Fine mapping of Aegilops peregrina co-segregating leaf and stripe rust resistance genes to distal-most end of 5DS
Novel rust resistance genes LrP and YrP from Ae. peregrina identified on chromosome 5D and the linked markers will aid deployment of these genes in combination with other major/minor genes.
Aegilops peregrina, a wild tetraploid relative of wheat with genome constitution UUSS, displays genetic variation for resistance to leaf and stripe (yellow) rust. The wheat Ae. peregrina introgression line, IL pau16058, harbouring leaf and stripe rust resistance, was crossed with wheat cv. WL711 to generate an F2:3 mapping population. Inheritance studies on this population indicated the transfer of dominant co-segregating resistance to leaf and stripe rust. Ethyl methane sulphonate mutagenesis of IL pau16058 identified independent loss-of-function mutants for leaf and stripe rust resistance, indicating that the leaf and stripe rust resistance is controlled by independent genes, herein designated LrP and YrP, respectively. A high-resolution genetic map of LrP and YrP was constructed using the Illumina Infinium iSelect 90K wheat array and resistance gene enrichment sequencing (RenSeq) markers. The map spans 4.19 cM on the distal-most region of the short arm of chromosome 5D, consisting of eight SNP markers and one microsatellite marker. LrP and YrP co-segregated with markers BS00163889 and 5DS44573_snp and was flanked distally by the SNP marker BS00129707 and proximally by 5DS149010, defining a 15.71 Mb region in the RefSeq v1.0 genome assembly.
This project was funded by the Sustainable Crop Production Research for International Development (SCPRID) and the Crop Genomics and Technologies (CGAT) programmes from the Department of Biotechnology, Ministry of Science and Technology, Government of India, and the Biotechnology and Biological Sciences Research Council, UK, Grant BT/IN/UK/08/PC/2012 (BB/J012017/1) to PC and CU, Grant BT/IN/Indo-UK/CGAT/14/PC/2014-15 (BBS/E/J/000CA572) to PC and BBHW and the BBSRC Designing Future Wheat Programme (BB/P016855/1). The provision of rust cultures by the Directorate of Wheat Research Regional Research Station, Shimla, is thankfully acknowledged.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Aggarwal R, Kulshreshtha D, Sharma S, Singh VK, Manjunatha C, Bhardwaj SC, Saharan MS (2018) Molecular characterization of Indian pathotypes of Puccinia striiformis f. sp. tritici and multigene phylogenetic analysis to establish inter- and intraspecific relationships. Genet Mol Biol. https://doi.org/10.1590/1678-4685-gmb-2017-0171 Google Scholar
- Bansal M (2017) Fine Mapping and identification of candidate genes for a stripe rust and a leaf rust resistance transferred from Aegilops umbellulata to bread wheat (Triticum aestivum). Dissertation, Punjab Agricultural University, Ludhiana, Punjab, IndiaGoogle Scholar
- Brabham HJ, Hernández-Pinzón I, Holden S, Lorang J, Moscou MJ (2017) An ancient integration in a plant NLR is maintained as a trans-species polymorphism. BiorRxiv: https://doi.org/10.1101/239541
- Cavanagh CR, Chao S, Wang S, Huang BE, Stephen S, Kiani S, Forrest K, Saintenac C, Brown-Guedira GL, Akhunova A, See D, Bai G, Pumphrey M, Tomar L, Akhunov E (2013) Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars. Proc Natl Acad Sci USA 110:8057–8062CrossRefGoogle Scholar
- Chhuneja P, Yadav B, Stirnweis D, Hurni S, Kaur S, Elkot AF, Keller B, Wicker T, Sehgal S, Gill BS, Singh K (2015) Fine mapping of powdery mildew resistance genes PmTb7A.1 and PmTb7A.2. In Triticum boeoticum (Boiss.) using the shotgun sequence assembly of chromosome 7AL. Theor Appl Genet 128:2099–2111CrossRefGoogle Scholar
- Chhuneja P, Kaur S, Dhaliwal HS (2016) Introgression and exploitation of biotic stress tolerance from related wild species in wheat cultivars. In: Rajpal VR et al (eds) Molecular breeding for sustainable crop improvement, sustainable development and biodiversity, vol 11. Springer, Basel, pp 269–324CrossRefGoogle Scholar
- Flor HH (1971) Current status of the gene-for-gene concept. Annu Rev Phytopathol 9:275–296. https://doi.org/10.1146/annurev.py.09.090171.001423 CrossRefGoogle Scholar
