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The Science of Nature

, 105:59 | Cite as

Microbiota disruption leads to reduced cold tolerance in Drosophila flies

  • Youn Henry
  • Hervé Colinet
Short Communication

Abstract

It is now acknowledged that bacteria from gut microbiota deeply interact with their host by altering many physiological traits. Such interplay is likely to consequently affect stress tolerance. Here, we compared cold and heat tolerance of Drosophila melanogaster flies with undisrupted (control (Co)) versus disrupted gut microbiota (dechorionated eggs (De)). The disrupting treatment strongly reduced bacterial load in flies’ guts, though 16S sequencing analysis did not evidence strong diversity changes in the remaining bacterial community. Both chill coma recovery and acute cold survival were repeatedly lower in De than in Co flies under our experimental conditions. However, heat tolerance was not consistently affected by gut disruption. Our results suggest that microbiota-related effects on the host can alter ecologically relevant traits such as thermal tolerance.

Keywords

Gut microbiota Fruit flies Thermal stress Chill coma 

Notes

Acknowledgments

Authors thank the GeT-PLaGe plateform for Miseq sequencing.

Compliance with ethical standards

Competing interests

The authors declare that they have no conflict of interest.

Supplementary material

114_2018_1584_MOESM1_ESM.docx (22 kb)
ESM 1 (DOCX 21 kb)
114_2018_1584_MOESM2_ESM.png (138 kb)
Supplementary Figure 1 (PNG 138 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.UMR CNRS 6553 EcobioUniversité de Rennes1RennesFrance

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