The detection of quantitative trait loci (QTLs) associated with UV-B resistance in rice should allow their practical application in breeding for such a complex trait, and may lead to the identification of gene characteristics and functions. Considerable variation in UV-B resistance exists within cultivated rice (Oryza sativa L.), but its detailed genetic control mechanism has not been well elucidated. We detected putative QTLs associated with the resistance to enhanced UV-B radiation in rice, using 98 BC1F5 (backcross inbred lines; BILs) derived from a cross between Nipponbare (a resistant japonica rice variety) and Kasalath (a sensitive indica rice variety). We used 245 RFLP markers to construct a framework linkage map. BILs and both parents were grown under visible light with or without supplemental UV-B radiation in a growth chamber. In order to evaluate UV-B resistance, we used the relative fresh weight of aerial parts (RFW) and the relative chlorophyll content of leaf blades (RCC). The BIL population exhibited a wide range of variation in RFW and RCC. Using composite interval mapping with a LOD threshold of 2.9, three putative QTLs associated with both RFW and RCC were detected on chromosomes 1, 3 and 10. Nipponbare alleles at the QTLs on chromosome 1 and 10 increased the RFW and RCC, while the Kasalath allele at the QTL on chromosome 3 increased both traits. Furthermore, the existence of both QTLs on chromosomes 1 and 10 for UV-B resistance was confirmed using chromosome segment substitution lines. Plants with Kasalath alleles at the QTL on chromosome 10 were more sensitive to UV-B radiation than plants with them on chromosome 1. These results also provide the information not only for the improvement of UV-B resistance in rice though marker-associated selection, but also for the identification of UV-B resistance mechanisms by using near-isogenic lines.
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Allen DJ, Nogues S, Baker NR (1998) Ozone depletion and increased UV-B radiation: is there a real threat to photosynthesis? J Exp Bot 49:1775–1788
Barnes PW, Maggard S, Holman SR, Vergara BS (1993) Intraspecific variation in sensitivity to UV-B radiation in rice. Crop Sci 33:1041–1046
Basten CJ, Weir BS, Zeng Z-B (2002) QTL Cartographer, version 1.16. A reference manual and tutorial for QTL mapping. Department of Statistics, North Carolina State University, Raleigh, North Carolina, USA
Blumthaler M, Ambach W (1990) Indication of increasing solar ultraviolet-B radiation flux in alpine regions. Science 248:206–208
Caldwell MM (1971) Solar UV irradiation and the growth and development of higher plants. In: Glese AC Photophysiology Vol. 6, Academic Press, New York, USA, pp 131–177
Caldwell MM, Robberecht R, Flint SD (1983) Internal filters: prospects for UV-acclimation in higher plants. Physiol Plant 58:445–450
Dai Q, Coronel VP, Vergara BS, Barnes PW, Quintos AT (1992) Ultraviolet-B radiation effects on growth and physiology of four rice cultivars. Crop Sci 32:1269–1274
Dai Q, Yan B, Huang S, Liu X, Peng S, Miranda MLL, Chavez AQ, Vergara BS, Olszyk DM (1997) Response of oxidative stress defense systems in rice (Oryza sativa) leaves with supplemental UV-B radiation. Physiol Plant 101:301–308
Elastner EF (1982) Oxygen activation and oxygen toxicity. Annu Rev Plant Physiol 35:15–44
Hidema J, Kumagai T (1998) UV-B-induced cyclobutyl pyrimidine dimer and photorepair with progress of growth and leaf age in rice. Photochem Photobiol 43:121–127
Hidema J, Kang H-S, Kumagai T (1996) Differences in the sensitivity to UVB radiation of two cultivars of rice (Oryza sativa L.). Plant Cell Physiol 37:742–747
Hidema J, Kumagai T, Sutherland JC, Sutherland BM (1997) Ultraviolet B-sensitive rice cultivar deficient in cyclobutyl pryimidine dimer repair. Plant Physiol 113:39–44
Hidema J, Kumagai T, Sutherland BM (2000) UV radiation-sensitive Norin 1 rice contains defective cyclobutane pyrimidine dimer photolyase. Plant Cell 12:1569–1578
Hidema J, Song I-K, Sato T, Kumagai T (2001) Relationship between ultraviolet-B sensitivity and cyclobutane pyrimidine dimer photorepair in rice. J Radiat Res 42:295–303
Hollósy F (2002) Effects of ultraviolet radiation on plant cells. Micron 33:179–197
Jansen RC (1996) Complex plant traits: time for polygenetic analysis. Trend Plant Sci 1:89–88
