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QTL mapping for reaction to Phaeosphaeria leaf spot in a tropical maize population

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Abstract

Phaeosphaeria leaf spot (PLS) is an important disease in tropical and subtropical maize (Zea mays, L.) growing areas, but there is limited information on its inheritance. Thus, this research was conducted to study the inheritance of the PLS disease in tropical maize by using QTL mapping and to assess the feasibility of using marker-assisted selection aimed to develop genotypes resistance to this disease. Highly susceptible L14-04B and highly resistant L08-05F inbred lines were crossed to develop an F2 population. Two-hundred and fifty six F2 plants were genotyped with 143 microsatellite markers and their F2:3 progenies were evaluated at seven environments. Ten plants per plot were evaluated 30 days after silk emergence following a rating scale, and the plot means were used for analyses. The heritability coefficient on a progeny mean basis was high (91.37%), and six QTL were mapped, with one QTL on chromosomes 1, 3, 4, and 6, and two QTL on chromosome 8. The gene action of the QTL ranged from additive to partial dominance, and the average level of dominance was partial dominance; also a dominance × dominance epistatic effect was detected between the QTL mapped on chromosome 8. The phenotypic variance explained by each QTL ranged from 2.91 to 11.86%, and the joint QTL effects explained 41.62% of the phenotypic variance. The alleles conditioning resistance to PLS disease of all mapped QTL were in the resistant parental inbred L08-05F. Thus, these alleles could be transferred to other elite maize inbreds by marker-assisted backcross selection to develop hybrids resistant to PLS disease.

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Acknowledgments

This research was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP-99/12143-1 and 01/05702-6) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-2003/302653). C.L. Souza Jr. and A.P. Souza are recipients of a research fellowship from CNPq. The authors express their gratitude to A.S. Oliveira, A.O. Gil, J. Monteiro and C.R. Segatelli for their assistance with the field experiments. We also thank two anonymous reviewers for their valuable contributions to improve the paper.

Author information

Correspondence to Cláudio Lopes de Souza Jr..

Additional information

Communicated by B. Godshalk.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 301 kb) Genetic map of chromosomes 1 to 4. The SSR markers are on the right of the map, and the map distances, in centimorgans (cM), from the first marker, are on the left of the chromosomes. The flanking markers of the mapped QTL are indicated with black boxes with arrows, and the QTL were named as Ph (Phaeosphaeria) followed by a number to identify the chromosome number. The dark markers on the chromosomes are the QTL positions and the elliptical markers are the centromeres

Supplementary material 2 (DOC 103 kb) Genetic map of chromosomes 5 to 10. The SSR markers are on the right of the map, and the map distances, in centimorgans (cM), from the first marker, are on the left of the chromosomes. The flanking markers of the mapped QTL are indicated with black boxes with arrows, and the QTL were named as Ph (Phaeosphaeria) followed by a number to identify the chromosome number and by a word (for more than one QTL on the chromosome). The dark markers on the chromosomes are the QTL positions and the elliptical markers are the centromeres

Supplementary material 3 (DOC 26 kb) Values and significances of the mean squares from the joint analysis of variance for reaction of maize F2:3 progenies to phaeosphaeria leaf spot disease

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Moreira, J.U.V., Bento, D.A.V., de Souza, A.P. et al. QTL mapping for reaction to Phaeosphaeria leaf spot in a tropical maize population. Theor Appl Genet 119, 1361–1369 (2009). https://doi.org/10.1007/s00122-009-1140-0

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Keywords

  • Maize
  • Phenotypic Variance
  • Genetic Correlation
  • Dominance Effect
  • Epistatic Effect