Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

QTL mapping for adult-plant resistance to stripe rust in Italian common wheat cultivars Libellula and Strampelli

Abstract

Italian common wheat cultivars Libellula and Strampelli, grown for over three decades in Gansu province of China, have shown effective resistance to stripe rust. To elucidate the genetic basis of the resistance, F3 populations were developed from crosses between the two cultivars and susceptible Chinese wheat cultivar Huixianhong. The F3 lines were evaluated for disease severity in Beijing, Gansu and Sichuan from 2005 to 2008. Joint- and single-environment analyses by composite interval mapping identified five quantitative trait loci (QTLs) in Libellula for reduced stripe rust severity, designated QYr.caas-2DS, QYr.caas-4BL, QYr.caas-5BL.1, QYr.caas-5BL.2 and QYr.caas-7DS, and explained 8.1–12.4, 3.6–5.1, 3.4–8.6, 2.6 and 14.6–35.0%, respectively, of the phenotypic variance across four environments. Six interactions between different pairs of QTLs explained 3.2–7.1% of the phenotypic variance. The QTLs QYr.caas-4BL, QYr.caas-5BL.1 and QYr.caas-7DS were also detected in Strampelli, explaining 4.5, 2.9–5.5 and 17.1–39.1% of phenotypic variance, respectively, across five environments. Three interactions between different pairs of QTLs accounted for 6.1–35.0% of the phenotypic variance. The QTL QYr.caas-7DS flanked by markers csLV34 and Xgwm295 showed the largest effect for resistance to stripe rust. Sequence analyses confirmed that the lines with the QYr.caas-7DS allele for resistance carried the resistance allele of the Yr18/Lr34 gene. Our results indicated that the adult-plant resistance gene Yr18 and several minor genes confer effective durable resistance to stripe rust in Libellula and Strampelli.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2

References

  1. Bariana HS, Kailasapillai S, Brown GN, Sharp PJ (1998) Marker assisted identification of Sr2 in the National Cereal Rust Control Program in Australia. In: Slinkard AE (ed) Proceedings of 9th international wheat and genetic symposium, vol 3, University of Extension Press, University of Saskatchewan, Saskatoon, pp 38–91

  2. Bariana HS, Hayden MJ, Ahmed NU, Bell JA, Sharp PJ, McIntosh RA (2001) Mapping of durable adult plant and seedling resistances to stripe rust and stem rust diseases in wheat. Aust J Agric Res 52:1247–1255

  3. Bassam BJ, Caetano-Anollés G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80–83

  4. Börner A, Schumann E, Fürste A, Cöster H, Leithold B, Röder MS, Weber WE (2002) Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet 105:921–936

  5. Borojevic K, Borojevic K (2005) Historic role of the wheat variety Akakomugi in southern and central European wheat breeding programs. Breed Sci 55:253–256

  6. Boukhatem N, Baret PV, Mingeot D, Jacquemin JM (2002) Quantitative trait loci for resistance against yellow rust in two wheat-derived recombinant inbred line populations. Theor Appl Genet 104:111–118

  7. Carlborg Ö, Haley C (2004) Epistasis: too often neglected in complex trait studies? Nat Rev Genet 5:618–625

  8. Chen XM (2005) Epidemiology and control of stripe rust (Puccinia striiformis f. sp. tritici) on wheat. Can J Plant Pathol 27:314–337

  9. Chen XM, Line RF (1995) Gene action in wheat cultivars for durable, high-temperature, adult-plant resistance and interaction with race-specific, seedling resistance to Puccinia striiformis. Phytopathology 85:567–572

  10. Fu DL, Uauy C, Distelfeld A, Blechl A, Epstein L, Chen XM, Sela H, Fahima T, Dubcovsky J (2009) A kinase-start gene confers temperature-dependent resistance to wheat stripe rust. Science 323:1357–1360

  11. Guyomarc’h H, Sourdille P, Charmet G, Edwards KJ, Bernard M (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat. Theor Appl Genet 104:1164–1172

  12. Islam MR, Shepherd KW, Mayo GME (1989) Recombination among genes at the L group in flax conferring resistant to rust. Theor Appl Genet 77:540–546

  13. Kolmer JA, Singh RP, Garvin DF, Viccars L, William HM, Huerta-Espino J, Ogbonnaya FC, Raman H, Orford S, Bariana HS, Lagudah ES (2008) Analysis of the Lr34/Yr18 rust resistance region in wheat germplasm. Crop Sci 48:1841–1852

  14. Kosambi DD (1944) The estimation of map distance from recombination values. Annu Eugen 12:172–175

  15. Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1363

  16. Lagudah ES, McFadden H, Singh RP, Huerta-Espino J, Bariana HS, Spielmeyer W (2006) Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor Appl Genet 114:21–30

