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Introgression of wheat DNA markers from A, B and D genomes in early generation progeny of Aegilops cylindrica Host  ×  Triticum aestivum L. hybrids

Abstract

Introgression from allohexaploid wheat (Triticum aestivum L., AABBDD) to allotetraploid jointed goatgrass (Aegilops cylindrica Host, CCDD) can take place in areas where the two species grow in sympatry and hybridize. Wheat and Ae. cylindrica share the D genome, issued from the common diploid ancestor Aegilops tauschii Coss. It has been proposed that the A and B genome of bread wheat are secure places to insert transgenes to avoid their introgression into Ae. cylindrica because during meiosis in pentaploid hybrids, A and B genome chromosomes form univalents and tend to be eliminated whereas recombination takes place only in D genome chromosomes. Wheat random amplified polymorphic DNA (RAPD) fragments, detected in intergeneric hybrids and introgressed to the first backcross generation with Ae. cylindrica as the recurrent parent and having a euploid Ae. cylindrica chromosome number or one supernumerary chromosome, were assigned to wheat chromosomes using Chinese Spring nulli-tetrasomic wheat lines. Introgressed fragments were not limited to the D genome of wheat, but specific fragments of A and B genomes were also present in the BC1. Their presence indicates that DNA from any of the wheat genomes can introgress into Ae. cylindrica. Successfully located RAPD fragments were then converted into highly specific and easy-to-use sequence characterised amplified regions (SCARs) through sequencing and primer design. Subsequently these markers were used to characterise introgression of wheat DNA into a BC1S1 family. Implications for risk assessment of genetically modified wheat are discussed.

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Acknowledgments

We are grateful to Prof. Beat Keller and Dr. Nabila Yahiaoui of the Institute of Plant Biology of the University of Zürich, Switzerland, for providing the nulli-tetrasomic wheat lines; to Dr. Eva Stoger of the Institute for Biology of the RWTH Aachen, Germany, for providing seeds of the wheat variety Bobwhite; to Dr. Yong-Ming Yuan and Dr. Philippe Chassot from our laboratory for advice during sequencing, to Anouk Béguin and Sarah Mamie for technical assistance, and to two anonymous reviewers for their helpful comments. This project was funded by the National Centre of Competence in Research (NCCR) Plant Survival, a research programme of the Swiss National Science Foundation.

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Correspondence to N. Schoenenberger.

Additional information

Communicated by B. Friebe

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Schoenenberger, N., Felber, F., Savova-Bianchi, D. et al. Introgression of wheat DNA markers from A, B and D genomes in early generation progeny of Aegilops cylindrica Host  ×  Triticum aestivum L. hybrids. Theor Appl Genet 111, 1338–1346 (2005). https://doi.org/10.1007/s00122-005-0063-7

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Keywords

  • Wheat Chromosome
  • Genome Chromosome
  • Allohexaploid Wheat
  • BC1S1 Family
  • Open Pollination Condition