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Component analysis and antiasthmatic effects of Huashanshen dripping pill

  • Shuli ManEmail author
  • Nina Cui
  • Xuanshuo Liu
  • Long Ma
  • Changxiao Liu
  • Wenyuan GaoEmail author
Original Research
  • 27 Downloads

Abstract

Huashanshen dripping pill (HSS), a commonly used traditional Chinese medicine, has been widely used in China due to its properties of antiasthma, expectorant, and antitussive. However, it was less in-depth understanding of the chemical composition, their absorption in plasma and potential biological mechanism. In this research, ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was developed to simultaneously identify the chemical constituents and absorption in rat plasma after oral administration of HSS. IgE-sensitized to ovalbumin was induced in BALB/c mice. ELISA and molecular docking analysis were carried out to identify their antiasthmatic mechanisms. As a result, 11 compounds were tentatively characterized by comparing the retention time, ion fragments, and the accurate mass measurement of [M + H]+ ions in the HSS. Two alkaloids including d-anisodamine and l-anisodamine were identified in the rat plasma after oral administration of HSS. The pills alleviated airway inflammation in lung tissues, and inhibited IgE, IL-4, and IL-5 in the serum. Furthermore, there were several amino acid residues involved in anisodamine-targeting receptors of hydrogen bonds and hydrophobic interactions via molecular docking indication. All in all, anisodamine would be the main component in the HSS whose antiasthmatic effects based on the inhibition of IgE, IL-4, and IL-5 production.

Keywords

Huashanshen dripping pill Traditional Chinese medicine UHPLC-QTOF-MS Molecular docking Anisodamine Asthma 

Abbreviations

DM

dexamethasone

ELISA

enzyme-linked immunosorbent assay

HSS

huashanshen dripping pill

IgE

immunoglobulin E

IL

interleukins

MS

mass spectrometry

OVA

ovalbumin

RT

the retention time

UHPLC-QTOF-MS

ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

Notes

Acknowledgements

This work was supported by grants 81673647, 81673535, and 81503086 from the National Natural Science Foundation of China.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, College of BiotechnologyTianjin University of Science and TechnologyTianjinChina
  2. 2.The State Key Laboratories of Pharmacodynamics and PharmacokineticsTianjinChina
  3. 3.Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and TechnologyTianjin UniversityTianjinChina

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