Genetic analysis and gene mapping of the 4-methylthio-3-butenyl glucosinolate-less trait of white radish were performed and a white radish cultivar with new glucosinolate composition was developed.
A spontaneous mutant having significantly low 4-methylthio-3-butenyl glucosinolate (4MTB-GSL) content was identified from a landrace of Japanese white radish (Raphanus sativus L.) through intensive evaluation of glucosinolate profiles of 632 lines including genetic resources and commercial cultivars using high-performance liquid chromatography (HPLC) analysis. A line lacking 4MTB-GSL was developed using the selected mutant as a gene source. Genetic analyses of F1, F2, and BC1F1 populations of this line suggested that the 4MTB-GSL-less trait is controlled by a single recessive allele. Using SNP and SCAR markers, 96 F2 plants were genotyped, and a linkage map having nine linkage groups with a total map distance of 808.3 cM was constructed. A gene responsible for the 4MTB-GSL-less trait was mapped between CL1753 and CL5895 at the end of linkage group 1. The genetic distance between these markers was 4.2 cM. By selfing and selection of plants lacking 4MTB-GSL, a new cultivar, ‘Daikon parental line No. 5', was successfully developed. This cultivar was characterized by glucoerucin, which accounted for more than 90 % of the total glucosinolates (GSLs). The total GSL content in roots was ca. 12 μmol/g DW, significantly lower than those in common white radish cultivars. Significance of this line in radish breeding is discussed.
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Banga O (1976) Radish, Raphanus sativus (Cruciferae). In: Simmonds NW (ed) Evolution of Crop Plants. Longman, London, pp 60–62
Bidart-Bouzat M, Kliebenstein DJ (2008) Differential levels of insect herbivory in the field associated with genotypic variation in glucosinolates in Arabidopsis thaliana. J Chem Ecol 34:1026–1037
Bjerg B, Sørensen H (1987) Quantitative analysis of glucosinolates and HPLC of intact glucosinolates. In: Wathelet J-P (ed) Glucosinolates in rapeseeds: Analytical aspects. Martinus Nijhoff Publishers, Dordrecht, pp 125–150
Bones AM, Rossiter JT (1996) The myrosinase-glucosinolate system, its organisation and biochemistry. Physiol Plantarum 97:194–208
Carlson DG, Axenbichler ME, van Etten CH (1985) Glucosinolate in radish cultivars. J Amer Soc Hort Sci 110:634–638
Clarke DB (2010) Glucosinolates, structures and analysis in food. Analytical Methods 2:310–325
Cohen JH, Kristal AR, Stanford JL (2000) Fruit and vegetable intakes and prostate cancer risk. J Natl Cancer Inst 92:61–68
Ediage EN, Di Mavungu JD, Scippo ML, Schneider YJ, Larondelle Y, Callebaut A, Robbens J, Van Peteghem C, De Saeger S (2011) Screening, identification and quantification of glucosinolates in black radish (Raphanus sativus L. niger) based dietary supplements using liquid chromatography coupled with a photodiode array and liquid chromatography-mass spectrometry. J Chromatogr A 1218:4395–4405
Fahey JW, Zalcmann AT, Talalay P (2001) The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56:5–51
Fenwick GR, Heaney RK, Mullin WJ (1983) Glucosinolate and their breakdown products in food and plants. Crit Rev Food Sci Nutr 18:123–201
Friis P, Kjær A (1966) 4-Methylthio-3-butenyl isothiocyanate, the pungent principle of radish root. Acta Chem Scand 20:698–705
Gao M, Li G, McCombie W, Quiros C (2005) Comparative analysis of a transposon-rich Brassica oleracea BAC clone with its corresponding sequence in A. thaliana. Theor Appl Genet 111:949–955
Giamoustaris A, Mithen R (1996) Genetics of aliphatic glucosinolates. IV. Side-chain modification in Brassica oleracea. Theor Appl Genet 93:1006–1010
Halkier BA, Gershenzon J (2006) Biology and biochemistry of glucosinolates. Ann. Rev. Plant Biology 57:303–333
