Aquatic Sciences

, 81:71 | Cite as

N2 and N2O production and emission variation during the flood period of Poyang Lake (China)

  • Jingya Xue
  • Xiaolong Yao
  • Zhonghua Zhao
  • Xingyu Jiang
  • Qiushi Shen
  • Yuwei Chen
  • Lu ZhangEmail author
Research Article


Lakes are globally important sites to alleviate nitrogen (N) concentrations through gaseous N production and emission, and hydro-geomorphological characteristics can influence the spatial variation, patterns, and efficiency of N removal in lakes. In this study, gaseous N removal via dinitrogen (N2) and nitrous oxide (N2O) production and emission in different hydro-geomorphological areas of Poyang Lake, China, were investigated through direct measurement of excess dissolved N2 and N2O (ΔN2 and ΔN2O) using the N2:Ar ratio method and headspace equilibrium technique, respectively. The highest value of ΔN2 (75.2 ± 31.0 μmol N2 L−1) was observed in the open lake, while the lowest ΔN2 (47.2 ± 14.9 μmol N2 L−1) occurred in the south estuarine delta (p < 0.05). However, the distribution of ΔN2O was opposite to that of ΔN2 with the highest ΔN2O being observed in the south estuarine delta (5.1 ± 5.0 nmol N2O L−1). Gaseous N2 removal fraction (the ΔN2 proportion among the sum of dissolved inorganic nitrogen (DIN) concentration and ΔN2) varied from 36% in the south estuarine delta to 48% in the open lake, while N2O yield (the ΔN2O proportion among the sum of ΔN2 and ΔN2O) varied from 0.04‰ in the open lake to 0.10‰ in the south estuarine delta. Correlation analyses showed that NO3 was the main factor controlling N2O yield. This study confirmed that N removal fractions and patterns vary with different hydro-geomorphological areas.


Gaseous nitrogen removal Dinitrogen Nitrous oxide Floodplain lake 



This study was financially supported by the National Natural Science Foundation of China (Nos. 41771519, 41671477 and 51839011), the Chinese Academy of Sciences Technology Service Network Program (STS) and Major Science and Technology Program for Water Pollution Control and Treatment (2017ZX07204005). We thank the Poyang Lake Laboratory for Wetland Ecosystem Research (PLLWER) for providing the instruments and labs. We sincerely thank Weitao Lin and Ting Liu for their assistance in field sampling and sample analyses. We also thank the anonymous reviewers for their careful review and suggestions.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

27_2019_668_MOESM1_ESM.docx (609 kb)
Supplementary material 1 (DOCX 609 kb)


