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Genetic transformation of plastids of different Solanaceae species using tobacco cells as organelle hosts


The plastid genome of angiosperms represents an attractive target for genetic manipulations. However plastid transformation of higher plants, especially of agriculturally valuable crops is an extremely difficult problem. Transformation protocols developed for tobacco 15 years ago failed to produce similar results with more than a handful of other species so far. We have analyzed plastid transformability of remote cytoplasmic hybrids (cybrids) that combine nuclei of tobacco, an easily transformable species, and plastids of some other, recalcitrant Solanaceae species. Here, we demonstrate that the plastids of five species of Solanaceae family, representing two subfamilies and three tribes, can be easily transformed if the plastids of these species are transferred into a cell of a transformable species (tobacco). The results can be considered to be an alternative approach to the development of plastid transformation technologies for recalcitrant species using a transformable intermediary (“clipboard”) host.

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Gene of aminoglycoside-3′-adenylyntransferase


Internal transcribed spacer

ndhD :

Gene of NADH-dehydrogenase ND4 subunit


Polyethylene glycol


Gene of D1-protein (PS II)


Rubisco large subunit gene


Ribosome binding site


Gene of 50S ribosome protein CL22


Gene of 50S ribosome protein CL32


Gene of 30S ribosome protein CS19


Gene of Leu-tRNA


Gene of β-glucuronidase


Untranslated region


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The authors are grateful to Prof. Dr. H.-U. Koop, Munich University, Munich, Germany, and Dr. P. Medgyesy, Biological Research Centre, Szeged, Hungary, for providing research materials used in this study. We also thank Bob Erwin (LSBC, CA) for helpful suggestion and critical reading of the manuscript.

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Correspondence to Nikolay Kuchuk.

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Communicated by R. Hagemann

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Kuchuk, N., Sytnyk, K., Vasylenko, M. et al. Genetic transformation of plastids of different Solanaceae species using tobacco cells as organelle hosts. Theor Appl Genet 113, 519–527 (2006).

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  • Plastid Genome
  • Plastid Transformation
  • Solanaceae Family
  • aadA Gene
  • Solanaceae Species