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A MYB transcription factor is a candidate to control pungency in Capsicum annuum

  • Koeun Han
  • Siyoung Jang
  • Joung-Ho Lee
  • Do-Gyeong Lee
  • Jin-Kyung Kwon
  • Byoung-Cheorl KangEmail author
Original Article
  • 185 Downloads

Abstract

Key message

Identification of a novel pungency-controlling gene Pun3, which acts as a master regulator of capsaicinoid biosynthetic genes in Capsicum annuum.

Abstract

Capsaicinoid is a unique compound that gives hot peppers (Capsicum spp.) their spicy taste. The Pun1 and Pun2 loci are known to control pungency in Capsicum species. Whereas Pun1 encodes an acyltransferase, the identity of Pun2 is currently unknown. Here, we used recombinant inbred lines and F2 plants derived from a cross between the non-pungent C. annuum accession ‘YCM334’ and the pungent C. annuum cultivar ‘Tean’ to identify a novel non-pungency locus. Inheritance studies showed that non-pungency in C. annuum ‘YCM334’ is controlled by a single recessive gene, which we named Pun3. Using a high-density SNP map derived from genotyping-by-sequencing, Pun3 was mapped to chromosome 7. By comparing physical information about the Pun3 region in the C. annuum ‘Zunla-1’ and C. chinense ‘PI159236’ reference genomes, we identified candidate genes in this target region. One cDNA sequence from ‘PI159236’ was homologous to an unannotated gene in ‘Zunla-1.’ This sequence was also homologous to CaMYB31, which is expressed only in ‘Tean’ and harbors one stop codon in the non-pungent accession ‘YCM334.’ RNA-Seq analysis showed that major structural genes in the capsaicinoid biosynthetic pathway were significantly downregulated in ‘YCM334’ compared to pungent pepper. Therefore, CaMYB31 is a candidate gene for Pun3, which may act as a master regulator of capsaicinoid biosynthetic genes in pepper.

Notes

Acknowledgements

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, and Forestry (IPET) through the Agriculture, Food, and Rural Affairs Research Center Support Program (Vegetable Breeding Research Center, 710011-03), funded by the Ministry of Agriculture, Food, and Rural Affairs (MAFRA). This work was carried out with the support of the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01322901), Rural Development Administration, Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

The experiments were performed according to the laws of Germany.

Supplementary material

122_2018_3275_MOESM1_ESM.docx (4.3 mb)
Supplementary material 1 (DOCX 4403 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Koeun Han
    • 1
  • Siyoung Jang
    • 1
  • Joung-Ho Lee
    • 1
  • Do-Gyeong Lee
    • 1
  • Jin-Kyung Kwon
    • 1
  • Byoung-Cheorl Kang
    • 1
    Email author
  1. 1.Department of Plant Science, Plant Genomics and Breeding Institute, and Vegetable Breeding Research Center, College of Agriculture and Life SciencesSeoul National UniversitySeoulRepublic of Korea

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