Skip to main content
SpringerLink
Log in

QTL mapping of adult-plant resistances to stripe rust and leaf rust in Chinese wheat cultivar Bainong 64

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Stripe rust and leaf rust, caused by Puccinia striiformis Westend. f. sp. tritici Erikss. and P. triticina, respectively, are devastating fungal diseases of common wheat (Triticum aestivum L.). Chinese wheat cultivar Bainong 64 has maintained acceptable adult-plant resistance (APR) to stripe rust, leaf rust and powdery mildew for more than 10 years. The aim of this study was to identify quantitative trait loci/locus (QTL) for resistance to the two rusts in a population of 179 doubled haploid (DH) lines derived from Bainong 64 × Jingshuang 16. The DH lines were planted in randomized complete blocks with three replicates at four locations. Stripe rust tests were conducted using a mixture of currently prevalent P. striiformis races, and leaf rust tests were performed with P. triticina race THTT. Leaf rust severities were scored two or three times, whereas maximum disease severities (MDS) were recorded for stripe rust. Using bulked segregant analysis (BSA) and simple sequence repeat (SSR) markers, five independent loci for APR to two rusts were detected. The QTL on chromosomes 1BL and 6BS contributed by Bainong 64 conferred resistance to both diseases. The loci identified on chromosomes 7AS and 4DL had minor effects on stripe rust response, whereas another locus, close to the centromere on chromosome 6BS, had a significant effect only on leaf rust response. The loci located on chromosomes 1BL and 4DL also had significant effects on powdery mildew response. These were located at the same positions as the Yr29/Lr46 and Yr46/Lr67 genes, respectively. The multiple disease resistance locus for APR on chromosome 6BS appears to be new. All three genes and their closely linked molecular markers could be used in breeding wheat cultivars with durable resistance to multiple diseases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Afzal SN, Haque MI, Ahmedani MS, Bashir S, Rattu AUR (2007) Assessment of yield losses caused by Puccinia striiformis triggering stripe rust in the most common wheat varieties. Pak J Bot 39:2127–2134

    Google Scholar 

  • Allard RW (1960) Princilpes of Plant Breeding. John Wiley and Sons, Inc., New York

    Google Scholar 

  • Bhavani S, Singh RP, Argillier O, Huerta-Espino J, Singh S, Njau P, Brun S, Lacam S, Desmouceaux N (2011) Mapping durable adult plant stem rust resistance to the race Ug99 group in six CIMMYT wheats. 2011 BGRI Technical Workshop, June 13–16, University of Minnesota and USDA Cereal Disease Lab, St. Paul, Minnesota. http://www.globalrust.org/db/attachments/knowledge/122/2/Bhavani-revised.pdf

  • Bjarko ME, Line RF (1988) Heritability and number of genes controlling leaf rust resistance on four cultivars of wheat. Phytopathology 78:457–461

    Article  Google Scholar 

  • Caldwell RM (1968) Breeding for general and/or specific plant disease resistance. In: Findlay KW, Shepherd KW (eds) Proc 3rd Int Wheat Genetics Symp Australian Academy of Science, Canberra, pp 263–272

    Google Scholar 

  • Carter AH, Chen XM, Garland-Campbell K, Kidwell KK (2009) Identifying QTL for high-temperature adult-plant resistance to stripe rust (Puccinia striiformis f. sp. tritici) in the spring wheat (Triticum aestivum L.) cultivar ‘Louise’. Theor Appl Genet 119:1119–1128

    Article  PubMed  Google Scholar 

  • Chen XM (2005) Epidemiology and control of stripe rust [Puccinia striiformis f. sp. tritici] on wheat. Can J Plant Pathol 27:314–337

    Article  Google Scholar 

  • Chen XM, Line RF (1995a) Gene action in wheat cultivars for durable high-temperature adult-plant resistance and interactions with race-specific, seedling resistance to stripe rust caused by Puccinia striiformis. Phytopathology 85:567–572

    Article  Google Scholar 

  • Chen XM, Line RF (1995b) Gene number and heritability of wheat cultivars with durable, high-temperature, adult-plant resistance and race-specific resistance to Puccinia striiformis. Phytopathology 85:573–578