  1. Beaulieu JJ, Tank JL, Hamilton SK, Wollheim WM Jr, Hall R, Mulholland PJ, Peterson BJ, Ashkenas LR, Cooper LW, Dahm CN (2011) Nitrous oxide emission from denitrification in stream and river networks. Proc Natl Acad Sci USA 108(1):214CrossRefGoogle Scholar
  2. Blackmer A, Bermner J (1978) Inhibitory effect of nitrate on reduction of N2O to N2 by soil microorganisms. Soil Biol Biochem 10:187–191CrossRefGoogle Scholar
  3. Canfield DE, Glazer AN, Falkowski PG (2010) The evolution and future of Earth’s nitrogen cycle. Science (N Y) 330:192–196CrossRefGoogle Scholar
  4. Chen N, Wu J, Chen Z, Lu T, Wang L (2014) Spatial-temporal variation of dissolved N2 and denitrification in an agricultural river network, southeast China. Agric Ecosyst Environ 189:1–10CrossRefGoogle Scholar
  5. Chen N, Wu J, Zhou X, Chen Z, Lu T (2015) Riverine N2O production, emissions and export from a region dominated by agriculture in Southeast Asia (Jiulong River). Agric Ecosyst Environ 208:37–47CrossRefGoogle Scholar
  6. Dalsgaard T, Canfield DE, Petersen J, Thamdrup B, Acuña-González J (2003) N2 production by the anammox reaction in the anoxic water column of Golfo Dulce, Costa Rica. Nature 422(6932):606–608CrossRefGoogle Scholar
  7. Davidson TA, Audet J, Svenning JC, Lauridsen TL, Sondergaard M, Landkildehus F, Larsen SE, Jeppesen E (2015) Eutrophication effects on greenhouse gas fluxes from shallow-lake mesocosms override those of climate warming. Glob Chang Biol 21(12):4449–4463CrossRefGoogle Scholar
  8. Dawson RN, Murphy KL (1972) The temperature dependency of biological denitrification. Water Res 6:71–83CrossRefGoogle Scholar
  9. Feng L, Hu C, Chen X, Li R, Tian L, Murch B (2011) MODIS observations of the bottom topography and its inter-annual variability of Poyang Lake. Remote Sens Environ 115(10):2729–2741CrossRefGoogle Scholar
  10. Feng L, Hu C, Chen X, Zhao X (2013) Dramatic inundation changes of China’s two largest freshwater lakes linked to the three Gorges Dam. Environ Sci Technol 47(17):9628–9634CrossRefGoogle Scholar
  11. Finlay JC, Small GE, Sterner RW (2013) Human influences on nitrogen removal in lakes. Science 342(6155):247–250CrossRefGoogle Scholar
  12. Galloway JN, Aber JD, Erisman JW, Seitzinger SP, Howarth RW, Cowling EB, Cosby BJ (2003) The Nitrogen cascade. Bioscience 53(4):341–356CrossRefGoogle Scholar
  13. Galloway JN, Townsend AR, Erisman JW, Bekunda M, Cai Z, Freney JR, Martinelli LA, Seitzinger SP, Sutton MA (2008) Transformation of the nitrogen cycle: recent trends, questions, and potential solutions. Science 320(5878):889–892CrossRefGoogle Scholar
  14. Gardner WS, McCarthy MJ (2009) Nitrogen dynamics at the sediment-water interface in shallow, sub-tropical Florida Bay: why denitrification efficiency may decrease with increased eutrophication. Biogeochemistry 95(2–3):185–198CrossRefGoogle Scholar
  15. Garnier J, Cébron A, Tallec G, Billen G, Sebilo M, Martinez A (2006) Nitrogen behaviour and nitrous oxide emission in the Tidal Seine River Estuary (France) as influenced by human activities in the upstream watershed. Biogeochemistry 77(3):305–326CrossRefGoogle Scholar
  16. Hansen AT, Dolph CL, Foufoula-Georgiou E, Finlay JC (2018) Contribution of wetlands to nitrate removal at the watershed scale. Nat Geosci 11(2):127–132CrossRefGoogle Scholar
  17. Huttunen JT (2003) Nitrous oxide flux to the atmosphere from the littoral zone of a boreal lake. J Geophys Res 108(D14):4421CrossRefGoogle Scholar
  18. Huttunen JT, Väisänen TS, Hellsten SK, Heikkinen M, Nykänen H, Jungner H, Niskanen A, Virtanen MO, Lindqvist OV, Nenonen OS (2002) Fluxes of CH4, CO2, and N2O in hydroelectric reservoirs Lokka and Porttipahta in the northern boreal zone in Finland. Glob Biogeochem Cycles 16(1):3-1–3-17CrossRefGoogle Scholar
  19. Jin X, Tu Q (1990) The stand methods for observation and analysis in Lake Eutrophication. Chinese Environmental Science Press, Beijing (in Chinese) Google Scholar
  20. Jordan SJ, Stoffer J, Nestlerode JA (2011) Wetlands as sinks for reactive nitrogen at continental and global scales: a meta-analysis. Ecosystems 14(1):144–155CrossRefGoogle Scholar
  21. Kuypers MMM, Sliekers AO, Lavik G, Schmid M, Jørgensen BB, Kuenen JG, Sinninghe Damsté JS, Marc S, Jetten MSM (2003) Anaerobic ammonium oxidation by anammox bacteria in the Black Sea. Nature 422(6932):608CrossRefGoogle Scholar
  22. Kuypers MMM, Marchant HK, Kartal B (2018) The microbial nitrogen-cycling network. Nat Rev Microbiol 16(5):263–276CrossRefGoogle Scholar
  23. Liu Y (2012) Climate hydrological process and environmental effects in Poyang Lake Basin. Science Press, Beijing (in Chinese) Google Scholar
