Functional characterization of two novel peptides and their analogs identified from the skin secretion of Indosylvirana aurantiaca, an endemic frog species of Western Ghats, India
Two new antimicrobial peptides belonging to brevinin 1 (B1) and brevinin 2 (B2) families were identified from the skin secretion of Indosylvirana aurantiaca, an endemic frog of Western Ghats, India, through shotgun cloning. Antibacterial, antibiofilm and cytotoxic effects of these peptides were evaluated and compared with their C-terminally amidated forms. Both the amidated peptides (B1-NH2 and B2-NH2) showed significantly enhanced broad-spectrum activities against Gram-positive and Gram-negative bacteria and the latter was found to have potent biofilm inhibition properties against Klebsiella pneumoniae over other peptides. Both forms of B2 were less cytotoxic against human red blood cells (hRBC) and peripheral blood mononuclear cell (PBMC). Hence, B2 and its amidated analog have good therapeutic value and these peptides could be considered as potential lead molecules for further development.
KeywordsAntibacterial Antibiofilm Brevinin Frog skin peptide Therapeutics
This work was financially supported by Kerala State Council for Science Technology and Environment under Verghese Kurien Young Scientist Fellowship Scheme (Order no. 717/2014/KSCSTE). SHG is thankful to The Director, Rajiv Gandhi Centre for Biotechnology, for providing facilities for conducting the work properly. The authors are also grateful to Kerala Forest Department for permitting sample collection.
Compliance with ethical standards
Conflict of interest
The authors declare that there are no conflicts of interests.
The protocols of the assays with human samples were approved by the human ethics committee of Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala.
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