Comprehensive transcriptomics, proteomics, and metabolomics analyses of the mechanisms regulating tiller production in low-tillering wheat
Tiller development in low-tillering wheat is related to several differentially expressed genes, proteins, and metabolites, as determined by an integrated omics approach combining transcriptome analysis, iTRAQ, and HPLC-MS on multiple NILs.
Tillering is an important aspect of plant morphology that affects spike number, thereby contributing to the final crop yield. However, the mechanisms inhibiting tiller production in low-tillering wheat are poorly characterized. To investigate this aspect of wheat biology, two pairs of near-isogenic lines were developed, and an integrated omics approach combining transcriptome analysis, isobaric tags for relative and absolute quantification, and high-performance liquid chromatography-mass spectrometry were used to compare the free-tillering and low-tillering caused by an allele at Qltn.sicau-2D in wheat samples. Overall, 474 genes, 166 proteins, and 28 metabolites were identified as tillering-associated differentially expressed genes, proteins, and metabolites (DEGs, DEPs, and DEMs, respectively). Functional analysis indicated that the abundance of DEGs/DEPs/DEMs was related to lignin and cellulose metabolism, cell division, cell cycle processes, and glycerophospholipid metabolism; three transcription factor families, GRAS, GRF, and REV, might be related to the decrease in tillering in low-tillering wheat. These findings contribute to improve our understanding of the mechanisms responsible for the inhibition of tiller development in low-tillering wheat cultivars.
This study was supported by the National Key Research and Development Program of China (2016YFD0101004), the National Natural Science Foundation of China (31771794), the outstanding Youth Foundation of the Department of Science and Technology of Sichuan Province (2016JQ0040), the Key Technology Research and Development Program of the Department of Science and Technology of Sichuan Province (2016NZ0057), and the International Science & Technology Cooperation Program of the Bureau of Science and Technology of Chengdu China (No. 2015DFA306002015-GH0300008-HZ).
Author Contribution Statement
ZQW and HRS conducted data analysis and drafted the manuscript. SFY, WLZ, JL, and MD performed the phenotypic evaluation and field sampling and helped with data analysis. JM helped to draft the manuscript. YMW participated in the design of the study. YLZ helped to coordinate the study. YXL designed and coordinated this study and revised the manuscript. All authors have read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The experiments comply with the ethical standards in the country in which they were performed.
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