcamo CA, Knodel JJ, Weaver DK, Cuthbert RD (2017) An artificial neural network model to predict wheat stem sawfly cutting in solid-stemmed wheat cultivars. Can J Plant Sci 97:329–336Google Scholar
- Cavanagh C, Chao S, Wang S, Huang BE, Kiani S, Forrest K, Saintenac C, Brown-Guedira G, Akhunova A, See D, Bai G, Pumphrey M, Tomar L, Wong D, Kong S, Reynolds M, Lopez da Silva M, Bockelman H, Talbert LE, Anderson J, Dreisigacker S, Baenziger S, Carter A, Korzun V, Morrell P, Dubcovsky J, Sorrells M, Hayden M, Akhunov EA (2013) Genome-wide comparative diversity uncovers multiple targets of selection for improvement in a worldwide sample of hexaploid wheat landrace and cultivars. Proc Natl Acad Sci 110:8057–8062CrossRefGoogle Scholar
- Chen S, Hoelmer KA, Chen H, Liu A, Shanower TG (2004) A review of wheat stem sawfly (Hymenoptera: Cephidae) in China. J Agric Urban Entomol 21:249–256Google Scholar
- Damania AB (1988) Sampling cereal diversity in Morocco. FAO/IBPGR Plant Genet Res Newsl 72:29–30Google Scholar
- El Bouhssini M, Street K, Amri A, Mackay M, Ogbonnaya FC, Omran A, Abdalla O, Baum M, Dabbous A, Rihawi R (2011) Sources of resistance in bread wheat to Russian wheat aphid (Diuraphis noxia) in Syria using the Focused Identification of Germplasm Strategy (FIGS). Plant Breed 130:96–97CrossRefGoogle Scholar
- Jordan KW, Wang S, He F, Chao S, Lun Y, Paux E, Sourdille P, Sherman J, Akhunova A, Blake NK, Pumphrey MO, Glover K, Dubcovsky J, Talbert L, Akhunov EA (2018) The genetic architecture of genome-wide recombination rate variation in allopolyploid wheat revealed by nested association mapping. Plant J 95:1039–1054CrossRefGoogle Scholar
- Korkmaz EM, Budak M, Hastaoglu Orgen S, Bagda E, Gencer L, Ulgenturk S, Basibuyuk HH (2010) New records and a checklist of Cephidae (Hymenoptera: Insecta) of Turkey with a short biogeographical description. Turk J Zool 34:203–211Google Scholar
- Laamari M, Khelfa L, Coeur d’Acier A (2008) Resistance source to cowpea aphid (Aphis craccivora Koch) in broad bean (Vicia faba L.) Algerian landrace collection. Afr J Biotechnol 7:2486–2490Google Scholar
- Morrill WL (1997) The wheat stem sawfly, Cephus cinctus (Hymenoptera: Cephidae), and associated parasitoids in the northern Great Plains of North America. Trends Entomol 1:171–174Google Scholar
- Platt AW, Farstad CW, Callenbach JA (1948) The reaction of Rescue wheat to sawfly damage. Sci Agric 28:154–161Google Scholar
- SAS Institute, Inc (2010) SAS/STAT 92 user’s guide. SAS Institute, Inc., CaryGoogle Scholar
- Shanower TG, Hoelmer KA (2004) Biological control of wheat stem sawflies: past and future. J Agric Entomol 21:197–221Google Scholar
- Taylor JD, Butler D (2014) ASMap: linkage map construction using the MSTmap algorithm, R package version 0.4-7. https://cran.r-project.org/web/packages/ASMap/
- Varella AC, Talbert LE, Hofland ML, Buteler M, Sherman JD, Blake NK, Heo H-Y, Martin JM, Weaver DK (2016) Alleles at a quantitative trait locus for stem solidness in wheat affect temporal patterns of pith expression and level of resistance to the wheat stem sawfly. Plant Breed 135:546–551CrossRefGoogle Scholar
- Wang S, Wong D, Forrest K, Allen A, Chao S, Huang BE, Maccaferri M, Salvi S, Milner SG, Cattivelli L, Mastrangelo AM, Whan A, Stephen S, Barker G, Wieseke R, Plieske J, International Wheat Genome Sequencing Consortium, Lillemo M, Mather D, Appels R, Dolferus R, Brown-Guedira G, Korol A, Akhunova AR, Feuillet C, Salse J, Morgante M, Pozniak C, Luo MC, Dvorak J, Morell M, Dubcovsky J, Ganal M, Tuberosa R, Lawley C, Mikoulitch I, Cavanagh C, Edwards KJ, Hayden M, Akhunov E (2014) Characterization of polyploid wheat genomic diversity using a high-density 90,000 single nucleotide polymorphism array. Plant Biotechnol J 12:787–796CrossRefGoogle Scholar
- Weaver DK, Sing SE, Runyon JB, Morrill WL (2004) Potential impact of cultural practices on wheat stem sawfly (Hymenoptera: Cephidae) and associated parasitoids. J Agric Urban Entomol 21:271–287Google Scholar