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Identification and validation of a novel major QTL for all-stage stripe rust resistance on 1BL in the winter wheat line 20828

  • Jian MaEmail author
  • Nana Qin
  • Ben Cai
  • Guoyue Chen
  • Puyang Ding
  • Han Zhang
  • Congcong Yang
  • Lin Huang
  • Yang Mu
  • Huaping Tang
  • Yaxi Liu
  • Jirui Wang
  • Pengfei Qi
  • Qiantao Jiang
  • Youliang Zheng
  • Chunji Liu
  • Xiujin LanEmail author
  • Yuming WeiEmail author
Original Article
  • 108 Downloads

Abstract

Key message

A major, likely novel stripe rust resistance QTL for all-stage resistance on chromosome arm 1BL identified in a 1.76-cM interval using a saturated linkage map was validated in four populations with different genetic backgrounds.

Abstract

Stripe rust is a globally important disease of wheat. Identification and utilization of new resistance genes are essential for breeding resistant cultivars. Wheat line 20828 has exhibited high levels of stripe rust resistance for over a decade. However, the genetics of stripe rust resistance in this line has not been studied. A set of 199 recombinant inbred lines (RILs) were developed from a cross between 20828 and a susceptible cultivar Chuannong 16. The RIL population was genotyped with the Wheat55K SNP (single nucleotide polymorphism) array and SSR (simple sequence repeat) markers and evaluated in four environments with current predominant Puccinia striiformis f. sp. tritici t races including CYR32, CYR33 and CYR34. Four stable QTL were located on chromosomes 1B (2 QTL), 4A and 6A. Among them, the major QTL, QYr.sicau-1B.1 (LOD = 23–28, PVE = 16–39%), was localized to a 1.76-cM interval flanked by SSR markers Xwmc216 and Xwmc156 on chromosome 1BL. Eight resistance genes were previously identified in the physical interval of QYr.sicau-1B.1. Compared with previous studies, QYr.sicau-1B.1 is a new gene for resistant to stripe rust. It was further verified by analysis of the closely linked SSR markers Xwmc216 and Xwmc156 in four other populations with different genetic backgrounds. QYr.sicau-1B.1 reduced the stripe rust disease index by up to 82.8%. Three minor stable QTL (located on chromosomes 1B, 4A and 6A, respectively) also added to the resistance level of QYr.sicau-1B.1. Our results provide valuable information for further fine mapping and cloning as well as molecular-assisted breeding with QYr.sicau-1B.1.

Notes

Acknowledgements

This work is supported by the National Key Research and Development Program of China (2016YFD0102000), the National Natural Science Foundation of China (31601292 and 31570335), the International Science and Technology Cooperation and Exchanges Program of Science and Technology Department of Sichuan Province (2017HH0076) and the Key Projects of Scientific and Technological Activities for Overseas Students of Sichuan Province. We acknowledge the help of Dr. R.A. McIntosh, University of Sydney, with English editing and comments. We thank Dr. Jian Hui Wu, Northwest A&F University, for providing seeds of Express. We thank the anonymous referees for critical reading and revising of this manuscript.

Compliance with ethical standards

Conflict of interest

All authors declare that there is no conflict of interest.

Ethical standards

All experiments and data analyses were conducted in Sichuan, and the 55K SNP genotyping was done in Beijing. All authors contributed to the study and approved the final version for submission. The manuscript has not been submitted to any other journal.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Jian Ma
    • 1
    • 2
    Email author
  • Nana Qin
    • 1
    • 2
  • Ben Cai
    • 3
  • Guoyue Chen
    • 1
    • 2
  • Puyang Ding
    • 1
    • 2
  • Han Zhang
    • 1
    • 2
  • Congcong Yang
    • 1
    • 2
  • Lin Huang
    • 1
    • 2
  • Yang Mu
    • 1
    • 2
  • Huaping Tang
    • 1
    • 2
  • Yaxi Liu
    • 1
    • 2
  • Jirui Wang
    • 1
    • 2
  • Pengfei Qi
    • 1
    • 2
  • Qiantao Jiang
    • 1
    • 2
  • Youliang Zheng
    • 1
    • 2
  • Chunji Liu
    • 4
  • Xiujin Lan
    • 1
    • 2
    Email author
  • Yuming Wei
    • 1
    • 2
    Email author
  1. 1.Triticeae Research InstituteSichuan Agricultural UniversityChengduChina
  2. 2.State Key Laboratory of Crop Genetics of Disease Resistance and Disease ControlSichuan Agricultural UniversityChengduChina
  3. 3.College of AgronomySichuan Agricultural UniversityChengduChina
  4. 4.Commonwealth Scientific and Industrial Research Organization Agriculture and FoodSt LuciaAustralia

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