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Mapping of quantitative trait loci associated with ultraviolet-B resistance in rice (Oryza sativa L.)

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Abstract

The detection of quantitative trait loci (QTLs) associated with UV-B resistance in rice should allow their practical application in breeding for such a complex trait, and may lead to the identification of gene characteristics and functions. Considerable variation in UV-B resistance exists within cultivated rice (Oryza sativa L.), but its detailed genetic control mechanism has not been well elucidated. We detected putative QTLs associated with the resistance to enhanced UV-B radiation in rice, using 98 BC1F5 (backcross inbred lines; BILs) derived from a cross between Nipponbare (a resistant japonica rice variety) and Kasalath (a sensitive indica rice variety). We used 245 RFLP markers to construct a framework linkage map. BILs and both parents were grown under visible light with or without supplemental UV-B radiation in a growth chamber. In order to evaluate UV-B resistance, we used the relative fresh weight of aerial parts (RFW) and the relative chlorophyll content of leaf blades (RCC). The BIL population exhibited a wide range of variation in RFW and RCC. Using composite interval mapping with a LOD threshold of 2.9, three putative QTLs associated with both RFW and RCC were detected on chromosomes 1, 3 and 10. Nipponbare alleles at the QTLs on chromosome 1 and 10 increased the RFW and RCC, while the Kasalath allele at the QTL on chromosome 3 increased both traits. Furthermore, the existence of both QTLs on chromosomes 1 and 10 for UV-B resistance was confirmed using chromosome segment substitution lines. Plants with Kasalath alleles at the QTL on chromosome 10 were more sensitive to UV-B radiation than plants with them on chromosome 1. These results also provide the information not only for the improvement of UV-B resistance in rice though marker-associated selection, but also for the identification of UV-B resistance mechanisms by using near-isogenic lines.

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Acknowledgements

This work was supported by Grants-in-Aid (No. 10556075, 12480154) for Scientific Research from the Ministry of Education, Culture and Science, Japan (Y.F.T.K. and T.S.), and by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Rice Genome Project MP-1121) (T.U. and M.Y.). The authors thank Dr. Takeshi Hayashi (National Institute of Agrobiological Sciences), Dr. Jun Hidema (Graduate School of Life Science, Tohoku University) and Dr. Tadahiko Mae (Graduate School of Agricultural Science, Tohoku University) for their useful suggestions. We are grateful to Mr. Syoichi Musashi for his expert technical assistance.

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Correspondence to T. Sato.

Additional information

Communicated by D.J. Mackill

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Sato, T., Ueda, T., Fukuta, Y. et al. Mapping of quantitative trait loci associated with ultraviolet-B resistance in rice (Oryza sativa L.). Theor Appl Genet 107, 1003–1008 (2003). https://doi.org/10.1007/s00122-003-1353-6

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Keywords

  • Oryza sativa L.
  • Backcross inbred lines
  • Chromosome segment substitution lines
  • QTL analysis
  • UV-B resistance