- Jupe F, Witek K, Verweij W, Sliwka J, Pritchard L, Etherington GJ (2013) Resistance gene enrichment sequencing (RenSeq) enables reannotation of the NB-LRR gene family from sequenced plant genomes and rapid mapping of resistance loci in segregating populations. Plant J 76:530–544CrossRefGoogle Scholar
- Kiran K, Rawal HC, Dubey H, Jaswal R, Devanna BN, Gupta DK, Bhardwaj SC, Prasad P, Pal D, Chhuneja P, Balasubramanian P, Kumar J, Swami M, Solanke AU, Gaikwad K, Singh NK, Sharma TR (2016) Draft genome of the wheat rust pathogen (Puccinia triticina) unravels genome-wide structural variations during evolution. Genome Biol Evol 8(9):2702–2721CrossRefGoogle Scholar
- Klymiuk V, Yaniv E, Huang L, Raats D, Fatiukha A, Chen S, Feng L, Frenkel Z, Krugman T, Lidzbarsky G, Chang W, Jääskeläinen MJ, Schudoma C, Paulin L, Laine P, Bariana H, Sela H, Saleem K, Sørensen CK, Hovmøller MS, Distelfeld A, Chalhoub B, Dubcovsky J, Korol AB, Schulman AH, Fahima T (2018) Cloning of the wheat Yr15 resistance gene sheds light on the plant tandem kinase-pseudokinase family. Nat Commun 9:3735CrossRefGoogle Scholar
- McIntosh RA (1977) Induced mutations against plant diseases. In: Proceedings of a symposium on the use of induced mutations for improving disease resistance in crop plants; 31 Jan–4 Feb 1977; Vienna. International Atomic Energy Agency, Vienna; 1977. Nature of induced mutations affecting disease reaction in wheat; pp 551–564Google Scholar
- McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Xia XC (2017) Catalogue of gene symbols for wheat: 2017 Supplement. http://www.shigen.nig.ac.jp/wheat/komugi/genes/symbolClassList.jsp
- Molnár I, Vrána J, Burešová V, Cápal P, Farkas A, Darkó E, Cseh A, Kubaláková M, Molnár-Láng M, Doležel J (2016) Dissecting the U, M, S and C genomes of wild relatives of bread wheat (Aegilops spp.) into chromosomes and exploring their synteny with wheat. Plant J 88(3):452–467. https://doi.org/10.1111/tpj.13266 CrossRefGoogle Scholar
- Sears ER (1954) The aneuploids of common wheat. Research Bulletin 572, Missouri Agricultural Experiment Station, University of Missouri, Columbia, p 58Google Scholar
- Sears ER (1972) Chromosome engineering in wheat. In: Stadler genetics symposium, vol 4. University of Missouri, Columbia, pp 23–38Google Scholar
- Singh K, Chhuneja P, Ghai M, Kaur S, Goel RK, Bains NS, Keller B, Dhaliwal HS (2007) Molecular mapping of leaf and stripe rust resistance genes in Triticum monococcum and their transfer to hexaploid wheat. In: Buck H, Nisi JE, Solomon N (eds) Wheat production in stressed environments, 12th edn. Springer, dordrecht, pp 779–786CrossRefGoogle Scholar
- Tiwari VK, Wang S, Danilova T, Koo DH, Vrána J, Kubaláková M, Hribova E, Rawat N, Kalia B, Singh N, Friebe B, Doležel J, Akhunov E, Poland J, Sabir JSM, Gill BS (2015) Exploring the tertiary gene pool of bread wheat: sequence assembly and analysis of chromosome 5Mg of Aegilops geniculata. Plant J 84:733–746CrossRefGoogle Scholar
- Uauy C, Wulff BBH, Dubcovsky J (2017) Combining traditional mutagenesis with new high-throughput sequencing and genome editing to reveal hidden variation in polyploid wheat. Annu Rev Genet 51:435–454. https://doi.org/10.1146/annurev-genet-120116-024533 CrossRefGoogle Scholar
- Wang S, Wong D, Forrest K, Allen A, Chao S, Huang BE, Maccaferri M, Salvi S, Milner SG, Cattivelli L, Mastrangelo AM, Whan A, Stephen S, Barker G, Wieseke R, Plieske J, Lillemo Hayden M, Akhunov E, International Wheat Genome Sequencing Consortium (2014) Characterization of polyploid wheat genomic diversity using a high-density 90000 single nucleotide polymorphism array. Plant Biotechnol J 12:787–796CrossRefGoogle Scholar
- Yu G, Champouret N, Steuernagel B, Olivera PD, Simmons J, Williams C, Johnson R, Moscou MJ, Hernández-Pinzón I, Green P, Sela H, Millet E, Jones JDG, Ward ER, Steffenson BJ, Wulff BBH (2017) Discovery and characterization of two new stem rust resistance genes in Aegilops sharonensis. Theor Appl Genet 130(6):1207–1222. https://doi.org/10.1007/s00122-017-2882-8 CrossRefGoogle Scholar