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Fine mapping and candidate gene identification of the genic male-sterile gene ms3 in cabbage 51S

Abstract

Key message

The ms3 gene responsible for a male-sterile phenotype in cabbage was mapped to a 187.4-kb genomic fragment. The gene BoTPD1, a homolog of Arabidopsis TPD1, was identified as a strong candidate gene.

Abstract

Cabbage 51S is a spontaneous male-sterile mutant. Phenotypic investigation revealed defects in anther cell differentiation, with failure to form the tapetum layer and complete abortion of microsporocytes before the tetrad stage. Genetic analysis indicated that this male sterility was controlled by a single recessive gene, ms3. Using an F2 population, we mapped ms3 to a 187.4-kb interval. BoTPD1 was identified as a candidate from this interval. Sequence analysis revealed an intronic 182-bp insertion in 51S that interrupted the conserved motif at the 5′ splicing site of the third intron, possibly resulting in a truncated transcript. Analyses of BoTPD1 homologous proteins revealed evolutionarily conserved roles in anther cell fate determination during reproductive development. RT-PCR showed that BoTPD1 was expressed in various tissues, excluding the root, and high expression levels were detected in anthers and buds. A BoTPD1-specific marker based on the 182-bp insertion cosegregated with male sterility and can be used for marker-assisted selection.

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Acknowledgements

This work was supported by grants from the National Key Research and Development Program of China (2017YFD0101804), the Central Public-interest Scientific Institution Basal Research Fund (No. 1610102017013), the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS) and the earmarked fund for the Modern Agro-Industry Technology Research System, China (nycytx-35-gw01). The work reported here was performed in the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Beijing 100081, China.

Author information

Correspondence to Zhiyuan Fang or Honghao Lv.

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The authors declare that they have no conflicts of interest to disclose.

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The authors declare that all experiments comply with the current laws of China.

Additional information

Communicated by Carlos F. Quiros.

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Han, F., Yuan, K., Kong, C. et al. Fine mapping and candidate gene identification of the genic male-sterile gene ms3 in cabbage 51S. Theor Appl Genet 131, 2651–2661 (2018). https://doi.org/10.1007/s00122-018-3180-9

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