Development and molecular cytogenetic identification of a new wheat-rye 4R chromosome disomic addition line with resistances to powdery mildew, stripe rust and sharp eyespot
A wheat-rye 4R chromosome disomic addition line with resistances to powdery mildew, stripe rust, sharp eyespot and high kernel number per spike was developed and characterized by molecular cytogenetic method as novel resistant germplasm.
Rye (Secale cereale L.), a close relative of common wheat, is an important and valuable gene donor with multiple disease resistance for wheat improvement. However, resistance genes derived from rye have successively lost resistance to pathogens due to the coevolution of pathogen virulence and host resistance. Development and identification of new effective resistance gene sources from rye therefore are of special importance and urgency. In the present study, a wheat-rye line WR35 was produced through distant hybridization, embryo rescue culture, chromosome doubling and backcrossing. WR35 was then proven to be a new wheat-rye 4R disomic addition line using sequential GISH (genomic in situ hybridization), mc-FISH (multicolor fluorescence in situ hybridization) and ND-FISH (non-denaturing FISH) with multiple probes, mc-GISH (multicolor GISH), rye chromosome arm-specific marker analysis and SLAF-seq (specific-locus amplified fragment sequencing) analysis. At the adult stage, WR35 exhibited high levels of resistance to the powdery mildew (Blumeria graminis f. sp. tritici, Bgt) and stripe rust (Puccinia striiformis f. sp. tritici, Pst) pathogens prevalent in China, and a highly virulent isolate of Rhizoctonia cerealis, the cause of wheat sharp eyespot. At the seedling stage, it was highly resistant to 22 of 23 Bgt isolates and four Pst races. Based on its disease responses to different pathogen isolates, WR35 may possess resistance gene(s) for powdery mildew, stripe rust and sharp eyespot, which differed from the known resistance genes from rye. In addition, WR35 was cytologically stable and produced high kernel number per spike. Therefore, WR35 with multi-disease resistances and desirable agronomic traits should serve as a promising bridging parent for wheat chromosome engineering breeding.
Blumeria graminis f. sp. tritici
Chinese Spring wheat
Expressed sequence tag-simple sequence repeat
Fluorescence in situ hybridization
Genomic in situ hybridization
Specific-locus amplified fragment sequencing
Polymerase chain reaction
Puccinia striiformis f. sp. tritici
The authors thank Dr. Yilin Zhou and Dr. Shichang Xu from the State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China, and Dr. Shibin Cai from Institute of Food Crops, Jiangsu Academy of Agricultural Science, Nanjing, China, for conducting assessment of the reactions to powdery mildew, stripe rust and sharp eyespot. This research was supported by the National Key Research and Development Program of China (Nos. 2016YFD0102002 and 2016YFD0100102) and the National Natural Science Foundation of China (No. 31771793).
Compliance with ethical standards
Conflict of interest
The authors declare that our experiments comply with the current laws of China and we have no conflicts of interest.
This article does not contain any studies that were performed with human participants or animals by any of the authors.
- An DG, Zhong GC, Li JM, Wang ZG, Wang YM, Ji J (2003) Chromosome doubling methods of immature embryo plants of wheat distant hybridization. Acta Agron Sin 29:955–957Google Scholar
- He ZH, Xia XC, Chen XM, Zhang Y, Zhang Y, Yan J, Cao SH, Rasheed A (2015) Application of molecular markers in plant quality and disease resistance breeding. In: The seventh national symposium on wheat genetics and breeding, Zhengzhou, ChinaGoogle Scholar
- Koeszegi B, Farshadfar E, Vagujfalvi A, Sutka J (1996) Drought tolerance studies on wheat/rye disomic chromosome addition lines. Acta Agron Hung 44:121–126Google Scholar
- Lind V (1982) Analysis of the resistance of wheat-rye addition lines to powdery mildew of wheat (Erysiphe graminis f. sp. tritici). Tagungsbericht Akademie Der Landwirtschaftswissenschaften Der DdrGoogle Scholar
- Schlegel RHJ (2016) Current list of wheats with rye and alien introgression. V05–16, pp 1–18. http://www.rye-gene-mapde/rye-introgression
- Schneider A, Rakszegi M, Molnár-Láng M, Szakács É (2016) Production and cytomolecular identification of new wheat-perennial rye (Secale cereanum) disomic addition lines with yellow rust resistance (6R) and increased arabinoxylan and protein content (1R, 4R, 6R). Theor Appl Genet 129:1045–1059CrossRefGoogle Scholar
- Sheng BQ, Duan XY (1991) Improvement of scale 0–9 method for scoring adult plant resistance to powdery mildew of wheat. Beijing Agric Sci 1:38–39Google Scholar
- Shi JR, Wang YZ, Chen HG, Shen YW (2000) Screening techniques and evaluation of wheat resistance to sharp eyespot caused by Rhizoctonia cerealis. Acta Phytophylacica Sin 27(2):107–112Google Scholar
- Si QM, Zhang XX, Duan XY, Sheng BQ, Zhou YL (1992) On gene analysis and classification of powdery mildew (Erysiphe graminis f. sp. tritici) resistant wheat varieties. Acta Phytopathol Sin 22:349–355Google Scholar
- Sidhu MC, Satija CK, Sharma I (2001) Screening of wheat-rye addition lines for Karnal bunt resistance. Crop Improv 28:214–217Google Scholar
- Zhao RH, Wang HY, Xiao J, Bie TD, Cheng SH, Jia Q, Yuan CX, Zhang RQ, Cao AZ, Chen PD, Wang XE (2013) Induction of 4VS chromosome recombinants using the CS ph1b mutant and mapping of the wheat yellow mosaic virus resistance gene from Haynaldia villosa. Theor Appl Genet 126:2921–2930CrossRefGoogle Scholar
- Zhong GC, Mu SM, Zhang ZB (2002) Wheat distant hybridization. Chinese Science Press, BeijingGoogle Scholar
- Zhou YL, Duan XY, Gang C, Sheng BQ, Ying Z (2002) Analyses of resistance genes of 40 wheat cultivars or lines to wheat powdery mildew. Acta Phytopathol Sin 32:301–305Google Scholar
- Zhuang QS (2003) Chinese wheat improvement and pedigree analysis. Chinese Agriculture Press, BeijingGoogle Scholar