Neijiang 977671 and 19 near-isogenic lines with known leaf rust resistance genes were inoculated with 12 pathotypes of Puccinia triticina for postulation of leaf rust resistance genes effective at the seedling stage. The reaction pattern of Neijiang 977671 differed from those of the lines with known leaf rust resistance genes used in the test, indicating that Neijiang 977671 may carry a new leaf rust resistance gene(s). With the objective of identifying and mapping the new gene for resistance to leaf rust, F1 and F2 plants, and F2:3 families, from Neijiang 977671 × Zhengzhou 5389 (susceptible) were inoculated with Chinese P. triticina pathotype FHNQ in the greenhouse. Results from the F2 and F2:3 populations indicated that a single dominant gene, temporarily designated LrNJ97, conferred resistance. In order to identify other possible genes in Neijiang 977671 other eight P. triticina pathotypes avirulent on Neijiang 977671 were used to inoculate 25 F2:3 families. The results showed that at least three leaf rust resistance genes were deduced in Neijiang 977671. Bulked segregant analysis was performed on equal amounts of genomic DNA from 20 resistant and 20 susceptible F2 plants. SSR markers polymorphic between the resistant and susceptible bulks were used to analyze the F2:3 families. LrNJ97 was linked to five SSR loci on chromosome 2BL. The two closest flanking SSR loci were Xwmc317 and Xbarc159 at genetic distances of 4.2 and 2.2 cM, respectively. At present two designated genes (Lr50 and Lr58) are located on chromosome 2BL. In the seedling tests, the reaction pattern of LrNJ97 was different from that of Lr50. Lr50 and Lr58 were derived from T. armeniacum and Ae. triuncialis, respectively, whereas according to the pedigree of Neijiang 977671 LrNJ97 is from common wheat. Although seeds of lines with Lr58 were not available, it was concluded that LrNJ97 is likely to be a new leaf rust resistance gene.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Bassam BJ, Caetano-Anolles G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80–83
Bolton MD, Kolmer JA, Garvin DF (2008) Wheat leaf rust caused by Puccinia triticina. Mol Plant Pathol 9:563–575
Brown-Guerdira GL, Singh S, Fritz AK (2003) Performance and mapping of leaf rust resistance to wheat from Triticum timopheevii subsp. ameniacum. Phytopathology 93:784–789
Bryan GJ, Collins AJ, Stephenson P, Orry A, Smith JB, Gale MD (1997) Isolation and characterization of microsatellites from hexaploid bread wheat. Theor Appl Genet 94:557–563
Dong JG (ed) (2001) Agricultural plant pathology. China Agriculture Press, Beijing (In Chinese)
Eriksen L, Afshari F, Christiansen MJ, McIntosh RA, Jahoor A, Wellings CR (2004) Yr32 for resistance to stripe rust present in the wheat cultivar Carstens V. Theor Appl Genet 108:567–575
Helguera M, Vanzetti L, Soria M, Khan IA, Kolmer J, Dubcovsky J (2005) PCR markers for Triticum speltoides leaf rust resistance gene Lr51 and their use to develop isogenic hard red spring wheat lines. Crop Sci 45:728–734
Herrera-Foessel SA, Singh RP, Huerta-Espino J, Rosewarne GM, Periyannan SK, Viccars L, Calvo-Salazar V, Lan C, Lagudah ES (2012) Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat. Theor Appl Genet 124:1475–1486
Hiebert C, Thomas J, McCallum B (2005) Locating the broad-spectrum wheat leaf rust resistance gene Lr52 by a new cytogenetic method. Theor Appl Genet 111:1453–1457
Khan RR, Bariana HS, Dholakia BB, Naik SV, Lagu MD, Rathjen AJ, Bhavani S, Gupta VS (2005) Molecular mapping of stem and leaf rust resistance in wheat. Theor Appl Genet 111:846–850
Kosambi DD (1944) The estimation of map distances from recombination values. Annu Eugen 12:172–175
Kuraparthy V, Sood S, Chhuneja P, Dhaliwal HS, Laur S, Bowden RL, Gill BS (2007) A cryptic wheat-Aegilops triuncialis translocation with leaf rust resistance gene Lr58. Crop Sci 47:1995–2003
Li ZF, Xia XC, He ZH, Li X, Zhang LJ, Wang HY, Meng QF, Yang WX, Li GQ, Liu DQ (2010) Seedling and slow rusting resistance to leaf rust in Chinese wheat cultivars. Plant Dis 94:45–53
Long DL, Kolmer JA (1989) A North American system of nomenclature for Puccinia recondita f. sp. tritici. Phytopathology 79:525–529
Manly FF, Cudmore RH, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932
McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2009) Catalogue of gene symbols for wheat: 2009 supplement. Komugi integrated wheat science database. http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2009.pdf
McIntosh RA, Dubcovsky J, Rogers WJ, Morris CF, Appels R, Xia XC (2011) Catalogue of gene symbols for wheat: 2011 supplement. Ann Wheat News Lett 57:303–321
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity of leaves and stems of cereals. Can J Res 26:496–500
Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Roelfs AP, Singh RP, Saari EE (1992) Rust diseases of wheat: concepts and methods of disease management. CIMMYT, Mexico, D.F.
Sacco F, Suarez EY, Narango T (1988) Mapping of the leaf rust resistance gene Lr3 on chromosome 6B of Sinvalocho MA wheat. Genome 41:686–690
Sharp PJ, Kreis M, Shewry PR, Gale MD (1988) Location of β-amylase sequence in wheat and its relatives. Theor Appl Genet 75:286–290
Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114
Sourdille P, Guyomarc’h H, Baron C, Gandon B, Chiquet V, Artiguenave F, Edwards K, Foisset N, Dufour P (2001) Improvement of the genetic maps of wheat using new microsatellite markers. Plant animal genome IX abstracts, pp 167
Watson IA, Singh D (1952) The future for rust resistant wheat in Australia. J Aust Inst Agric Sci 18:190–197
Yang WX, Liu DQ (2004) Advances in localization and molecular markers of wheat leaf rust resistance genes. Sci Agric Sinica 37(1):65–71 (In Chinese)
Zhang H, Xia XC, He ZH, Li X, Li ZF, Liu DQ (2011) Molecular mapping of leaf rust resistance gene LrBi16 in Chinese wheat cultivar Bimai 16. Mol Breed 28:527–534
Zhao XL, Zheng TC, Xia XC, He ZH, Liu DQ, Yang WX, Yin GH, Li ZF (2008) Molecular mapping of leaf rust resistance gene LrZH84 in Chinese wheat line Zhou 8425B. Theor Appl Genet 117:1069–1075
We are grateful to the critical review of this manuscript by Prof. R. A. McIntosh, Plant Breeding Institute, University of Sydney, Australia. The project was supported by National Natural Science Foundation of China (30971772) and Postdoctoral Science Foundation of China (2011M500672).
Communicated by S. Dreisigacker.
About this article
Cite this article
Zhou, H., Xia, X., He, Z. et al. Molecular mapping of leaf rust resistance gene LrNJ97 in Chinese wheat line Neijiang 977671. Theor Appl Genet 126, 2141–2147 (2013). https://doi.org/10.1007/s00122-013-2124-7
- Simple Sequence Repeat Marker
- Leaf Rust
- Simple Sequence Repeat Locus
- Leaf Rust Resistance
- Leaf Rust Resistance Gene