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Theoretical and Applied Genetics

, Volume 132, Issue 1, pp 137–147 | Cite as

Construction of a high-density linkage map and QTL detection of downy mildew resistance in Vitis aestivalis-derived ‘Norton’

  • Surya Sapkota
  • Li-Ling Chen
  • Shanshan Yang
  • Katie E. Hyma
  • Lance Cadle-Davidson
  • Chin-Feng HwangEmail author
Original Article
  • 206 Downloads

Abstract

Key message

A major QTL for downy mildew resistance was detected on chromosome 18 (Rpv27) in Vitis aestivalis-derived ‘Norton’ based on a high-resolution linkage map with SNP and SSR markers as well as 2 years of field and laboratory phenotyping data.

Abstract

Grapevine downy mildew caused by the oomycete Plasmopara viticola is one of the most widespread and destructive diseases, particularly in humid viticultural areas where it damages green tissues and defoliates vines. Traditional Vitis vinifera wine grape cultivars are susceptible to downy mildew whereas several North American and a few Asian cultivars possess various levels of resistance to this disease. To identify genetic determinants of downy mildew resistance in V. aestivalis-derived ‘Norton,’ a mapping population with 182 genotypes was developed from a cross between ‘Norton’ and V. vinifera ‘Cabernet Sauvignon’ from which a consensus map was constructed via 411 simple sequence repeat (SSR) markers. Using genotyping-by-sequencing, 3825 single nucleotide polymorphism (SNP) markers were also generated. Of these, 1665 SNP and 407 SSR markers were clustered into 19 linkage groups in 159 genotypes, spanning a genetic distance of 2203.5 cM. Disease progression in response to P. viticola was studied in this population for 2 years under both laboratory and field conditions, and strong correlations were observed among data sets (Spearman correlation coefficient = 0.57–0.79). A quantitative trait loci (QTL) analysis indicated a resistance locus on chromosome 18, here named Rpv27, explaining 33.8% of the total phenotypic variation. Flanking markers closely linked with the trait can be further used for marker-assisted selection in the development of new cultivars with resistance to downy mildew.

Notes

Acknowledgements

The authors thank S. Jacob Schneider, Marilyn Odneal and Kevin Fort for valuable discussions and constructive comments on the manuscript. This project was supported by Agriculture and Food Research Initiative Competitive Grant, Award No. 2013-67014-21360, and Specialty Crop Research Initiative Competitive Grant, Award No. 2011-51181-30635, of the USDA National Institute of Food and Agriculture.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Surya Sapkota
    • 1
    • 2
    • 6
  • Li-Ling Chen
    • 1
  • Shanshan Yang
    • 3
  • Katie E. Hyma
    • 4
  • Lance Cadle-Davidson
    • 5
  • Chin-Feng Hwang
    • 1
    Email author
  1. 1.State Fruit Experiment Station at Mountain Grove Campus, Darr College of AgricultureMissouri State UniversitySpringfieldUSA
  2. 2.Division of Plant SciencesUniversity of MissouriColumbiaUSA
  3. 3.Bioinformatics Core Facility, The Biodesign InstituteArizona State UniversityTempeUSA
  4. 4.Bioinformatics Facility, Institute of BiotechnologyCornell UniversityIthacaUSA
  5. 5.USDA-ARS Grape Genetics Research UnitGenevaUSA
  6. 6.Plant Pathology and Plant Microbe Biology Section, School of Integrative Plant Science, NYS Agricultural Experiment StationCornell UniversityGenevaUSA

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