Advertisement

Theoretical and Applied Genetics

, Volume 122, Issue 1, pp 129–142 | Cite as

QTL mapping of internal heat necrosis in tetraploid potato

  • P. H. McCord
  • B. R. Sosinski
  • K. G. Haynes
  • M. E. Clough
  • G. C. YenchoEmail author
Original Paper

Abstract

Internal heat necrosis (IHN) is a physiological disorder of potato tubers. We developed a linkage map of tetraploid potato using AFLP and SSR markers, and mapped QTL for mean severity and percent incidence of IHN. Phenotypic data indicated that the distribution of IHN is skewed toward resistance. Late foliage maturity was slightly but significantly correlated with increased IHN symptoms. The linkage map for ‘Atlantic’, the IHN-susceptible parent, covered 1034.4 cM and included 13 linkage groups, and the map for B1829-5, the IHN-resistant parent, covered 940.2 cM and contained 14 linkage groups. QTL for increased resistance to IHN were located on chromosomes IV, V, and groups VII and X of ‘Atlantic’, and on group VII of B1829-5 in at least 2 of 3 years. The QTL explained between 4.5 and 29.4% of the variation for mean severity, and from 3.7 to 14.5% of the variation for percent incidence. Most QTL detected were dominant, and associated with decreased IHN symptoms. One SSR and 13 AFLP markers that were linked to IHN were tested in a second population. One AFLP marker was associated with decreased symptoms in both populations. The SSR marker was not associated with IHN in the second population, but was closely linked in repulsion to another marker that was associated with IHN, and had the same (negative) effect on the trait as the SSR marker did in the first population. The correlation between maturity and IHN may be partially explained by the presence of markers on chromosome V that are linked to both traits. This research represents the first molecular genetic research of IHN in potato.

Keywords

QTL Internal heat necrosis Linkage mapping Potato Tetraploid Solanum tuberosum 

Supplementary material

122_2010_1429_MOESM1_ESM.tiff (553 kb)
Supplementary material 1 (TIFF 552 kb)
122_2010_1429_MOESM2_ESM.tiff (839 kb)
Supplementary material 2 (TIFF 839 kb)
122_2010_1429_MOESM3_ESM.tiff (559 kb)
Supplementary material 3 (TIFF 558 kb)
122_2010_1429_MOESM4_ESM.tiff (478 kb)
Supplementary material 4 (TIFF 477 kb)
122_2010_1429_MOESM5_ESM.tiff (827 kb)
Supplementary material 5 (TIFF 826 kb)
122_2010_1429_MOESM6_ESM.tiff (412 kb)
Supplementary material 6 (TIFF 412 kb)
122_2010_1429_MOESM7_ESM.tiff (1.1 mb)
Supplementary material 7 (TIFF 1128 kb)
122_2010_1429_MOESM8_ESM.tiff (214 kb)
Supplementary material 8 (TIFF 213 kb)
122_2010_1429_MOESM9_ESM.tiff (310 kb)
Supplementary material 9 (TIFF 310 kb)
122_2010_1429_MOESM10_ESM.tiff (591 kb)
Supplementary material 10 (TIFF 590 kb)
122_2010_1429_MOESM11_ESM.tiff (698 kb)
Supplementary material 11 (TIFF 698 kb)
122_2010_1429_MOESM12_ESM.tiff (351 kb)
Supplementary material 12 (TIFF 351 kb)
122_2010_1429_MOESM13_ESM.doc (40 kb)
Supplementary material 13 (DOC 40 kb)
122_2010_1429_MOESM14_ESM.doc (22 kb)
Supplementary material 14 (DOC 22 kb)

