A new triterpene diglycoside from the roots of Bupleurum chinense DC. and its inhibitory effect on adipogensis in 3T3-L1 cells

  • Yujing Feng
  • Zhou-Wei Wu
  • Yanyan Luo
  • Liang Chen
  • Yufeng Cao
  • Lun Wang
  • Aftab Yaseen
  • Bin Chen
  • Ashfaq Ahmad Khan
  • Ming-Kui Wang
  • Guo-Lin Zhang
  • Xin-Feng WangEmail author
  • Fu LiEmail author
  • Xueqin Li
  • Weicheng HuEmail author
Original Research


A new triterpene diglycoside, tibesaikosaponin V (TKV), was isolated from the methanol extract of the roots of Bupleurum chinense DC. by normal and reversed phase column chromatography. The chemical structure of this new compound was elucidated based on extensive spectroscopic analysis, including 1D and 2D NMR, HR-ESI-MS, and chemical degradation method. Our results indicated that TKV inhibited lipid accumulation and triacylglycerol content occurred without cytotoxicity to 3T3-L1 adipocytes. Furthermore, TKV significantly suppressed the mRNA expression of nuclear transcription factors such as, peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α (C/EBPα). These results suggest that TKV shows the ability to inhibit 3T3-L1 preadipocyte differentiation of and may have therapeutic potential for obesity and its associated metabolic disorders.


Bupleurum chinense DC. Triterpene diglycoside Tibesaikosaponin V 



This work was financially supported by the West Light Foundation of Chinese Academy of Sciences (Y7C1031100), National Natural Science Foundation of China (21561142003, 31600281 and 41501262) Natural Science Foundation of Jiangsu Province (BK20171269), and Qing Lan Project of Jiangsu Province.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

