Theoretical and Applied Genetics

, Volume 131, Issue 5, pp 1091–1098 | Cite as

A new leaf rust resistance gene Lr79 mapped in chromosome 3BL from the durum wheat landrace Aus26582

  • Naeela Qureshi
  • Harbans Bariana
  • Vikas Venu Kumran
  • Sivasamy Muruga
  • Kerrie L. Forrest
  • Mathew J. Hayden
  • Urmil Bansal
Original Article

Abstract

Key message

A new leaf rust resistance gene Lr79 has been mapped in the long arm of chromosome 3B and a linked marker was identified for marker-assisted selection.

Abstract

Aus26582, a durum wheat landrace from the A. E. Watkins Collection, showed seedling resistance against durum-specific and common wheat-specific Puccinia triticina (Pt) pathotypes. Genetic analysis using a recombinant inbred line (RIL) population developed from a cross between Aus26582 and the susceptible parent Bansi with Australian Pt pathotype showed digenic inheritance and the underlying loci were temporarily named LrAW2 and LrAW3. LrAW2 was located in chromosome 6BS and this study focused on characterisation of LrAW3 using RILs lacking LrAW2. LrAW3 was incorporated into the DArTseq map of Aus26582/Bansi and was located in chromosome 3BL. Markers linked with LrAW3 were developed from the chromosome survey sequence contig 3B_10474240 in which closely-linked DArTseq markers 1128708 and 3948563 were located. Although bulk segregant analysis (BSA) with the 90 K Infinium array identified 51 SNPs associated with LrAW3, only one SNP-derived KASP marker mapped close to the locus. Deletion bin mapping of LrAW3-linked markers located LrAW3 between bins 3BL11-0.85-0.90 and 3BL7-0.63. Since no other all stage leaf rust resistance gene is located in chromosome 3BL, LrAW3 represented a new locus and was designated Lr79. Marker sun786 mapped 1.8 cM distal to Lr79 and Aus26582 was null for this locus. However, the marker can be reliably scored as it also amplifies a monomorphic fragment that serves as an internal control to differentiate the null status of Aus26582 from reaction failure. This marker was validated among a set of durum and common wheat cultivars and was shown to be useful for marker-assisted selection of Lr79 at both ploidy levels.

Notes

Acknowledgements

Naeela Qureshi acknowledges the University of Sydney for the USydIS award to pursue PhD studies. We thank the Australian Centre for International Agricultural Research (ACIAR), Grains Research and Development Corporation (GRDC) Australia, for financial support. The authors thank the International Wheat Genome Sequencing Consortium for pre-publication access to IWGSC RefSeq v1.0.

Compliance with ethical standards

Conflict of interest

All authors have read the manuscript and declare that they have no conflicts of interest.

Supplementary material

122_2018_3060_MOESM1_ESM.pdf (75 kb)
Supplementary material 1 (PDF 74 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.The University of Sydney Plant Breeding InstituteSchool of Life and Environmental SciencesCobbittyAustralia
  2. 2.ICAR-Indian Agricultural Research Institute Regional StationWellingtonIndia
  3. 3.Agriculture, Energy & Resources, Department of Economic Development, Jobs, Transport and ResourcesAgriBioBundooraAustralia

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