Abstract
A novel RP-HPLC method for the determination of mefenamic acid in raw material, different pharmaceutical forms, and in human serum for routine analysis, therapeutic purposes, and stability studies was developed and validated. This study is also a collection of studies performed on the physical and chemical aspects of mefenamic acid. The method developed constitutes mobile phase, acetonitrile:acetic acid:water (72.5:1:26.5, v/v/v) at pH 3 and mefenamic acid was monitored with UV detection at 279 nm, eluting out at 3.98 min. The present HPLC method was found to be linear (100–300 μg mL−1), accurate, and least time consuming with very good recovery. The limits of detection of the method were found to be 10 μg mL−1. This method was also used to study the stability profile of mefenamic acid in tablets for 5 years and suspension for 4 years. The excipients present in the formulation did not interfere with the assay. The present method was also compared with the UV–visible technique and it was observed that HPLC method is more precise, sensitive, and accurate.
Similar content being viewed by others
References
Almasirad A, Tajik M, Bakhtiari D, Shafiee A, Abdollahi M, Zamani MJ, Khorasani R, Esmaily H (2005) Synthesis and analgesic activity of N-arylhydrazone derivatives of mefenamic acid. J Pharm Sci 8:419
Aly FA, Al-tamimi SA, Alwarthan AA (2000) Determination of flufenamic acid and mefenamic acid in pharmaceutical preparations and biological fluids using flow injection analysis with tris(2, 2′-bipyridyl)ruthenium(II) chemiluminescence detection. Anal Chim Acta 416:87
Anju G, Singhvi (2008) Spectrophotometric estimation of ethamsylate and mefenamic acid from a binary mixture by dual wavelength and simultaneous equation methods. Ind J Pharm Sci 70:108
Arayne MS, Sultana N, Siddiqui FA (2008) Simultaneous determination of pyridoxine, meclizine and buclizine in dosage formulations and human serum by RP-LC. Chromatographia 67:941–945
Chilukuri SP, Ramamohana A (1989) Spectrophotometric determination of some analgesic and anti-inflammatory agents with 3-methyl-2-benzothiazolinone hydrazone hydrochloride. J Mic Acta 97:237
Dahivelkar PP, Mahajan VK, Bari SB, Shirkhedkar AA, Fursule RA, Surana SJ (2007) Simultaneous derivative and multi-component spectrophotometric determination of drotaverine hydrochloride and mefenamic acid in tablets. Ind J Pharm Sci 69:812
Das S, Sharma SC, Talwar SK, Sethi PD (1989) Simultaneous spectrophotometric determination of mefenamic acid and paracetamol in pharmaceutical preparations. Analyst 114:101
Hładon T, Pawlaczyk J, Szafran B (1999) Stability of mefenamic acid in the inclusion complex with cyclodextrin in the solid phase. J Inclusion Phenom Macrocyclic Chem 35:497
ICH, Topic Q2(R1) (2005) Validation of analytical procedures: methodology. ICH, Geneva
Itai S, Nemoto M, Kouchiwa S, Murayama H, Nagai T (1985) Mefenamic acid is a high-dose nonsteroidal anti-inflammatory agent prepared as immediate-release capsule and tablet formulations. Chem Pharm Bull 33:5464
Luner PE, Babu SR, Mehta SC (1996) Wet ability of a hydrophobic drug by surfactant solutions. Int J Pharm 128:29
Mikami EI, Goto T, Ohno T, Matsumoto H, Inagaki K, Ishihara H, Nishida M (2000) Simultaneous analysis of anthranilic acid derivatives in pharmaceuticals and human urine by high-performance liquid chromatography with isocratic elution. J Chromatogr B 744:81
Mohammad RR, Ali A, Yalda HA, Fakhredin A (2004) Liquid chromatography method for determination of mefenamic acid in human serum. J Chromatogr B 800:189–192
Niopas I, Mamzoridi KJ (1994) Determination of indomethacin and mefenamic acid in plasma by high-performance liquid chromatography. Chromatogr B Biomed Appl 6:447
Shadab Q (1996) ICS and bioavailability of nonsteroidal antiinflammatory drugdisposition kinets,.mefenamic acid and piroxicam, in normal, metabolically altered and water deprived laboratory, PhD thesis, 262 p
Toral MI, Richter P, Araya E, Fuentes S (1996) Determination of mefenamic acid and paracetamol by first derivative spectrophotometry. Anal Lett 29:2679
United States Pharmacopeia/National Formulary (2007) United States pharmacopeial convention, 30th edn. Rockville, MD
Yogini J, Gokul T, Sanjay S (2007) Application of HPLC for the simultaneous determination of ethamsylate and mefenamic acid in bulk drugs and tablets. J Liq Chromat Related Technol 30:1115
Zhou W, Gilpin RK (2004) Rapid ESI-MS method for examining the thermal decomposition of pharmaceuticals. J Pharm Sci 93:1545–1556
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Shah, S.N., Mirza, A.Z., Shamshad, H. et al. Physical and chemical characterization of mefenamic acid in different pharmaceutical dosage forms and their stability studies using novel RP-HPLC method. Med Chem Res 21, 3591–3597 (2012). https://doi.org/10.1007/s00044-011-9897-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00044-011-9897-5