Different loci associated with root and foliar resistance to sudden death syndrome (Fusarium virguliforme) in soybean
Different loci associated with root resistance to F. virguliforme colonization and foliar resistance to phytotoxin damage in soybean.
Use of resistant cultivars is the most efficacious approach to manage soybean sudden death syndrome (SDS), caused by Fusarium virguliforme. The objectives of this study were to (1) map the loci associated with root and foliar resistance to F. virguliforme infection and (2) decipher the relationships between root infection, foliar damage, and plot yield. A mapping population consisting of 153 F4-derived recombinant inbred lines from the cross U01-390489 × E07080 was genotyped by SoySNP6 K BeadChip assay. Both foliar damage and F. virguliforme colonization in roots were investigated in the field, and a weak positive correlation was identified between them. Foliar damage had a stronger negative correlation with plot yield than F. virguliforme colonization. Twelve loci associated with foliar damage were identified, and four of them were associated with multiple traits across environments. In contrast, only one locus associated with root resistance to F. virguliforme colonization was identified and mapped on Chromosome 18. It colocalized with the locus associated with foliar damage in the same environment. The locus on Chromosome 6, qSDS6-2, and the locus on Chromosome 18, qSDS18-1, were associated with resistance to SDS phytotoxins and resistance to F. virguliforme colonization of roots, respectively. Both loci affected plot yield. Foliar damage-related traits, especially disease index, are valuable indicators for SDS resistance breeding because of consistency of the identified loci and their stronger correlation with plot yield. The information provided by this study will facilitate marker-assisted selection to improve SDS resistance in soybean.
This work was supported by North Central Soybean Research Program, Michigan Soybean Promotion Committee, USDA National Institute of Food and Agriculture, Hatch project 1011788 and MSU AgBioResearch. We thank George L. Graef from the Department of Agronomy and Horticulture at the University of Nebraska–Lincoln for providing one of the parental lines, U01-390489. We thank Adam Byrne, Yingdong Bi, Shichen Zhang, Wenyan Du, Jiazheng Yuan, Lihong Li, Wei Xiao, and Zhimin Dong for assistance during the conduction of this research. This study was reviewed by Dr. James Kelly and Dr. Amy Iezzoni from Michigan State University.
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Conflict of interest
On behalf of all authors, the corresponding author states that there is no conflict of interest.
This work complies with the current law of the USA.
- Abdelmajid KM, Meksem K, Wood AJ, Lightfoot DA (2007) Loci underlying SDS and SCN resistance mapped in the “Essex” by “Forrest” soybean recombinant inbred lines. Rev Biol Biotechnol 6:2–10Google Scholar
- Abdelmajid KM, Ramos L, Leandro L et al (2012) The “PI 438489B” by “Hamilton” SNP-based genetic linkage map of soybean [Glycine max (L.) Merr.] identified quantitative trait loci that underlie seedling SDS resistance. J Plant Genome Sci 1:18–30. https://doi.org/10.5147/jpgs.2012.0053 CrossRefGoogle Scholar
- Aoki T, O’donnell K, Homma Y, Lattanzi AR (2003) Sudden-death syndrome of soybean is caused by two morphologically and phylogenetically distinct species within the Fusarium solani species complex—F.virguliforme in North America and F.tucumaniae in South America. Mycologia 95:660–684. https://doi.org/10.2307/3761942 Google Scholar
- Brar HK, Bhattacharyya MK (2012) Expression of a single-chain variable-fragment antibody against a Fusarium virguliforme toxin peptide enhances tolerance to sudden death syndrome in transgenic soybean plants. Mol Plant Microbe Interact 25:817–824. https://doi.org/10.1094/MPMI-12-11-0317 CrossRefGoogle Scholar
- Chang SJC, Doubler TW, Kilo V et al (1996) Two additional loci underlying durable field resistance to soybean sudden death syndrome (SDS). Crop Sci 36:1684–1688. https://doi.org/10.2135/cropsci1996.0011183X003600060044x CrossRefGoogle Scholar
- Chang H-X, Domier LL, Radwan O et al (2016) Identification of multiple phytotoxins produced by Fusarium virguliforme including a phytotoxic effector (FvNIS1) associated with sudden death syndrome foliar symptoms. Mol Plant Microbe Interact 29:96–108. https://doi.org/10.1094/MPMI-09-15-0219-R CrossRefGoogle Scholar
- Fehr WR, Caviness CE, Burmood DT, Pennington JS (1971) Stage of development descriptions for soybeans, Glycine max (L.) Merrill. Crop Sci 11:929–931. https://doi.org/10.2135/cropsci1971.0011183X001100060051x CrossRefGoogle Scholar
- Hnetkovsky N, Chang SJC, Doubler TW et al (1996) Genetic mapping of loci underlying field resistance to soybean sudden death syndrome (SDS). Crop Sci 36:393–400. https://doi.org/10.2135/cropsci1996.0011183X003600020030x CrossRefGoogle Scholar
- Kisha TJ, Sneller CH, Diers BW (1997) Relationship between genetic distance among parents and genetic variance in populations of soybean. Crop Sci 37:1317–1325. https://doi.org/10.2135/cropsci1997.0011183X003700040048x CrossRefGoogle Scholar
- Van Ooijen J (2006) JoinMap 4. Software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, WageningenGoogle Scholar
- Wang S, Basten CJ, Zeng Z-B (2012) Windows QTL cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC. http://statgen.ncsu.edu/qtlcart/WQTLCart.htm