Herr I, Büchler MW (2010) Dietary constituents of broccoli and other cruciferous vegetables: implications for prevention and therapy of cancer. Cancer Treat Rev 36:377–383
Hirani AH, Li G, Zelmer CD, McVetty PBE, Asif M, Goyal A (2012) Molecular genetics of glucosinolate biosynthesis in Brassicas: Genetic manipulation and application aspects. In: Goyal A (ed) Crop Plant. doi: 10.5772/45646. Available from: http://www.intechopen.com/books/crop-plant/molecular-geneticsof-glucosinolate-biosynthesis-in-brassicas
Ishida M, Morimitsu Y (2013) Chemical changes of the breakdown compounds of glucosinolate in processed food of the daikon without containing 4-methylthio-3-butenyl glucosinolate. J Japan Associ Odor Environ 44:307–314 (In Japanese)
Ishida M, Takahata Y, Kaizuma N (2003) Simple and rapid method for the selection of individual rapeseed plants low in glucosinolates. Breed Sci 53:291–296
Ishida M, Kakizaki T, Ohara T, Morimitsu Y (2011) Development of a simple and rapid extraction method of glucosinolates from radish roots. Breed Sci 61:208–211
Ishida M, Nagata M, Ohara T, Kakizaki T, Hatakeyama K, Nishio T (2012) Small variation of glucosinolate composition in Japanese cultivars of radish (Raphanus sativus L.) requires simple quantitative analysis for breeding of glucosinolate component. Breed Sci 62:63–70
Kitamura S (1958) Varieties and transitions of radish. In: Nishiyama I (ed) Japanese radish. Jpn Soc from Sci Tokyo, Japan, pp 1–19 (in Japanese)
Kitashiba H, Li F, Hirakawa H, Kawanabe T, Zou Z, Hasegawa Y, Tonosaki K, Shirasawa S, Fukushima A, Yokoi S, Takahata Y, Kakizaki T, Ishida M, Okamoto S, Sakamoto K, Shirasawa K, Tabata S, Nishio T (2014) Draft Sequences of the radish (Raphanus sativus L.) Genome. DNA Res 21:481–490
Kliebenstein DJ, Lambrix VM, Reichelt M, Gershenzon J, Mitchell-Olds T (2001) Gene duplication in the diversification of secondary metabolism: tandem 2-oxoglutarate-dependent dioxygenases control glucosinolate biosynthesis in Arabidopsis. Plant Cell 13:681–693
Kroymann J, Textor S, Tokuhisa JG, Falk KL, Bartram S, Gershenzon J, Mitchell-Olds T (2001) A gene controlling variation in Arabidopsis glucosinolate composition is part of the methionine chain elongation pathway. Plant Phys 127:1077–1088
Li G, Quiros CF (2002) Genetic analysis, expression and molecular characterization of BoGSL-ELONG, a major gene involved in the aliphatic glucosinolate pathway of Brassica species. Genetics 162:1937–1943
Li G, Quiros CF (2003) In planta side-chain glucosinolate modification in Arabidopsis by introduction of dioxygenase Brassica homolog BoGSL-ALK. Theor Appl Genet 106:1116–1121
Li G, Gao M, Yang B, Quiros CF (2003) Gene to gene alignment between the Brassica and Arabidopsis genomes by transcriptional mapping. Theor Appl Genet 107:168–180
Li F, Hasegawa Y, Saito M, Shirasawa S, Fukushima A, Ito T, Fujii H, Kishitani S, Kitashiba H, Nishio T (2011) Extensive chromosome homoeology among Brassiceae species were revealed by comparative genetic mapping with high-density EST-based SNP markers in radish (Raphanus sativus L.). DNA Res 18:401–411
Liu et al (2014) The Brassica oleracea genome reveals the asymmetrical evolution of polyploid genomes. Nat Commun 5:3930
Melchini A, Traka MH (2010) Biological profile of erucin: a new promising anticancer agent from cruciferous vegetables. Toxins 2:593–612
Millan S, Sampedro MC, Gallejones P, Castellon A, Ibargoitia ML, Goicolea MA, Barrio RJ (2009) Identification and quantification of glucosinolates in rapeseed using liquid chromatography-ion trap mass spectrometry. Anal Bioanal Chem 394:1661–1669
Mithen RF, Clarke J, Lister C, Dean C (1995) Genetics of aliphatic glucosinolates. III. Side chain structure of aliphatic glucosinolates in Arabidopsis thaliana. Heredity 74:210–215
Mithen RF, Dekker M, Verkerk R, Rabot S, Johnson LT (2000) The nutritional significance, biosynthesis and bioavailability of glucosinolates in human foods. J Sci Food Agric 80:967–984
