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Genetic analysis of bolting after winter in sugar beet (Beta vulgaris L.)

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Abstract

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This study reveals for the first time a major QTL for post-winter bolting resistance in sugar beet ( Beta vulgaris L.). The knowledge of this QTL is a major contribution towards the development of a winter sugar beet with controlled bolting behavior.

Abstract

In cool temperate climates, sugar beets are currently grown as a spring crop. They are sown in spring and harvested in autumn. Growing sugar beet as a winter crop with an extended vegetation period fails due to bolting after winter. Bolting after winter might be controlled by accumulating genes for post-winter bolting resistance. Previously, we had observed in field experiments a low post-winter bolting rate of 0.5 for sugar beet accession BETA 1773. This accession was crossed with a biennial sugar beet with regular bolting behavior to develop a F3 mapping population. The population was grown in the greenhouse, exposed to artificial cold treatment for 16 weeks and transplanted to the field. Bolting was recorded twice a week from May until October. Post-winter bolting behavior was assessed by two different factors, bolting delay (determined as days to bolt after cold treatment) and post-winter bolting resistance (bolting rate after winter). For days to bolt, means of F3 families ranged from 25 to 164 days while for bolting rate F3 families ranged from 0 to 1. For each factor one QTL explaining about 65 % of the phenotypic variation was mapped to the same region on linkage group 9 with a partially recessive allele increasing bolting delay and post-winter bolting resistance. The results are discussed in relation to the potential use of marker-assisted breeding of winter sugar beets with controlled bolting.

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Acknowledgments

This study was funded by the German Federal Ministry of Education and Research (BMBF) under the grant no. FKZ 0315465B. We thank Martin Kirchhoff, Meike Friedrichsen, Sabrina Butze, Monika Bruisch and Erwin Danklefsen for support and technical assistance in the lab, greenhouse and field. The Institute for Clinical Molecular Biology (IKMB), University Kiel, Germany, is acknowledged for collaborating in DNA sequencing.

Conflict of interest

None of the authors have any conflicts of interest associated with this study.

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Author notes

  1. Andreas E. Müller

    Present address: Strube Research GmbH & Co. KG, Hauptstr. 1, 38387, Söllingen, Germany

  2. Friedrich J. Kopisch-Obuch

    Present address: KWS SAAT AG, Grimsehlstrasse 31, 37555, Einbeck, Germany

Authors and Affiliations

  1. Plant Breeding Institute, Christian Albrechts University of Kiel, Olshausenstrasse 40, 24118, Kiel, Germany

    Nina Pfeiffer, Conny Tränkner, Andreas E. Müller, Christian Jung & Friedrich J. Kopisch-Obuch

  2. Institute of Computer Science, Martin-Luther-University Halle-Wittenberg, Von-Seckendorff-Platz 1, 06120, Halle, Germany

    Ioana Lemnian & Ivo Grosse

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  1. Nina Pfeiffer
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Correspondence to Friedrich J. Kopisch-Obuch.

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Communicated by Heiko C. Becker.

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Pfeiffer, N., Tränkner, C., Lemnian, I. et al. Genetic analysis of bolting after winter in sugar beet (Beta vulgaris L.). Theor Appl Genet 127, 2479–2489 (2014). https://doi.org/10.1007/s00122-014-2392-x

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