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Confirmation of delayed canopy wilting QTLs from multiple soybean mapping populations

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Abstract

Key message

QTLs for delayed canopy wilting from five soybean populations were projected onto the consensus map to identify eight QTL clusters that had QTLs from at least two independent populations.

Abstract

Quantitative trait loci (QTLs) for canopy wilting were identified in five recombinant inbred line (RIL) populations, 93705 KS4895 × Jackson, 08705 KS4895 × Jackson, KS4895 × PI 424140, A5959 × PI 416937, and Benning × PI 416937 in a total of 15 site-years. For most environments, heritability of canopy wilting ranged from 0.65 to 0.85 but was somewhat lower when averaged over environments. Putative QTLs were identified with composite interval mapping and/or multiple interval mapping methods in each population and positioned on the consensus map along with their 95 % confidence intervals (CIs). We initially found nine QTL clusters with overlapping CIs on Gm02, Gm05, Gm11, Gm14, Gm17, and Gm19 identified from at least two different populations, but a simulation study indicated that the QTLs on Gm14 could be false positives. A QTL on Gm08 in the 93705 KS4895 × Jackson population co-segregated with a QTL for wilting published previously in a Kefeng1 × Nannong 1138-2 population, indicating that this may be an additional QTL cluster. Excluding the QTL cluster on Gm14, results of the simulation study indicated that the eight remaining QTL clusters and the QTL on Gm08 appeared to be authentic QTLs. QTL × year interactions indicated that QTLs were stable over years except for major QTLs on Gm11 and Gm19. The stability of QTLs located on seven clusters indicates that they are possible candidates for use in marker-assisted selection.

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Acknowledgments

The authors gratefully acknowledge financial support for this research from the United Soybean Board. This work was also supported in part by the United States Department of Agriculture–Agriculture Research Service (USDA-ARS) project number 6066-21220-012-00D.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Correspondence to Larry C. Purcell.

Additional information

Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply approval or the exclusion of other products that may also be suitable.

Communicated by I. Rajcan.

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Hwang, S., King, C.A., Ray, J.D. et al. Confirmation of delayed canopy wilting QTLs from multiple soybean mapping populations. Theor Appl Genet 128, 2047–2065 (2015). https://doi.org/10.1007/s00122-015-2566-1

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Keywords

  • Recombinant Inbred Line
  • Composite Interval Mapping
  • Plant Introduction
  • Favorable Allele
  • Putative QTLs