Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

QTLs for drought escape and tolerance identified in a set of random introgression lines of rice

  • 653 Accesses

  • 84 Citations

Abstract

A large set of 254 introgression lines in an elite indica genetic background were evaluated for grain yield (GY) and related traits under the irrigated (control) and drought (stress) conditions in two consecutive years for genetic dissection of adaptive strategies of rice to water stress. A total of 36 quantitative trait loci (QTLs) affecting heading date (HD), plant height (PH), GY and yield components were identified and most QTLs showed pronounced differential expression either qualitatively or quantitatively in response to drought. These QTLs could be grouped into three major types based on their behaviors under control and stress conditions. Type I included 12 QTLs that expressed under both the stress and non-stress conditions. Type II comprised 17 QTLs that expressed under irrigation but not under stress. Type III included seven QTLs that were apparently induced by stress. The observation that the Lemont (japonica) alleles at all HD QTLs except QHd5 resulted in early heading under stress appeared to be responsible for the putative adaptation of Lemont to drought by escaping, whereas the Teqing (indica) alleles at most PH/GY QTLs were consistently associated with increased yield potential and trait stability and thus contributed to DT. Our result that most DT QTLs were non-allelic with QTLs for drought escaping suggests that the two adaptive strategies in the parental lines are under possible negative regulation of two largely non-overlapping genetic systems.

This is a preview of subscription content, log in to check access.

Fig. 1

References

  1. Araus JL, Slafer GA, Reynolds MP, Royo C (2002) Plant breeding and drought in C3 cereals: what should we breed for? Ann Bot 89:925–940

  2. Babu RC, Shanmugasundaram P, Chezhian P, Nguyen BD, Jayaprakash P, Ganesh SK, Chamarerk V, Palchamy A, Sadasivam S, Sarkarung S, Wade L, Nguyen HT (2002) Molecular dissection of drought tolerance in rice: association between physiomorphological traits and field performance. In: Saxena NP, O’Toole JC (eds) Field screening for drought tolerance in crop plants with emphasis on rice. Proceedings of an international workshop on field screening for drought tolerance in rice, December 11–14, 2000. ICRISAT, Patancheru, pp 82–91

  3. Bernacchi D, Beck-Bunn T, Emmatty D, Eshed Y, Inai s, Lopez J, Petiard V, Sayama H, Uhlig J, Zamir D, Tanksley S (1998) Advanced backcross QTL analysis of tomato. II. Evaluation of near-isogenic lines carrying single-donor introgressions for desirable wild QTL-alleles derived from Lycopersicon hirsutum and pimpinellifolium. Theor Appl Genet 97:170–180

  4. Blum A (2002) Drought tolerance — is it a complex trait? In: Saxena NP, O’Toole JC (eds) Field screening for drought tolerance in crop plants with emphasis on rice: proceedings of an international workshop on field screening for drought tolerance in rice, 11–14 Dec 2000. ICRISAT, Patancheru, and the Rockefeller Foundation, New York, pp 17–22

  5. Champoux MC, Wang G, Sarkarung S, Mackill DJ, O’Toole JC, Huang N, McCouch SR (1995) Locating genes associated with root morphology and drought avoidance in rice via linkage to molecular markers. Theor Appl Genet 90:969–981

  6. Graybill FA (1976) Theory and application of the linear model. Wadsworth and Brooks/Cole, Advanced Books and Software, Pacific Grove

  7. Huang XQ, Coster H, Ganal MW, Roder MS (2003) Advanced backcross QTL analysis for the identification of quantitative trait loci alleles from wild relatives of wheat (Triticum aestivum L.). Theor Appl Genet 106(8):1379–1389

  8. Lafitte HR, Courtois B (2000) Genetic variation in performance under reproductive stage water deficit in a doubled-haploid rice population in upland fields. In: Ribaut JM, Poland D (eds) Molecular approaches for the genetic improvement of cereals for stable production in water-limited environments. A strategic planning workshop held on 21–25 June 1999. CIMMYT, El Batan, pp 97–102

  9. Lafitte HR, Price AH, Courtois B (2004) Yield response to water deficit in an upland rice mapping population: associations among traits and genetic markers. Theor Appl Genet 109:1237–1246

  10. Lafitte HR, Vijayakumar CHM, Gao YM, Shi Y, Xu JL, Fu BY, Yu SB, Ali AJ, Domingo J, Maghirang R, Torres R, Mackill D, Li ZK (2005) Improvement of rice drought tolerance through backcross breeding: evaluation of donors and results from drought nurseries. Field Crop Research (in press)