    Article  Google Scholar 

  • Dedryver F, Paillard S, Mallard S, Robert O, Trottet M, Nègre S, Verplancke G, Jahier J (2009) Characterization of genetic components involved in durable resistance to stripe rust in the bread wheat ‘Renan’. Phytopathology 99:968–973

    Article  PubMed  CAS  Google Scholar 

  • Fu DL, Uauy C, Distelfeld A, Blechl A, Epstein L, Chen XM, Sela H, Fahima T, Dubcovsky J (2009) A kinase-start gene confers temperature-dependent resistance to wheat stripe rust. Science 323:1357–1360

    Article  PubMed  CAS  Google Scholar 

  • Gupta PK, Balyan HS, Edwards KJ, Isaac P, Korzun V, Röder M, Gautier M-F, Joudrier P, Schlatter AR, Dubcovsky J, De la Pena RC, Khairallah M, Penner G, Hayden MJ, Sharp P, Keller B, Wang RCC, Hardouin JP, Jack P, Leroy P (2002) Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor Appl Genet 105:413–422

    Article  PubMed  CAS  Google Scholar 

  • He ZH, Lan CX, Chen XM, Zou YC, Zhuang QS, Xia XC (2011) Progress and perspective in research of adult-plant resistance to stripe rust and powdery mildew in wheat. Sci Agric Sin 44:2193–2215

    Google Scholar 

  • Herrera-Foessel SA, Lagudah ES, Huerta-Espino J, Hayden MJ, Bariana HS, Singh D, Singh RP (2011) New slow-rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked. Theor Appl Genet 122:239–249

    Article  PubMed  Google Scholar 

  • Herrera-Foessel SA, Singh RP, Huerta-Espino J, Rosewarne GM, Periyannan SK, Viccars L, Calvo-Salazar V, Lan CX, Lagudah ES (2012) Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat. Theor Appl Genet 124:1475–1486

    Article  PubMed  CAS  Google Scholar 

  • Hiebert CW, Thomas JB, McCallum BD, Humphreys DG, DePauw RM, Hayden MJ, Mago R, Schnippenkoetter W, Spielmeyer W (2010) An introgression on wheat chromosome 4DL in RL6077 (Thatcher*6/PI 250413) confers adult plant resistance to stripe rust and leaf rust (Lr67). Theor Appl Genet 121:1083–1091

    Article  PubMed  Google Scholar 

  • Kilpatrick RA (1975) New wheat cultivars and longevity of rust resistance, 1971–1975. US Department of Agriculture, Agricultural Research Service, Beltsville

    Google Scholar 

  • Knott DR (1989) The Wheat Rusts-Breeding for Resistance. In: Monographs on Theoretical and Applied Genetics, vol 12. Springer, Verlag, Berlin, pp 201

  • Kosambi DD (1944) The estimation of map distance from recombination values. Annu Eugen 12:172–175

    Google Scholar 

  • Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1363

    Article  PubMed  CAS  Google Scholar 

  • Lagudah ES, McFadden H, Singh RP, Huerta-Espino J, Bariana HS, Spielmeyer W (2006) Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor Appl Genet 114:21–30

    Article  PubMed  CAS  Google Scholar 

  • Lagudah ES, Krattinger SG, Herrera-Foessel S, Singh RP, Huerta-Espino J, Spielmeyer W, Brown-Guedira G, Selter LL, Keller B (2009) Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theor Appl Genet 119:889–898

    Article  PubMed  CAS  Google Scholar 

  • Lan CX, Liang SS, Wang ZL, Yan J, Zhang Y, Xia XC, He ZH (2009) Quantitative trait loci mapping for adult-plant resistance to powdery mildew in Chinese wheat cultivar Bainong 64. Phytopathology 99:1121–1126

    Article  PubMed  Google Scholar 

  • Lan CX, Liang SS, Zhou XC, Zhou G, Lu QL, Xia XC, He ZH (2010) Identification of genomic regions controlling adult-plant stripe rust resistance in Chinese landrace Pingyuan 50 through bulked segregant analysis. Phytopathology 100:313–318

    Article  PubMed  Google Scholar 

  • Li HH, Ribaut J-M, Li ZL, Wang JK (2008) Inclusive composite interval mapping (ICIM) for digenic epistasis of quantitative traits in biparental populations. Theor Appl Genet 116:243–260

