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Assessment of genetic diversity of accessions in Brassicaceae genetic resources by frequency distribution analysis of S haplotypes

Abstract

Plant genetic resources are important sources of genetic variation for improving crop varieties as breeding materials. Conservation of such resources of allogamous species requires maintenance of the genetic diversity within each accession to avoid inbreeding depression and loss of rare alleles. For assessment of genetic diversity in the self-incompatibility locus (S locus), which is critically involved in the chance of mating, we developed a dot-blot genotyping method for self-incompatibility (S) haplotypes and applied it to indigenous, miscellaneous landraces of Brassica rapa, provided by the IPK Gene Bank (Gatersleben, Germany) and the Tohoku University Brassica Seed Bank (Sendai, Japan), in which landraces are maintained using different population sizes. This method effectively determined S genotypes of more than 500 individuals from the focal landraces. Although our results suggest that these landraces might possess sufficient numbers of S haplotypes, the strong reduction of frequencies of recessive S haplotypes occurred, probably owing to genetic drift. Based on these results, we herein discuss an appropriate way to conserve genetic diversity of allogamous plant resources in a gene bank.

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Acknowledgments

We are grateful to the IPK, Gatersleben, Germany and Kaneko Seed Co. LTD., Maebashi, Japan for providing plant materials, Yutaka Sato, Ryo Fujimoto, Kenta Shirasawa, Shunsuke Okamoto, and Tetsu Sugimura for helpful advice, Sylvain Billiardand for allowing us to use her Mathematica notebook, and Ohmi Ohnishi, Taihachi Kawahara, and Yasuo Yasui for encouragement. This work was supported in part by a Grant-in-Aid for Scientific Research (A) (no. 19208001) from the Japan Society for the Promotion of Science (JSPS). S. T. is a recipient of a research fellowship from JSPS for Young Scientists.

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Correspondence to T. Nishio.

Additional information

Communicated by R. Visser.

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Takuno, S., Oikawa, E., Kitashiba, H. et al. Assessment of genetic diversity of accessions in Brassicaceae genetic resources by frequency distribution analysis of S haplotypes. Theor Appl Genet 120, 1129–1138 (2010). https://doi.org/10.1007/s00122-009-1240-x

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Keywords

  • Genetic Drift
  • Dominance Relationship
  • Neutral Locus
  • Brassicaceae Species
  • Allogamous Species