A new leaf rust resistance gene Lr79 mapped in chromosome 3BL from the durum wheat landrace Aus26582
A new leaf rust resistance gene Lr79 has been mapped in the long arm of chromosome 3B and a linked marker was identified for marker-assisted selection.
Aus26582, a durum wheat landrace from the A. E. Watkins Collection, showed seedling resistance against durum-specific and common wheat-specific Puccinia triticina (Pt) pathotypes. Genetic analysis using a recombinant inbred line (RIL) population developed from a cross between Aus26582 and the susceptible parent Bansi with Australian Pt pathotype showed digenic inheritance and the underlying loci were temporarily named LrAW2 and LrAW3. LrAW2 was located in chromosome 6BS and this study focused on characterisation of LrAW3 using RILs lacking LrAW2. LrAW3 was incorporated into the DArTseq map of Aus26582/Bansi and was located in chromosome 3BL. Markers linked with LrAW3 were developed from the chromosome survey sequence contig 3B_10474240 in which closely-linked DArTseq markers 1128708 and 3948563 were located. Although bulk segregant analysis (BSA) with the 90 K Infinium array identified 51 SNPs associated with LrAW3, only one SNP-derived KASP marker mapped close to the locus. Deletion bin mapping of LrAW3-linked markers located LrAW3 between bins 3BL11-0.85-0.90 and 3BL7-0.63. Since no other all stage leaf rust resistance gene is located in chromosome 3BL, LrAW3 represented a new locus and was designated Lr79. Marker sun786 mapped 1.8 cM distal to Lr79 and Aus26582 was null for this locus. However, the marker can be reliably scored as it also amplifies a monomorphic fragment that serves as an internal control to differentiate the null status of Aus26582 from reaction failure. This marker was validated among a set of durum and common wheat cultivars and was shown to be useful for marker-assisted selection of Lr79 at both ploidy levels.
Naeela Qureshi acknowledges the University of Sydney for the USydIS award to pursue PhD studies. We thank the Australian Centre for International Agricultural Research (ACIAR), Grains Research and Development Corporation (GRDC) Australia, for financial support. The authors thank the International Wheat Genome Sequencing Consortium for pre-publication access to IWGSC RefSeq v1.0.
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Conflict of interest
All authors have read the manuscript and declare that they have no conflicts of interest.
- Bariana HS, Miah H, Brown GN, Willey N, Lehmensiek A (2007) Molecular mapping of durable rust resistance in wheat and its implication in breeding. In: Buck HT, Nisi JE, Salomon N (eds) Wheat production in stressed environments. Developments in plant breeding, vol 12. Springer, Heidelberg, pp 723–728CrossRefGoogle Scholar
- Chhetri M (2015) Molecular mapping and genetic characterization of rust resistance in wheat. PhD Thesis. The University of Sydney, AustraliaGoogle Scholar
- Desiderio F, Guerra D, Mastrangelo AM, Rubiales D, Pasquini M, Simeone R, Blanco A, Cattivelli L, Valè G (2014) Genetic basis of resistance to leaf rust in tetraploid wheats. In: Porceddu E, Damania AB, Qualset CO (eds) Proceedings of the international symposium on genetics and breeding of durum wheat, vol 110. CIHEAM, Bari, pp 447–452Google Scholar
- Herrera-Foessel SA, Singh RP, Huerta-Espino J, Salazar VC, Lagudah ES (2011) First report of slow rusting gene Lr46 in durum wheat. Borlaug Global Rust Initiative, 13–16 June 2011 Technical Workshop, St PaulGoogle Scholar
- Kandiah P (2017) Characterization and molecular mapping of leaf rust and stripe rust resistance in wheat. PhD Thesis. The University of SydneyGoogle Scholar
- Kassa MT, You FM, Hiebert CW, Pozniak CJ, Fobert PR, Sharpe AG, Menzies JG, Humphreys DG, Rezac Harrison N, Fellers JP, McCallum BD, McCartney CA (2017) Highly predictive SNP markers for efficient selection of the wheat leaf rust resistance gene Lr16. BMC Plant Biol 17:45CrossRefPubMedPubMedCentralGoogle Scholar
- McIntosh RA, Dubcovsky J, Rogers JW, Morris C, Appels R, Xia CX (2016) Catalogue of gene symbols for wheat: 2015–2016 supplement. https://shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2015.pdf
- Miller TE, Reader SM, Ambrose MJ (2000) The Watkins wheat collection. Ann Wheat Newslett 46:172Google Scholar
- Singh B, Bansal U, Hare R, Bariana H (2013) Genetic analysis of durable adult plant stripe rust resistance in durum wheat cultivars. Aust J Crop Sci 7:550Google Scholar
- Tekdal S (2014) Importance of durum wheat breeding in terms of Bulghur in Southeastern Anatolian Region of Turkey. In: Porceddu E, Damania AB, Qualset CO (eds) Proceedings of the International Symposium on Genet Breed of Durum Wheat. CIHEAM, Bari, pp 589–594. http://om.ciheam.org/om/pdf/a110/00007121.pdf
- Wang S, Basten CJ, Zeng ZB (2011) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, RaleighGoogle Scholar
- Wang S, Wong D, Forrest K, Allen A, Chao S, Huang BE, Maccaferri M, Salvi S, Milner SG, Cattivelli L, Mastrangelo AM, Whan A, Stephen S, Barker G, Wieseke R, Plieske J, International Wheat Genome Sequencing Consortium, Lillemo M, Mather D, Appels R, Dolferus R, Brown-Guedira G, Korol A, Akhunova AR, Feuillet C, Salse J, Morgante M, Pozniak C, Luo MC, Dvorak J, Morell M, Dubcovsky J, Ganal M, Tuberosa R, Lawley C, Mikoulitch I, Cavanagh C, Edwards KJ, Hayden M, Akhunov E (2014) Characterization of polyploid wheat genomic diversity using a high-density 90,000 single nucleotide polymorphism array. Plant Biotechnol J 12:787–796CrossRefPubMedPubMedCentralGoogle Scholar