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The monocyte-dependent immune response to bacteria is suppressed in smoking-induced COPD

  • Jürgen KnoblochEmail author
  • Susanne Panek
  • Sarah Derya Yanik
  • Kaschin Jamal Jameel
  • Zeynep Bendella
  • David Jungck
  • Paul Bürger
  • Eike Bülthoff
  • Birte Struck
  • Nikolaos Giannakis
  • Jan Rupp
  • Juliane Kronsbein
  • Marcus Peters
  • Andrea Koch
Original Article

Abstract

COPD patients have an increased susceptibility to bacterial airway infections that can induce exacerbations. In response to infections, circulating monocytes become recruited to the infected tissue and secrete cytokines. We hypothesized that this cytokine response is reduced in COPD. Cultured peripheral blood monocytes of never smokers (NS) and smokers without (S) and with COPD (3 study populations, n = 36–37) were stimulated with extracts of Haemophilus influenzae, Staphylococcus aureus, or Streptococcus pneumoniae or with four different pathogen-associated molecular patterns (PAMPs). Four cytokines and 9 PAMP-related signaling molecules were measured and compared between the groups. Granulocyte-macrophage-colony-stimulating-factor responses to all stimulants were reduced in S and COPD compared to NS. Tumor-necrosis-factor-α responses to all bacterial extracts, peptidoglycan, and lipopolysaccharide were reduced in S and/or COPD. Interleukin-10 responses to S. aureus and lipoteichoic acid were increased in COPD. Correlations to pack-years and lung function were found. The peptidoglycan-receptor NOD2 and the mRNA of the lipopolysaccharide-receptor TLR4 were reduced in S and COPD. Cytokine responses of monocytes to bacteria are suppressed by smoking and in COPD possibly due to NOD2 and TLR4 reduction and/or interleukin-10 increase. This might help to explain the increased susceptibility to bacterial infections. These systemic molecular pathologies might be targets for therapeutic strategies to prevent infection-induced exacerbations.

Key messages

  • COPD subjects have an increased susceptibility to bacterial infections.

  • This implies defects in the immune response to bacteria and is critical for disease progression.

  • The cytokine response of monocytes to bacteria is reduced in COPD.

  • This might be due to a reduced NOD2 and TLR4 and an increased IL-10 expression.

  • This can explain the increased susceptibility to infections and help to identify drug targets.

Keywords

COPD Immune defect Monocytes Bacterial infection 

Notes

Acknowledgements

We thank Sandra Körber, Carmen Meinig, and Katja Urban for excellent technical assistance.

Funding

This study was supported by the FoRUM Program of the Ruhr University Bochum, Germany (RUB, F716R-2011 to J.K. and M.P.).

Compliance with ethical standards

The study was approved by the Ethics Committees of the University of Cologne, Germany (02-004) and of the University of Bochum, Germany (4257-12, 4772-13) and all patients gave written consent.

Conflict of interest

Dr. Jungck reports payment for lectures from Novartis as well as travel support from Actelion, Boehringer Ingelheim, Teva, Sanofi, and Roche; Dr. Knobloch reports personal fees from AstraZeneca, personal fees from Teva, personal fees from Novartis, personal fees from Actelion Pharmaceuticals, and personal fees from Boehringer Ingelheim, outside the submitted work; Dr. Kronsbein reports personal fees from AstraZeneca, personal fees from Boehringer Ingelheim, and personal fees from Novartis, outside the submitted work; Dr. Peters reports personal fees from Biotest AG, personal fees from Protectimmun GmbH, grants from Dr. Georg E. und Marianne Kosing-Stiftung, grants from Georgius Agricola Stiftung Ruhr, and grants from Phenox GmbH, outside the submitted work; Dr. Bendella, Mr. Bülthoff, Dr. Bürger, Dr. Giannakis, Mrs. Jamal Jameel, Dr. Koch, Dr. Panek, Dr. Rupp, Mrs. Struck, and Dr. Yanik have nothing to disclose.

Supplementary material

109_2019_1778_MOESM1_ESM.docx (27 kb)
ESM 1 (DOCX 27.3 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Jürgen Knobloch
    • 1
    • 2
    Email author
  • Susanne Panek
    • 1
  • Sarah Derya Yanik
    • 1
  • Kaschin Jamal Jameel
    • 1
  • Zeynep Bendella
    • 2
    • 3
  • David Jungck
    • 1
    • 2
    • 4
  • Paul Bürger
    • 1
  • Eike Bülthoff
    • 1
  • Birte Struck
    • 1
  • Nikolaos Giannakis
    • 1
  • Jan Rupp
    • 5
  • Juliane Kronsbein
    • 1
  • Marcus Peters
    • 6
  • Andrea Koch
    • 7
    • 8
  1. 1.Medical Clinic III for Pneumology, Allergology, Sleep- and Respiratory MedicineBergmannsheil University HospitalBochumGermany
  2. 2.Department of Pneumology, Clinic III for Internal MedicineUniversity of CologneCologneGermany
  3. 3.Department of RadiologyUniversity of Bonn Medical CenterBonnGermany
  4. 4.Department of Internal Medicine II, Pneumology, Allergology and Respiratory MedicineBethel Teaching HospitalBerlinGermany
  5. 5.Department of Infectious Diseases and MicrobiologyUniversity of LübeckLübeckGermany
  6. 6.Department of Experimental PneumologyRuhr University BochumBochumGermany
  7. 7.Medical Clinic VLudwig-Maximilians-University LMUMunichGermany
  8. 8.German Center for Lung Research (DZL)MunichGermany

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