References

  1. Anonymous (2008) United States Department of Agriculture, Agricultural Marketing Service. United States standards for grades of potatoesGoogle Scholar
  2. Asins MJ, Bernet GP, Ruiz C, Cambra M, Guerri J, Carbonell EA (2004) QTL analysis of citrus tristeza virus–citradia interaction. Theor Appl Genet 108:603–611CrossRefPubMedGoogle Scholar
  3. Bradshaw HD Jr, Wilbert SM, Otto KG, Schemske DW (1995) Genetic mapping of floral traits associated with reproductive isolation in monkeyflowers (Mimulus). Nature 376:762–765CrossRefGoogle Scholar
  4. Bradshaw JE, Pande B, Bryan GJ, Hackett CA, McLean K, Stewart HE, Waugh R (2004) Interval mapping of quantitative trait loci for resistance to late blight [Phytophthora infestans (Mont.) de Bary], height and maturity in a tetraploid population of potato (Solanum tuberosum subsp. tuberosum). Genetics 168:983–995CrossRefPubMedGoogle Scholar
  5. Bradshaw JE, Hackett CA, Pande B, Waugh R, Bryan GJ (2008) QTL mapping of yield, agronomic and quality traits in tetraploid potato (Solanum tuberosum subsp. tuberosum). Theor Appl Genet 116:193–211CrossRefPubMedGoogle Scholar
  6. Buntjer J (2000) Cross checker: computer assisted scoring of AFLP data. In: Plant and animal genome conference VIII, San Diego CA, 9–12 January 2000. Software download from http://www.plantbreeding.wur.nl/UK/software_crosschecker_download2.html. Accessed 23 November 2009
  7. Cho S, Chen W, Muehlbauer FJ (2004) Pathotype-specific genetic factors in chickpea (Cicer arietinum L.) for quantitative resistance to ascochyta blight. Theor Appl Genet 109:733–739CrossRefPubMedGoogle Scholar
  8. Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971PubMedGoogle Scholar
  9. Davies HV (1998) Physiological mechanisms associated with the development of internal necrotic disorders of potato. Am J Potato Res 75:37–44CrossRefGoogle Scholar
  10. Douches D (2010) SOLCAP Newsl 2:1-2. http://solcap.msu.edu/pdf%20files/SolcapnewsMarch10.pdf
  11. Feingold S, Lloyd J, Norero N, Bonierbale M, Lorenzen J (2005) Mapping and characterization of new EST-derived microsatellites for potato (Solanum tuberosum L.). Theor Appl Genet 111:456–466CrossRefPubMedGoogle Scholar
  12. Frary A, Xu Y, Liu J, Mitchell S, Tedeschi E, Tanksley S (2005) Development of a set of PCR-based anchor markers encompassing the tomato genome and evaluation of their usefulness for genetics and breeding experiments. Theor Appl Genet 111:291–312CrossRefPubMedGoogle Scholar
  13. Hackett CA, Bradshaw JE, McNicol JW (2001) Interval mapping of quantitative trait loci in autotetraploid species. Genetics 159:1819–1832PubMedGoogle Scholar
  14. Hackett CA, Pande B, Bryan GJ (2003) Constructing linkage maps in autotetraploid species using simulated annealing. Theor Appl Genet 106:1107–1115PubMedGoogle Scholar
  15. Hackett CA, Milne I, Bradshaw JE, Luo Z (2007) TetraploidMap for Windows: linkage map construction and QTL mapping in autotetraploid species. J Hered 98:727–729CrossRefPubMedGoogle Scholar
  16. Henninger MR, Patterson JW, Webb RE (1979) Tuber necrosis in ‘Atlantic’. Am J Potato Res 56:464Google Scholar
  17. Henninger MR, Sterrett SB, Haynes KG (2000) Broad-sense heritability and stability of internal heat necrosis and specific gravity in tetraploid potatoes. Crop Sci 40:977–984CrossRefGoogle Scholar
  18. Joehanes R, Nelson JC (2008) QGene 4.0, an extensible Java QTL-analysis platform. Bioinformatics 24:2788–2789CrossRefPubMedGoogle Scholar
  19. Julier B, Flajoulot S, Barre P, Cardinet G, Santoni S, Huguet T, Huyghe C (2003) Construction of two genetic linkage maps in cultivated tetraploid alfalfa (Medicago sativa) using microsatellite and AFLP markers. BMC Plant Biol 3:1–19CrossRefGoogle Scholar
  20. Kelley KB, Whitworth JL, Novy RG (2009) Mapping of the potato leafroll virus resistance gene, Rlr etb, from Solanum etuberosum identifies interchromosomal translocations among its E-genomes 4 and 9 relative to the A-genome of Solanum L. sect. Petota. Mol Breed 23:489–500CrossRefGoogle Scholar
  21. Khu D, Lorenzen J, Hackett CA, Love SL (2008) Interval mapping of quantitative trait loci for corky ringspot disease resistance in a tetraploid population of potato (Solanum tuberosum subsp. tuberosum). Am J Potato Res 85:129–139CrossRefGoogle Scholar
  22. Knox MR, Ellis THN (2001) Stability and inheritance of methylation states at PstI sites in Pisum. Mol Genet Genomics 265:497–507CrossRefPubMedGoogle Scholar