    Article  PubMed  Google Scholar 

  • Li ZF, Xia XC, He ZH, Li X, Zhang LJ, Wang HY, Meng QF, Yang WX, Li GQ, Liu DQ (2010) Seedling and slow rusting resistance to leaf rust in Chinese wheat cultivars. Plant Dis 94:45–53

    Article  CAS  Google Scholar 

  • Liang SS, Suenaga K, He ZH, Wang ZL, Liu HY, Wang DS, Singh RP, Sourdille P, Xia XC (2006) Quantitative trait loci mapping for adult-plant resistance to powdery mildew in bread wheat. Phytopathology 96:784–789

    Article  PubMed  CAS  Google Scholar 

  • Lillemo M, Asalf B, Singh RP, Huerta-Espino J, Chen XM, He ZH, Bjørnstad Å (2008) The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor Appl Genet 116:1155–1166

    Article  PubMed  CAS  Google Scholar 

  • Line RF (2002) Stripe rust of wheat and barley in North America: a retrospective historical review. Annu Rev Phytopathol 40:75–118

    Article  PubMed  CAS  Google Scholar 

  • Line RF, Chen XM (1995) Success in breeding for and managing durable resistance to wheat rusts. Plant Dis 79:1254–1255

    Google Scholar 

  • Lu YM, Lan CX, Liang SS, Zhou XC, Liu D, Zhou G, Lu QL, Jing JX, Wang MN, Xia XC, He ZH (2009) QTL mapping for adult-plant resistance to stripe rust in Italian common wheat cultivars Libellula and Strampelli. Theor Appl Genet 119:1349–1359

    Article  PubMed  CAS  Google Scholar 

  • Manly KF, Cudmore RH Jr, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Genome 12:930–932

    CAS  Google Scholar 

  • McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2011) Catalogue of gene symbols for wheat: 2011 supplement. http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2011.pdf

  • McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2012) Catalogue of gene symbols for wheat: 2012 supplement. http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2012.pdf

  • Pestsova E, Ganal MW, Röder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697

    Article  PubMed  CAS  Google Scholar 

  • Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity of leaves and stems of cereals. Can J Res 26:496–500

    Article  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Rosewarne GM, Singh RP, Huerta-Espino J, William HM, Bouchet S, Cloutier S, McFadden H, Lagudah ES (2006) Leaf tip necrosis, molecular markers and β1-proteasome subunits associated with the slow rusting resistance genes Lr46/Yr29. Theor Appl Genet 112:500–508

    Article  PubMed  CAS  Google Scholar 

  • Samborski DJ (1985) Wheat Leaf Rust. In: Roelfs AP, Bushnell WR (eds) The cereal rusts, vol 2. Academic Press, Orlando, Fla., pp 39–59

    Google Scholar 

  • Santra DK, Chen XM, Santra M, Campbell KG, Kidwell KK (2008) Identification and mapping QTL for high-temperature adult-plant resistance to stripe rust in winter wheat (Triticum aestivum L.) cultivar ‘Stephens’. Theor Appl Genet 117:793–802

    Article  PubMed  CAS  Google Scholar 

  • Singh RP (1992) Association between gene Lr34 for leaf rust resistance and leaf tip necrosis in wheat. Crop Sci 32:874–878

    Article  Google Scholar 

  • Singh RP, Huerta-Espino J, Rajaram S (2000) Achieving near immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathologica et Entomologica Hungarica 35:133–139

    CAS  Google Scholar 

  • Singh RP, Huerta-Espino J, William HM (2005) Genetics and breeding for durable resistance to leaf and stripe rusts in wheat. Turk J Agric For 29:121–127

    CAS  Google Scholar 

  • Singh RP, Huerta-Espino J, Bhavani S, Herrera-Foessel SA, Singh D, Singh PK, Velu G, Mason RE, Jin Y, Njau P, Crossa J (2011) Race non-specific resistance to rust diseases in CIMMYT spring wheats. Euphytica 179:175–186

    Article  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  PubMed  CAS  Google Scholar 

  • Song QJ, Fickus EW, Cregan PB (2002) Characterization of trinucleotide SSR motifs in wheat. Theor Appl Genet 104:286–293

    Article  PubMed  CAS  Google Scholar 

  • Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi L, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genomics 4:12–25

