Nuclear matrix attachment regions (MARs) are thought to influence the expression of the flanking genes. TM2, a new DNA fragment isolated from tobacco, can bind with the rice nuclear matrix in vitro. In this study, we investigated the effect of TM2 on transgene expression under the control of three different promoters in stably transformed rice calli and plants. The presence of TM2 flanking the transgene increased the expression of constructs based on the constitutive CaMV 35S and maize ubiquitin gene promoters in both resistant calli and transformed plants. The GUS expression directed by the photosynthetic-tissue-specific PNZIP promoter was also increased in photosynthetic tissues of transformants. However, TM2 did not change the gene expression pattern controlled by the PNZIP promoter. The effect of TM2 in transgenic plants was stronger than that in transgenic calli based on all three promoters. Our results indicate that TM2, as a novel strong MAR, can be used to increase the transgene expression levels in the whole plant or in particular tissues of monocotyledons.
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We thank Steven Spiker, professor of North Carolina State University, for helpful discussions. This work was supported by the National Special Program for Research and Industrialization of Transgenic Plants (grant no. J99-A-038), the National Science Foundation (grant no. 30270145) and the “863” project (grant no. 2002AA224101) in China.
Communicated by L. Willmitzer
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Xue, H., Yang, Y., Wu, C. et al. TM2, a novel strong matrix attachment region isolated from tobacco, increases transgene expression in transgenic rice calli and plants. Theor Appl Genet 110, 620–627 (2005). https://doi.org/10.1007/s00122-004-1880-9
- Transgenic Rice
- Nuclear Matrix
- Transgenic Rice Plant
- Matrix Attachment Region
- Soybean Heat Shock