  23. Larson RH, Albert AR (1945) Physiological internal necrosis of potato tubers in Wisconsin. J Agric Res 71:487–504PubMedGoogle Scholar
  24. Luo ZW, Zhang RM, Kearsey MJ (2004) Theoretical basis for genetic linkage analysis in autotetraploid species. Proc Natl Acad Sci USA 101:7040–7045CrossRefPubMedGoogle Scholar
  25. McClelland M, Nelson M, Raschke E (1994) Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases. Nucleic Acid Res 22:3640–3659CrossRefPubMedGoogle Scholar
  26. McCord PH, Sosinski BR, Haynes KG, Clough ME, Yencho GC (2010) Linkage mapping and QTL analysis of agronomic traits in tetraploid potato (Solanum tuberosum subsp. tuberosum). Crop Sci (in press)Google Scholar
  27. Myburg AA, Remington DL (2000) Protocol for high-throughput AFLP analysis using Li-Cor IR2 automated sequencers. http://www.up.ac.za/academic/fabi/eucgenomics/euc_mapping/AFLP_protocol.pdf. Accessed 23 November 2009
  28. Rampling LR, Harker N, Shariflou MR, Morell MK (2001) Detection and analysis systems for microsatellite markers in wheat. Aust J Agric Res 52:1131–1141CrossRefGoogle Scholar
  29. Robins JG, Hansen JL, Viands DR, Brummer EC (2008) Genetic mapping of persistence in tetraploid alfalfa. Crop Sci 48:1780–1786CrossRefGoogle Scholar
  30. Sagredo B, Lafta A, Casper H, Lorenzen J (2006) Mapping of genes associated with leptine content in tetraploid potato. Theor Appl Genet 114:131–142CrossRefPubMedGoogle Scholar
  31. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234CrossRefPubMedGoogle Scholar
  32. Song K, Ping L, Tang K, Osborn TC (1995) Rapid genome change in synthetic polyploids of Brassica and its implications for polyploidy evolution. Proc Natl Acad Sci USA 92:7719–7723CrossRefPubMedGoogle Scholar
  33. Sterrett SB, Henninger MR (1997) Internal heat necrosis in the mid-Atlantic region—influence of environment and cultural management. Am J Potato Res 74:233–243CrossRefGoogle Scholar
  34. Sterrett SB, Wilson GL (1990) Internal heat necrosis in ‘Atlantic’: a survey of the disorder. Veg Grow News 44:2–4Google Scholar
  35. Sterrett SB, Henninger MR, Lee GS (1991) Relationship of internal heat necrosis of potato to time and temperature after planting. J Am Soc Hortic Sci 116:697–700Google Scholar
  36. Sterrett SB, Henninger MR, Yencho GC, Lu W, Vinyard BT, Haynes KG (2003) Stability of internal heat necrosis and specific gravity in tetraploid X diploid potatoes. Crop Sci 43:790–796CrossRefGoogle Scholar
  37. Tzeng KC, Kelman A, Simmons KE, Kelling KA (1986) Relationship of calcium nutrition to internal brown spot of potato tubers and sub-apical necrosis of sprouts. Am J Potato Res 63:87–97CrossRefGoogle Scholar
  38. van Os H, Andrzejewski S, Bakker E, Barrena I, Bryan GJ, Caromel B, Ghareeb B, Isidore E, De Jong W, van Koert P, Lefebvre V, Milbourne D, Ritter E, van der Voort JNAMR, Rousselle-Bourgeois F, van Vliet J, Waugh R, Visser RGF, Bakker J, van Eck HJ (2006) Construction of a 10,000-marker ultradense genetic recombination map of potato: providing a framework for accelerated gene isolation and a genome wide physical map. Genetics 173:1075–1087CrossRefPubMedGoogle Scholar
  39. Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78CrossRefPubMedGoogle Scholar
  40. Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Friters A, Pot J, Paleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acid Res 23:4407–4414CrossRefPubMedGoogle Scholar
  41. Wang S, Basten CJ, Zheng Z-B (2007) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC. http://statgen.ncsu.edu/qtlcart/WQTLCART.htm. Accessed 23 November 2009
  42. Webb RE, Wilson DR, Shumaker JR, Graves B, Henninger MR, Watts J, Frank JA, Murphy HJ (1978) Atlantic: a new potato variety with high solids, good processing quality and resistance to pests. Am J Potato Res 55:141–145CrossRefGoogle Scholar
  43. Yencho GC, McCord PH, Haynes KG, Sterrett SB (2008) Internal heat necrosis—a review. Am J Potato Res 85:69–76CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • P. H. McCord
    • 1
  • B. R. Sosinski
    • 2
  • K. G. Haynes
    • 3
  • M. E. Clough
    • 4
  • G. C. Yencho
    • 2
    Email author
  1. 1.Vegetable and Forage Crop Research UnitUSDA, ARSProsserUSA
  2. 2.Department of Horticultural ScienceNorth Carolina State UniversityRaleighUSA
  3. 3.Genetic Improvement of Fruits and Vegetables LaboratoryUSDA, ARSBeltsvilleUSA
  4. 4.Vernon G. James Research and Extension CenterNorth Carolina State UniversityPlymouthUSA

Personalised recommendations