Montaut S, Barillari J, Iori R, Rollin P (2010) Glucoraphasatin: chemistry, occurrence, and biological properties. Phytochemistry 71:6–12
Moon JK, Kim JR, Ahn YJ, Shibamoto T (2010) Analysis and anti-Helicobacter activity of sulforaphane and related compounds present in broccoli (Brassica oleracea L.) sprouts. J Agric Food Chem 58:6672–6677
Nishio T, Kusaba M, Watanabe M, Hinata K (1996) Registration of S alleles in Brassica campestris L by the restriction fragment sizes of SLGs. Theor Appl Genet 92:388–394
Ozawa Y, Kawakishi S, Uda Y, Maeda Y (1990a) Isolation and identification of a novel b-carboline derivative in salted radish roots, Raphanus sativus L. Agr Biol Chem 54:1241–1245
Ozawa Y, Uda Y, Kawakishi S (1990b) Generation of b-carboline derivative, the yellowish precursor of processed radish roots, from 4-methylthio-3-butenyl isothiocyanate and L-tryptophan. Agr Biol Chem 54:1849–1851
Pedras MS, Chumala PB, Suchy M (2003) Phytoalexins from Thlaspi arvense, a wild crucifer resistant to virulent Leptosphaeria maculans: structures, syntheses and antifungal activity. Phytochemistry 64:949–956
Rosa EAS, Heaney RK, Fenwick GR, Portas CAM (1997) Glucosinolates in crop plants. Hortic Rev 19:99–125
Shiokai S, Kitashiba H, Nishio T (2010a) Prediction of the optimum hybridization conditions of dot-blot-SNP analysis using estimated melting temperature of oligonucleotide probes. Plant Cell Rep 29:829–834
Shiokai S, Shirasawa K, Sato Y, Nishio T (2010b) Improvement of the dot-blot-SNP technique for efficient and cost-effective genotyping. Mol Breed 25:179–185
Shirasawa K, Shiokai S, Yamaguchi M, Kishitani S, Nishio T (2006) Dot-blot-SNP analysis for practical plant breeding and cultivar identification in rice. Theor Appl Genet 113:147–155
Sønderby IE, Geu-Flores F, Halkier BA (2010) Biosynthesis of glucosinolates-gene discovery and beyond. Trends Plant Sci 15:283–290
Takahashi A, Yamada T, Uchiyama Y, Hayashi S, Kumakura K, Takahashi H, Kimura H, Matsuoka H (2015) Generation of the antioxidant yellow pigment derived from 4-methylthio-3-butenyl isothiocyanate in salted radish roots (takuan-zuke). Biosci Biotech Biochem. doi:10.1080/09168451.2015.1032881
Uda Y, Matsuoka H, Kumagai H, Maeda Y (1993) Stability and antimicrobial property of 4-methylthio-3-butenyl isothiocyanate, the pungent principle in radish. Nippon Shokuhin Kogyo Gakkaishi 40:743–746
Wang H, Wu J, Sun S, Liu B, Cheng F, Sun R, Wang X (2011) Glucosinolate biosynthetic genes in Brassica rapa. Gene 487:135–142
Wang Y, Pan Y, Liu Z, Zhu X, Zhai L, Xu L, Yu R, Gong Y, Liu L (2013) De novo transcriptome sequencing of radish (Raphanus sativus L.) and analysis of major genes involved in glucosinolate metabolism. BMC Genom 14:836
Zasada IA, Ferris H (2004) Nematode suppression with brassicaceous amendments: application based upon glucosinolate profiles. Soil Biology and Biochemistry 36:1017–1024
Zou Z, Ishida M, Li F, Kakizaki T, Suzuki S, Kitashiba H, Nishio T (2013) QTL analysis using SNP markers developed by next-generation sequencing for identification of candidate genes controlling 4-methylthio-3-butenyl glucosinolate contents in roots of radish, Raphanus sativus L. PLoS One 8:e53541
This work was partly supported by the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN), Japan.
Conflict of interest
The authors declare that they have no conflict of interest.
M. Ishida and T. Kakizaki contributed equally to this work.
Communicated by C. F. Quiros.
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Ishida, M., Kakizaki, T., Morimitsu, Y. et al. Novel glucosinolate composition lacking 4-methylthio-3-butenyl glucosinolate in Japanese white radish (Raphanus sativus L.). Theor Appl Genet 128, 2037–2046 (2015). https://doi.org/10.1007/s00122-015-2564-3
- BC1F1 Population
- White Radish
- Aliphatic GSLs