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Inheritance of the number and thickness of cell layers in barley aleurone tissue (Hordeum vulgare L.): an approach using F2–F3 progeny

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Abstract

The aleurone tissue of cereal grains, nutritionally rich in minerals and vitamins, is an important target for the improvement of cereals. Inheritance of the thickness and the number of cell layers in barley aleurone was studied on the F2–F3 progeny of an Erhard Frederichen × Criolla Negra cross in which the parental lines have three or two aleurone layers, respectively. F3 grain was sampled from each F2 plant and 96.8% of the entire F3 grain population was classified as being either the 2- or 3-layer type. Using microsatellite, single nucleotide polymorphism (SNP) and morphological markers on 190 F2 plants, a linkage map was built. Three quantitative trait loci (QTLs) affecting aleurone traits were revealed on chromosome 5H (max. LOD = 5.83) and chromosome 7H (max. LOD = 4.45) by interval mapping, and on chromosome 2H by marker analysis with an unmapped marker. These QTLs were consistent with genetic sub-models involving either 2-cell type dominance for 7H and 2H, or putative partial dominance for 5H where 2-cell-layer dominance and additivity gave similar LODs. The number of aleurone cell layers and aleurone thickness were strongly correlated and QTL results for these traits were alike. An SNP marker of sal1, an orthologue of the maize multilayer aleurone gene was mapped to the 7HL chromosome arm. However, the 7H QTL did not co-locate with the barley sal1 SNP, suggesting that an additional gene is involved in determining aleurone traits. These new mapping data allow comparisons to be made with related studies.

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Acknowledgments

We are grateful to W. Podyma and I. Loskutov for providing seeds of barley germplasm, A. Graner and M. Röder for SSR primers from IPK, L. Zhang and P. Sourdille for CFE markers, C. Dupré (†) and P. A. Meunier for cryogenic microtome sectioning and microscopy, G. Gay and N. Guilhot for microscopy and QTL pictures, and FX. Oury and F. Vear for critical reading of the manuscript.

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Correspondence to Louis Jestin.

Additional information

Communicated by A. Graner.

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Jestin, L., Ravel, C., Auroy, S. et al. Inheritance of the number and thickness of cell layers in barley aleurone tissue (Hordeum vulgare L.): an approach using F2–F3 progeny. Theor Appl Genet 116, 991–1002 (2008). https://doi.org/10.1007/s00122-008-0730-6

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Keywords

  • Aleurone Layer
  • Unlinked Marker
  • Barley Aleurone Layer
  • Aleurone Tissue
  • Large Sampling Error