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Theoretical and Applied Genetics

, Volume 132, Issue 4, pp 1089–1107 | Cite as

Characterisation of barley resistance to rhynchosporium on chromosome 6HS

  • Max Coulter
  • Bianca Büttner
  • Kerstin Hofmann
  • Micha Bayer
  • Luke Ramsay
  • Günther Schweizer
  • Robbie Waugh
  • Mark E. Looseley
  • Anna AvrovaEmail author
Original Article

Abstract

Key message

Major resistance gene to rhynchosporium, Rrs18, maps close to the telomere on the short arm of chromosome 6H in barley.

Abstract

Rhynchosporium or barley scald caused by a fungal pathogen Rhynchosporium commune is one of the most destructive and economically important diseases of barley in the world. Testing of Steptoe × Morex and CIho 3515 × Alexis doubled haploid populations has revealed a large effect QTL for resistance to R. commune close to the telomere on the short arm of chromosome 6H, present in both populations. Mapping markers flanking the QTL from both populations onto the 2017 Morex genome assembly revealed a rhynchosporium resistance locus independent of Rrs13 that we named Rrs18. The causal gene was fine mapped to an interval of 660 Kb using Steptoe × Morex backcross 1 S2 and S3 lines with molecular markers developed from Steptoe exome capture variant calling. Sequencing RNA from CIho 3515 and Alexis revealed that only 4 genes within the Rrs18 interval were transcribed in leaf tissue with a serine/threonine protein kinase being the most likely candidate for Rrs18.

Notes

Acknowledgements

This work was funded by the Scottish Food Security Alliance (SFSA) and the Bavarian State Ministry of Food, Agriculture and Forestry. MB, LR, RW, MEL and AA were supported by the Scottish Government Rural and Environment Science and Analytical Services (RESAS). AA and MB were also funded by the BBSRC-CIRC Project BB/J019569/1. KH was supported by the Federal Office of Agriculture and Food (BLE) under Grant No 28-1-41.009-06.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2018_3262_MOESM1_ESM.xlsx (583 kb)
Supplementary material 1 (XLSX 583 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.The James Hutton InstituteInvergowrie, DundeeScotland, UK
  2. 2.Institute for Crop Science and Plant BreedingBavarian State Research Center for AgricultureFreisingGermany

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