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Development of Brassica oleracea-nigra monosomic alien addition lines: genotypic, cytological and morphological analyses

Abstract

Key message

We report the development and characterization of Brassica oleracea - nigra monosomic alien addition lines (MAALs) to dissect the Brassica B genome.

Abstract

Brassica nigra (2n = 16, BB) represents the diploid Brassica B genome which carries many useful genes and traits for breeding but received limited studies. To dissect the B genome from B. nigra, the triploid F1 hybrid (2n = 26, CCB) obtained previously from the cross B. oleracea var. alboglabra (2n = 18, CC) × B. nigra was used as the maternal parent and backcrossed successively to parental B. oleracea. The progenies in BC1 to BC3 generations were analyzed by the methods of FISH and SSR markers to screen the monosomic alien addition lines (MAALs) with each of eight different B-genome chromosomes added to C genome (2n = 19, CC + 1B1−8), and seven different MAALs were established, except for the one with chromosome B2 which existed in one triple addition. Most of these MAALs were distinguishable morphologically from each other, as they expressed the characters from B. nigra differently and at variable extents. The alien chromosome remained unpaired as a univalent in 86.24% pollen mother cells at diakinesis or metaphase I, and formed a trivalent with two C-genome chromosomes in 13.76% cells. Transmission frequency of all the added chromosomes was far higher through the ovules (averagely 14.40%) than the pollen (2.64%). The B1, B4 and B5 chromosomes were transmitted by female at much higher rates (22.38–30.00%) than the other four (B3, B6, B7, B8) (5.04–8.42%). The MAALs should be valuable for exploiting the genome structure and evolution of B. nigra.

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Acknowledgements

We are grateful to Susan J. Armstrong (University of Birmingham, Birmingham, United Kingdom) for providing the clone BoB014O06, and to three anonymous reviewers for constructive comments. The study was supported by NSFC (Grant No. 31371656).

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Correspondence to Cheng Cui or Zaiyun Li.

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The authors declare that they have no conflict of interest.

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Communicated by P. Heslop-Harrison.

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Tan, C., Cui, C., Xiang, Y. et al. Development of Brassica oleracea-nigra monosomic alien addition lines: genotypic, cytological and morphological analyses. Theor Appl Genet 130, 2491–2504 (2017). https://doi.org/10.1007/s00122-017-2971-8

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