    Article  PubMed  CAS  Google Scholar 

  • Suenaga K, Singh RP, Huerta-Espino J, William HM (2003) Microsatellite markers for genes Lr34/Yr18 and other quantitative trait loci for leaf rust and stripe rust resistance in bread wheat. Phytopathology 93:881–890

    Article  PubMed  CAS  Google Scholar 

  • Wang S, Basten CJ, Zeng ZB (2005a) Windows QTL cartographer v2.5 statistical genetics. North Carolina State University, Raleigh, NC

  • Wang ZL, Li LH, He ZH, Duan XY, Zhou YL, Chen XM, Lillemo M, Singh RP, Wang H, Xia XC (2005b) Seeding and adult-plant resistance to powdery mildew in Chinese bread wheat cultivars and lines. Plant Dis 89:457–463

    Article  CAS  Google Scholar 

  • Wang ZL, Liu SD, Liu HY, He ZH, Xia XC, Chen XM (2006) Genetic linkage map in Bainong 64 × Jingshuang 16 of wheat. Acta Bot Boreal Occident Sin 26:886–892

    CAS  Google Scholar 

  • William M, Singh RP, Huerta-Espino J, Ortiz Islas S, Hoisington D (2003) Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat. Phytopathology 93:153–159

    Article  PubMed  CAS  Google Scholar 

  • William HM, Singh RP, Huerta-Espino J, Palacios G, Suenaga K (2006) Characterization of genetic loci conferring adult plant resistance to leaf rust and stripe rust in spring wheat. Genome 49:977–990

    Article  PubMed  CAS  Google Scholar 

  • Yang ZM, Xie CJ, Sun QX (2003) Situation of the sources of stripe rust resistance of wheat in the post-CYR32 era in China. Acta Agron Sin 29:161–168

    Google Scholar 

  • Yuan JH, Liu TG, Chen WQ (2007) Postulation of leaf rust resistance genes in 47 new wheat cultivars at seedling stage. Sci Agric Sin 40:1925–1935

    CAS  Google Scholar 

  • Zhang LJ, Li ZF, Lillemo M, Xia XC, Liu DQ, Yang WX, Luo JC, Wang HY (2009) QTL mapping for adult-plant resistance to leaf rust in CIMMYT wheat cultivar Saar. Sci Agric Sin 42:388–397

    CAS  Google Scholar 

  • Zhao XL, Zheng TC, Xia XC, He ZH, Liu DQ, Yang WX, Yin GH, Li ZF (2008) Molecular mapping of leaf rust resistance gene LrZH84 in Chinese wheat line Zhou 8425B. Theor Appl Genet 117:1069–1075

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We are grateful to the critical review of this manuscript by Prof. R. A. McIntosh, Plant Breeding Institute, University of Sydney, Australia. This study was supported by the China Agriculture Research System (CARS-3-1-3), International Collaboration Project from the Ministry of Agriculture (2011-G3), National 863 project (2012AA101105) and National Natural Science Foundation of China (30821140351).

Author information

Authors and Affiliations

  1. Institute of Crop Science, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China

    Yan Ren, Zhonghu He, Bin Bai, Caixia Lan & Xianchun Xia

  2. Department of Plant Pathology, College of Plant Protection, Agricultural University of Hebei, Biological Control Center for Plant Diseases and Plant Pests of Hebei, 289 Lingyusi Street, Baoding, 071001, Hebei, China

    Zaifeng Li & Cuifen Wang

  3. Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China

    Ling Wu & Huazhong Zhu

  4. Wheat Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, Gansu, China

    Bin Bai & Gang Zhou

  5. International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing, 100081, China

    Zhonghu He

Authors
  1. Yan Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zaifeng Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhonghu He
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ling Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bin Bai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Caixia Lan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Cuifen Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Gang Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Huazhong Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Xianchun Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xianchun Xia.

Additional information

Communicated by M. Sorrells.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 247 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ren, Y., Li, Z., He, Z. et al. QTL mapping of adult-plant resistances to stripe rust and leaf rust in Chinese wheat cultivar Bainong 64. Theor Appl Genet 125, 1253–1262 (2012). https://doi.org/10.1007/s00122-012-1910-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-012-1910-y

Keywords

Search

Navigation