Kumagai T, Sato T (1992) Inhibitory effects of increase in near-UV radiation on the growth of Japanese rice cultivars (Oryza sativa L.) in a phytotron and recovery by exposure to visible radiation. Jpn J Breed 42:545–552
Kumagai T, Hidema J, Kang H-S, Sato T (2001) Effects of supplemental UV-B radiation on the growth and yield of two cultivars of Japanese lowland rice (Oryza sativa L.) under the field in a cool rice-growing region of Japan. Agr Ecosystems Environ 83:201–208
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Lin HX, Yamamoto T, Sasaki T, Yano M (2000) Characterization and detection of epistatic interactions of three QTLs, Hd1, Hd2 and Hd3, controlling heading date in rice using nearly isogenic lines. Theor Appl Genet 101:1021–1028
Lin SY, Sasaki T, Yano M (1998) Mapping quantitative trait loci controlling seed dormancy and heading date in rice, Qryza sativa L. using backcross inbred lines. Theor Appl Genet 96:997–1003
Ma JF, Shen RF, Zhao ZQ, Wissuwa M, Takeuchi Y, Ebitani T, Yano M (2002) Response of rice to Al stress and identification of quantitative trait loci for Al tolerance. Plant Cell Physiol 43:652–659
Mackerness SAH (2000) Plant responses to ultraviolet-B (UV-B: 280–320 nm) stress: what are the key regulators? Plant Growth Reg 32:27–39
McCouch SR, Doerge RW (1995) QTL mapping in rice. Trends Genet 11:482–487
Miura K, Lin SY, Yano M, Nagamine T (2001) Mapping quantitative trait loci controlling low temperature germinability in rice (Oryza sativa L.). Breed Sci 51:293–299
Miura K, Lin SY, Yano M, Nagamine T (2002) Mapping quantitative trait loci controlling seed longevity in rice (Oryza sativa L.) Theor Appl Genet 104:981–986
Obara M, Kajiura M, Fukuta Y, Yano M, Hayashi M, Yamaya T, Sato T (2001) Mapping of QTLs associated with cytosolic glutamine synthetase and NADH-glutamate synthase in rice (Oryza sativa L.). J Exp Bot 52:1209–1217
Sato T, Kumagai T (1993) Cultivar differences in resistance to the inhibitory effects of near-UV radiation among Asian ecotype and Japanese lowland and upland cultivars of rice (Oryza sativa L.). Jap J Breed 43:61–68
Sato T, Kumagai T (1997) Role of UV-absorbing compounds in genetic differences in the resistance to UV-B radiation in rice plants. Breed Sci 47:21–26
Sato T, Kang HS, Kumagai T (1994) Genetic study of resistance to inhibitory effects of UV radiation in rice (Oryza sativa). Physiol Plant 91:234–238
Searles PS, Flint SD, Caldwell MM (2001) A meta analysis of plant field studies simulating stratospheric ozone depletion. Oecologia 127:1–10
Tanksley SD (1993) Mapping polygenes. Annu Rev Genet 27:205–233
Teramura AH (1983) Effects of ultraviolet-B radiation on the growth and yield of crop plants. Physiol Plant 58:415–427
Teramura AH, Ziska LH, Sztein AE (1991) Changes in growth and photosynthetic capacity of rice with increased UV-B radiation. Physiol Plant 83:373–380
Tevini M, Teramura AH (1989) UV-B effects on terrestrial plants. Photochem Photobiol 50:479–487
Wintermans JFGM, Mots A De (1965) Spectrophotometric characteristics of chlorophylls a and b and their pheophytins in ethanol. Biochem Biophys Acta 109:448–453
Yano M (2001) Genetic and molecular dissection of naturally occurring variations. Curr Opin Plant Biol 4:130–135
Yano M, Sasaki T (1997) Genetic and molecular dissection of quantitative traits in rice. Plant Mol Biol 35:145–153
Yoshida S (1981) Fundamentals of rice crop science. International Rice Research Institute, Manila
This work was supported by Grants-in-Aid (No. 10556075, 12480154) for Scientific Research from the Ministry of Education, Culture and Science, Japan (Y.F.T.K. and T.S.), and by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Rice Genome Project MP-1121) (T.U. and M.Y.). The authors thank Dr. Takeshi Hayashi (National Institute of Agrobiological Sciences), Dr. Jun Hidema (Graduate School of Life Science, Tohoku University) and Dr. Tadahiko Mae (Graduate School of Agricultural Science, Tohoku University) for their useful suggestions. We are grateful to Mr. Syoichi Musashi for his expert technical assistance.
Communicated by D.J. Mackill
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Sato, T., Ueda, T., Fukuta, Y. et al. Mapping of quantitative trait loci associated with ultraviolet-B resistance in rice (Oryza sativa L.). Theor Appl Genet 107, 1003–1008 (2003). https://doi.org/10.1007/s00122-003-1353-6
- Oryza sativa L.
- Backcross inbred lines
- Chromosome segment substitution lines
- QTL analysis
- UV-B resistance