  11. Lanceras JC, Pantuwan G, Jongdee B, Toojinda T (2004) Quantitative trait loci associated with drought tolerance at reproductive stage in rice. Plant Physiol 135:384–399

  12. Li Z, Shen LS, Courtois B, Lafitte R (2000) Development of near-isogenic introgression line (NIIL) sets for QTLs associated with drought tolerance in rice. In: Ribaut JM, Poland D (eds) Molecular approaches for the genetic improvement of cereals for stable production in water-limited environments. A strategic planning workshop held on 21–25 June 1999. CIMMYT, El Batan, pp 103–107

  13. Li ZK, Luo LJ, Mei HW, Shu QY, Wang DL, Tabien R, Zhong DB, Ying CS, Stansel JW, Khush GS, Paterson AH (2001) Overdominant epistatic loci are the primary genetic basis of inbreeding depression and heterosis in rice: I. Biomass and grain yield. Genetics 158:1737–1753

  14. Li ZK, Pinson SRM, Park WD, Paterson AH, Stansel JW (1997) Epistasis for three grain yield components in rice (Oryxa sativa L.). Genetics 145:453–465

  15. Li ZK, Yu SB, Lafitte HR, Huang N, Courtois B, Hittalmani S, Vijayakumar CH, Liu GF, Wang GC, Shashidhar HE, Zhuang JY, Zheng KL, Singh VP, Sidhu S, Srivantaneeyakul S, Khush GS (2003) QTL × environment interactions in rice. I. heading date and plant height. Theor Appl Genet 108:141–153

  16. Price AH, Tomos AD (1997) Genetics dissection of root growth in rice (Oryza sativa L.) II: Mapping quantitative trait loci using molecular markers. Thero Appl Genet 95:143–152

  17. Ray JD, Yu L, McCouch SR, Champoux MC, Wang G, Nguyen HT (1996) Mapping quantitative trait loci associated with root penetration ability in rice. Theor Appl Genet 92:627–933

  18. SAS Institute (1996) SAS/STAT User’s Guide. SAS Institute, Cary

  19. Shen L, Courtois B, McNally K, Robin S, Li ZK (2001) Evaluation of near-isogenic lines of rice introgressed with QTLs for root traits through marker-aided selection. Theor Appl Genet 103:70–83

  20. Tanksley SD, Nelson JC (1996) Advanced backcross QTL analysis: a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Thero Appl Genet 92:191–203

  21. Thomson MJ, Tai TH, McClung AM, Lai XH, Hinga ME, Lobos KB, Xu Y, Martinez CP, McCouch SR (2003) Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson. Theor Appl Genet 107:479–493

  22. Wang DL, Zhu J, Li ZK, Paterson AH (1999) Mapping QTLs with epistatic effects and QTL × environment interactions by mixed linear model approaches. Theor Appl Genet 99:1255–1264

  23. Widawsky DA, O’Toole JC (1990) Prioritizing rice biotechnology research agenda for Eastern India. The Rockefeller Foundation, New York

  24. Yoshida S, de los Reyes E (1976) Leaf cuticular resistance of rice varieties. Soil Sci Plant Nutr 22:95–98

  25. Zhang J, Zheng HG, Aarti A, Pantuwan G, Nguyen TT, Tripathy JN, Sarial AK, Robin S, Babu RC, Nguyen BD, Sarkarung S, Blum A, Nguyen HT (2001) Locating genomic regions associated with components of drought resistance in rice: comparative mapping within and across species. Theor Appl Genet 103:19–29

Download references

Acknowledgements

We are grateful to Dr. McCouch of Cornell University for providing us with the sequence information of the SSR markers. This study was supported by grants from the Rockefeller Foundation, BMZ/GTZ of the Germany Government to Z. K. Li/H. R. Lafitte, and by the 973 and 863 Project from the Chinese Ministry of Science and Technology and the ‘948’ Program from the Chinese Ministry of Agriculture.

Author information

Correspondence to Z. K. Li.

Additional information

Communicated by T. Sasaki

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Xu, J.L., Lafitte, H.R., Gao, Y.M. et al. QTLs for drought escape and tolerance identified in a set of random introgression lines of rice. Theor Appl Genet 111, 1642–1650 (2005). https://doi.org/10.1007/s00122-005-0099-8

Download citation

Keywords

  • Plant Height
  • Drought Tolerance
  • Grain Yield
  • Head Date
  • Irrigate Condition