  17. Li GQ, Li ZF, Yang WY, Zhang Y, He ZH, Xu SC, Singh RP, Qu YY, Xia XC (2006) Molecular mapping of stripe rust resistance gene YrCH42 in Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and Yr26. Theor Appl Genet 112:1434–1440

  18. Li HH, Ye GY, Wang JK (2007a) A modified algorithm for the improvement of composite interval mapping. Genetics 175:361–374

  19. Li Q, Jing JX, Wang BT, Zhou XC, Du JY (2007b) Genetic analysis of resistance to stripe rust in durable resistance wheat variety Libellula. Acta Phytophylacica Sinica 34:432–433

  20. Li HH, Li Z, Wang JK (2008) Inclusive composite interval mapping (ICIM) for digenic epistasis of quantitative traits in biparental population. Theor Appl Genet 116:243–260

  21. Liang SS, Suenaga K, He ZH, Wang ZL, Liu HY, Wang DS, Singh RP, Sourdille P, Xia XC (2006) Quantitative trait loci mapping for adult-plant resistance to powdery mildew in bread wheat. Phytopathology 96:784–789

  22. Lin F, Chen XM (2007) Genetics and molecular mapping of genes for race-specific all-stage resistance and non-race-specific high-temperature adult-plant resistance to stripe rust in spring wheat cultivar Alpowa. Theor Appl Genet 114:1277–1287

  23. Lin F, Chen XM (2009) Quantitative trait loci for non-race-specific, high-temperature adult-plant resistance to stripe rust in wheat cultivar Express. Theor Appl Genet 118:631–642

  24. Line RF (2002) Stripe rust of wheat and barley in North America: a retrospective historical review. Annu Rev Phytopathol 40:75–118

  25. Liu SX, Griffey CA, Maroof MAS (2001) Identification of molecular markers associated with adult plant resistance to powdery mildew in common wheat cultivar Massey. Crop Sci 41:1268–1275

  26. Mallard S, Gaudet D, Aldeia, Abelard C, Besnard AL, Sourdille P, Dedryver F (2005) Genetic analysis of durable resistance to yellow rust in bread wheat. Theor Appl Genet 110:1401–1409

  27. Manly KF, Cudmore RH Jr, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Genome 12:930–932

  28. McIntosh RA, Yamazaki Y, Devos KM, Dubcovsky J, Rogers J, Appels R (2003) Catalogue of gene symbols for wheat. http://www.grs.nig.ac.jp/wheat/komugi/genes

  29. Navabi A, Singh RP, Tewari JP, Briggs KG (2004) Inheritance of high levels of adult-plant resistance to stripe rust in five spring wheat genotypes. Crop Sci 44:1156–1162

  30. Nelson JC, Singh RP, Autrique JE, Sorrells ME (1997) Mapping genes conferring and suspecting leaf rust resistance in wheat. Crop Sci 37:1928–1935

  31. Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can J Res Sect C 26:496–500

  32. Ramburan VP, Pretorius ZA, Louw JH, Boyd LA, Smith PH, Boshoff WHP, Prins R (2004) A genetic analysis of adult plant resistance to stripe rust in the wheat cultivar Kariega. Theor Appl Genet 108:1426–1433

  33. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

  34. Rosewarne GM, Singh RP, Huerta-Espino J, Rebetzke GJ (2008) Quantitative trait loci for slow-rusting resistance in wheat to leaf rust and stripe rust identified with multi-environment analysis. Theor Appl Genet 116:1027–1034

  35. Santra DK, Chen XM, Santra M, Campbell KG, Kidwell KK (2008) Identification and mapping QTL for high-temperature adult-plant resistance to stripe rust in winter wheat (Triticum aestivum L.) cultivar Stephens. Theor Appl Genet 117:793–802

  36. Sharp PJ, Kreis M, Shewry PR, Gale MD (1988) Location of β-amylase sequence in wheat and its relatives. Theor Appl Genet 75:286–290

  37. Singh RP, Rajaram S (1994) Genetics of adult plant resistance to stripe rust in ten spring bread wheats. Euphytica 72:1–7

  38. Singh RP, Huerta-Espino J, Rajaram S (2000a) Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathol Entomol Hungarica 35:133–139

  39. Singh RP, Nelson JC, Sorrells ME (2000b) Mapping Yr28 and other genes for resistance to stripe rust in wheat. Crop Sci 40:1148–1155

  40. Singh RP, Huerta-Espino J, William HM (2005) Genetics and breeding for durable resistance to leaf and stripe rusts in wheat. Turk J Agric For 29:121–127

  41. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum asetivum L.). Theor Appl Genet 109:1105–1114