44_2018_2279_MOESM1_ESM.docx (208 kb)
Supplementary Materials


  1. Chen R, Guo X, Cheng B, Gong Y, Ying B, Lin M (2018a) Saikosaponin a inhibits cigarette smoke-induced oxidant stress and inflammatory responses by activation of Nrf2. Inflammation 41:1297–1303CrossRefGoogle Scholar
  2. Chen X, Chen S, Liang W, Wang M, Li C, Wang S, Dong S, Yi L, Li C (2018b) Saikosaponin A attenuates perimenopausal depression-like symptoms by chronic unpredictable mild stress. Neurosci Lett 662:283–289CrossRefGoogle Scholar
  3. Choi KH, Lee HA, Park MH, Han J (2017) Cyanidin-3-rutinoside increases glucose uptake by activating the PI3K/Akt pathway in 3T3-L1 adipocytes. Environ Toxicol Pharmacol 54:1–6CrossRefGoogle Scholar
  4. Chyau C, Chu C, Chen S, Duh P (2018) The inhibitory effects of djulis (chenopodium formosanum) and its bioactive compounds on adipogenesis in 3T3-L1 adipocytes. Molecules 23:1780CrossRefGoogle Scholar
  5. Collins KH, Herzog W, MacDonald GZ, Reimer RA, Rios JL, Smith IC, Zernicke RF, Hart DA (2018) Obesity, metabolic syndrome, and musculoskeletal disease: common inflammatory pathways suggest a central role for loss of muscle integrity. Front Physiol 9:112CrossRefGoogle Scholar
  6. Committee of National Pharmacopoeia (2015) China pharmacopoeia, Vol. 1. Beijing, China, p 280Google Scholar
  7. Fang W, Yang Y, Guo B, Cen S (2017) Anti-influenza triterpenoid saponins (saikosaponins) from the roots of Bupleurum marginatum var. stenophyllum. Bioorg Med Chem Lett 27:1654–1659CrossRefGoogle Scholar
  8. Fasshauer M, Blüher M (2015) Adipokines in health and disease. Trends Pharmacol Sci 36:461–470CrossRefGoogle Scholar
  9. Guo J, Cao Y, Ho C, Jin S, Huang Q (2017) Aged citrus peel (chenpi) extract reduces lipogenesis in differentiating 3T3-L1 adipocytes. J Funct Foods 34:297–303CrossRefGoogle Scholar
  10. Jiang X, Wang L, Wang E, Zhang G, Chen B, Wang M, Li F (2018) Flavonoid glycosides and alkaloids from the embryos of Nelumbo nucifera seeds and their antioxidant activity. Fitoterapia 125:184–190CrossRefGoogle Scholar
  11. Kim E, Yi Y, Son Y, Han SY, Kim DH, Nam G, Hossain MA, Kim J, Park J, Cho JY (2018) BIOGF1K, a compound K-rich fraction of ginseng, plays an antiinflammatory role by targeting an activator protein-1 signaling pathway in RAW264.7 macrophage-like cells. J Ginseng Res 42:233–237CrossRefGoogle Scholar
  12. Kuang H, Sun S, Yang B, Xia Y, Feng W (2009) New megastigmane sesquiterpene and indole alkaloid glucosides from the aerial parts of Bupleurum chinense DC. Fitoterapia 80:35–38CrossRefGoogle Scholar
  13. Lee MS, Shin Y, Jung S, Kim SY, Jo YH, Kim CT, Yun MK, Lee SJ, Sohn J, Yu HJ, Kim Y (2017) The inhibitory effect of tartary buckwheat extracts on adipogenesis and inflammatory response. Molecules 22:1160CrossRefGoogle Scholar
  14. Li D, Wu J, Liu L, Wu Y, Li L, Huang X, Liu Q, Yang J, Song S, Wu C (2015) Cytotoxic triterpenoid glycosides (saikosaponins) from the roots of Bupleurum chinense. Bioorg Med Chem Lett 25:3887–3892CrossRefGoogle Scholar
  15. Li F, Yang F, Liu X, Wang L, Chen B, Li L, Wang M (2017a) Cucurbitane glycosides from the fruit of Siraitia grosvenori and their effects on glucose uptake in human HepG2 cells in vitro. Food Chem 228:567–573CrossRefGoogle Scholar
  16. Li H, Zhao Y, Zeng M, Fang F, Li M, Qin T, Ye L, Li H, Qu R, Ma S (2017b) Saikosaponin D relieves unpredictable chronic mild stress induced depressive-like behavior in rats: involvement of HPA axis and hippocampal neurogenesis. Psychopharmacology 234:3385–3394CrossRefGoogle Scholar
  17. Li Y, Cai T, Zhang W, Zhu W, Lv S (2017c) Effects of Saikosaponin D on apoptosis in human U87 glioblastoma cells. Mol Med Rep 16:1459–1464CrossRefGoogle Scholar
  18. Liang H, Zhao YY, Qiu HY, Huang J, Zhang RY (1998) A new saikosaponin from Bupleurum chinense DC. Acta Pharm Sin 33:37–41Google Scholar
  19. Liang H, Cui YJ, Zhao YY, Wang B, Yang WX, Yu Y (2001a) Saikosaponin v-2 from Bupleurum Chinense. Chin Chem Lett 12:331–332Google Scholar
  20. Liang H, Han ZY, Zhao YY, Wang B, Cui YX, Yang WX, Yu Y (2001b) Saikosaponin q-1 from Bupleurum chinense. Acta Bot Sin 43:198–200Google Scholar
  21. Liang ZT, Qin MJ, Wang ZT, Yu GD (2001c) The advance on the research of saponins of Bupleurum. Nat Prod Res Dev 13:67–72Google Scholar
  22. Liu QX, Liang H, Zhao YY, Wang B, Yang WX, Yu Y (2001) Saikosaponin v-1 from roots of Bupleurum chinense DC. J Asian Nat Prod Res 3:139–144CrossRefGoogle Scholar
  23. Lorrai I, Maccioni P, Carai MA, Capra A, Castelli MP, Riva A, Morazzoni P, Gessa GL, Colombo G (2017) Suppressing effect of saikosaponin A, an active ingredient of Bupleurum falcatum, on chocolate self-administration and reinstatement of chocolate seeking in rats. Neurosci Lett 638:211–217CrossRefGoogle Scholar
  24. Moseti D, Regassa A, Kim W (2016) Molecular regulation of adipogenesis and potential anti-adipogenic bioactive molecules. Int J Mol Sci 17:124CrossRefGoogle Scholar
  25. Nishina A, Itagaki M, Sato D, Kimura H, Hirai Y, Phay N, Makishima M (2017) The rosiglitazone-like effects of vitexilactone, a constituent from Vitex trifolia L. in 3T3-L1 preadipocytes. Molecules 22:2030CrossRefGoogle Scholar
  26. Ohashi K, Shibata R, Murohara T, Ouchi N (2014) Role of anti-inflammatory adipokines in obesity-related diseases. Trends Endocrinol Metab 25:348–355CrossRefGoogle Scholar
  27. Patel TP, Rawal K, Bagchi AK, Akolkar G, Bernardes N, Dias DD, Gupta S, Singal PK (2016) Insulin resistance: an additional risk factor in the pathogenesis of cardiovascular disease in type 2 diabetes. Heart Fail Rev 21:11–23CrossRefGoogle Scholar
  28. Rosen ED, MacDougald OA (2006) Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol 7:885–896CrossRefGoogle Scholar
  29. Tsuyoshi H, Wong VK, Han Y, Orisaka M, Yoshida Y, Tsang BK (2017) Saikosaponin-d, a calcium mobilizing agent, sensitizes chemoresistant ovarian cancer cells to cisplatin-induced apoptosis by facilitating mitochondrial fission and G2/M arrest. Oncotarget 8:99825CrossRefGoogle Scholar
  30. Wang HW, Liu M, Zhong TD, Fang XM (2015) Saikosaponin-d attenuates ventilator-induced lung injury in rats. Int J Clin Exp Med 8:15137–15145PubMedPubMedCentralGoogle Scholar
  31. Wang Y, Guo Q, Cheng Z, Zeng K, Liang H, Tu P, Chen S, Zhang Q (2017) New saikosaponins from the roots of Bupleurum chinense. Phytochem Lett 21:183–189CrossRefGoogle Scholar
  32. Yu J, Deng A, Wu L, Zhang Z, Liu Y, Wang W, Qin H (2013) Osteoclast-inhibiting saikosaponin derivatives from Bupleurum Chinense. Fitoterapia 85:101–108CrossRefGoogle Scholar
  33. Zhang T, Zhou J, Wang Q (2007) Flavonoids from aerial part of Bupleurum chinense DC. Biochem Syst Ecol 35:801–804CrossRefGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection/Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze LakeHuaiyin Normal UniversityHuaianChina
  2. 2.Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of BiologyChinese Academy of SciencesChengduChina
  3. 3.Department of ChemistryWomen University of Azad Jammu and KashmirBaghPakistan
  4. 4.Department of GerontologyThe Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityHuaianChina

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