  42. Spielmeyer W, Sharp PJ, Lagudah ES (2003) Identification and validation of markers linked to broad-spectrum stem rust resistance gene Sr2 in wheat (Triticum aestivum L.). Crop Sci 43:333–336

  43. Spielmeyer W, Singh RP, McFadden H, Wellings CR, Huerta-Espino J, Kong X, Appels R, Lagudah ES (2008) Fine scale genetic and physical mapping using interstitial deletion mutants of Lr34/Yr18: a disease resistance locus effective against multiple pathogens in wheat. Theor Appl Genet 116:481–490

  44. Stubbs RW (1985) Stripe rust. In: Roelfs AP, Bushnell WR (eds) The cereal rusts II. Academic Press, Orlando, FL, pp 61–101

  45. Suenaga K, Singh RP, Huerta-Espino J, William HM (2003) Microsatellite markers for genes Lr34/Yr18 and other quantitative trait loci for leaf rust and stripe rust resistance in bread wheat. Phytopathology 93:881–890

  46. Uauy C, Brevis JC, Chen XM, Khan I, Jackson L, Chicaiza O, Distelfeld A, Fahima T, Dubcovsky J (2005) High-temperature adult-plant (HTAP) stripe rust resistance gene Yr36 from Triticum turgidum ssp. dicoccoides is closely linked to the grain protein content locus Gpc-B1. Theor Appl Genet 112:97–105

  47. Wan AM, Zhao ZH, Chen XM, He ZH, Jin SL, Jia QZ, Yao G, Yang JX, Wang BT, Li GB, Bi YQ, Yuan ZY (2004) Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici. Plant Dis 88:896–904

  48. Wang S, Basten CJ, Zeng ZB (2005) Windows QTL Cartographer v2.5. Statistical genetics. North Carolina State University, Raleigh, NC

  49. William HM, Singh RP, Huerta-Espino J, Ortiz-Islas S, Hoisington D (2003) Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat. Phytopathology 93:153–159

  50. William HM, Singh RP, Huerta-Espino J, Palacios G, Suenaga K (2006) Characterization of genetic loci conferring adult plant resistance to leaf rust and stripe rust in spring wheat. Genome 49:977–990

  51. Yang WX, Yang FP, Liang D, He ZH, Shang XW, Xia XC (2008) Molecular characterization of slow-rusting genes Lr34/Yr18 in Chinese wheat cultivars. Acta Agronom Sinica 34:1109–1113

  52. Yin XG, Zhang YH, Yan QJ, Shang XW (2005) Resistant characteristics to stripe rust and genetic analysis of durable resistance on wheat cultivar Strampelli. J. Plant Genet Res 6:390–393

  53. Yin XG, Shang XW, Song JR, Zhang YH, Yan QJ (2006) Genetic mechanism of durable resistance to stripe rust of wheat cultivar Libellula. J Triticeae Crops 26:147–150

  54. Young ND (1996) QTL mapping and quantitative disease resistance in plants. Annu Rev Phytopathol 34:479–501

  55. Zhang ZJ, Yang GH, Li GH, Jin SL, Yang XB (2001) Transgressive segregation, heritability, and number of genes controlling durable resistance to stripe rust in one Chinese and two Italian wheat cultivars. Phytopathology 91:680–686

  56. Zheng DS (1993) Use of Italian wheat varieties in China. Genet Resour Crop Evol 40:137–142

  57. Zhou XC, Du JY, Yang JH (2003a) A 30 successive years’ observation on the performance of several wheat cultivars in resistance to stripe rust (Puccinia striiformis West.) in the southern region of Gansu province of China. Acta Phytopath Sinica 33:550–554

  58. Zhou WC, Kolb FL, Bai GH, Domier LL, Boze LK, Smith NJ (2003b) Validation of a major QTL for scab resistance with SSR markers and use of marker assisted selection in wheat. Plant Breed 122:40–46

Download references

Acknowledgments

The authors are very grateful to Prof. R. A. McIntosh, Plant Breeding Institute, University of Sydney for the critical review of this manuscript. This study was supported by the National Science Foundation of China (30671294 and 30810214).

Author information

Correspondence to Xianchun Xia or Zhonghu He.

Additional information

Y. Lu and C. Lan contributed equally to this work.

Communicated by J. Dubcovsky.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lu, Y., Lan, C., Liang, S. et al. QTL mapping for adult-plant resistance to stripe rust in Italian common wheat cultivars Libellula and Strampelli. Theor Appl Genet 119, 1349–1359 (2009). https://doi.org/10.1007/s00122-009-1139-6

Download citation

Keywords

  • Simple Sequence Repeat Marker
  • Stripe Rust
  • Fusarium Head Blight Resistance
  • Leaf Rust Resistance
  • Stripe Rust Resistance