  24. Liu W, Yao L, Wang Z, Xiong Z, Liu G (2015) Human land uses enhance sediment denitrification and N2O production in Yangtze lakes primarily by influencing lake water quality. Biogeosciences 12(20):6059–6070CrossRefGoogle Scholar
  25. Liu X, Beusen AHW, Van Beek LPH, Mogollon JM, Ran X, Bouwman AF (2018) Exploring spatiotemporal changes of the Yangtze River (Changjiang) nitrogen and phosphorus sources, retention and export to the East China Sea and Yellow Sea. Water Res 142:246–255CrossRefGoogle Scholar
  26. Marzadri A, Tonina D, Bellin A, Tank JL (2014) A hydrologic model demonstrates nitrous oxide emissions depend on streambed morphology. Geophys Res Lett 41(15):5484–5491CrossRefGoogle Scholar
  27. Mulholland PJ, Helton AM, Poole GC, Hall RO Jr, Hamilton SK, Peterson BJ (2008) Stream denitrification across biomes and its response to anthropogenic nitrate loading. Nature 452:202-U46CrossRefGoogle Scholar
  28. Nision T, Koike I, Hattori A (1983) Estimates of denitrification and nitrification in coastal and estuarine sediments. Appl Environ Microbiol 45(2):444–450Google Scholar
  29. Nizzoli D, Carraro E, Nigro V, Viaroli P (2010) Effect of organic enrichment and thermal regime on denitrification and dissimilatory nitrate reduction to ammonium (DNRA) in hypolimnetic sediments of two lowland lakes. Water Res 44(9):2715–2724CrossRefGoogle Scholar
  30. Pribyl AL, McCutchan JH, Lewis WM, Saunders JF (2005) Whole-system estimation of denitrification in a plains river: a comparison of two methods. Biogeochemistry 73:439–455CrossRefGoogle Scholar
  31. Ravishankara AR, Daniel JS, Portmann RW (2009) Nitrous oxide (N2O): the dominant ozone-depleting substance emitted in the 21st century. Science 326(5949):123–125CrossRefGoogle Scholar
  32. Raymond PA, Cole JJ (2001) Gas exchange in rivers and estuaries: choosing a gas transfer velocity. Estuaries 24(2):312–317CrossRefGoogle Scholar
  33. Roley SS, Tank JL, Stephen ML, Johnson LT, Beaulieu JJ, Witter JD (2012) Floodplain restoration enhances denitrification and reach-scale nitrogen removal in an agricultural stream. Ecol Appl 22(1):281–297CrossRefGoogle Scholar
  34. Schmadel NM, Harvey JW, Alexander RB, Schwarz GE, Moore RB, Eng K, Gomezvelez JD, Boyer EW, Scott D (2018) Thresholds of lake and reservoir connectivity in river networks control nitrogen removal. Nat Commun 9(1):2779CrossRefGoogle Scholar
  35. Schubert CJ, Durisch-Kaiser E, Wehrli B, Bo T, Lam P, Kuypers MMM (2006) Anaerobic ammonium oxidation in a tropical freshwater system (Lake Tanganyika). Environ Microbiol 8(10):1857–1863CrossRefGoogle Scholar
  36. Seitzinger SP (1994) Linkages between organic matter mineralization and denitrification in eight riparian wetlands. Biogeochemistry 25:19–39CrossRefGoogle Scholar
  37. Shankman D, Keim BD, Song J (2006) Flood frequency in China’s Poyang Lake region: trends and teleconnections. Int J Climatol 26(9):1255–1266CrossRefGoogle Scholar
  38. Smith VH, Tilman GD, Nekola JC (1999) Eutrophication: impacts of excess nutrient inputs on freshwater, marine, and terrestrial ecosystems. Environ Pollut 100(1–3):179–196CrossRefGoogle Scholar
  39. Tang C, Zhang L, Du Y, Yao X (2014) Spatial variations of denitrification in wetland sediments in Poyang Lake and the influencing factors. Acta Sci Circum 34(1):202–209 (In Chinese) Google Scholar
  40. Wang S, Liu C, Yeager KM, Wan G, Li J, Tao F, Lu Y, Liu F, Fan C (2009) The spatial distribution and emission of nitrous oxide (N2O) in a large eutrophic lake in eastern China: anthropogenic effects. Sci Total Environ 407(10):3330–3337CrossRefGoogle Scholar
  41. Wang Y, Yu X, Li W, Xu J, Chen Y, Fan N (2011) Potential influence of water level changes on energy flows in a lake food web. Chin Sci Bull 56(26):2794–2802CrossRefGoogle Scholar
  42. Wang J, Chen N, Yan W, Wang B, Yang L (2015) Effect of dissolved oxygen and nitrogen on emission of N2O from rivers in China. Atmos Environ 103:347–356CrossRefGoogle Scholar
  43. Wanninkhof R (2014) Relationship between wind speed and gas exchange over the ocean revisited. Limnol Oceanogr Methods 12(6):351–362CrossRefGoogle Scholar
  44. Weiss RF (1970) The solubility of nitrogen, oxygen and argon in water and seawater. Deep-Sea Research and Oceanographic Abstracts 17(4):721–735CrossRefGoogle Scholar
  45. Weiss RF, Price BA (1980) Nitrous oxide solubility in water and seawater. Mar Chem 8(4):347–359CrossRefGoogle Scholar
  46. Wertz S, Goyer C, Zebarth BJ, Burton DL, Tatti E, Chantigny MH, Filion M (2013) Effects of temperatures near the freezing point on N2O emissions, denitrification and on the abundance and structure of nitrifying and denitrifying soil communities. FEMS Microbiol Ecol 83(1):242–254CrossRefGoogle Scholar
  47. Wu Z, Cai Y, Liu X, Xu CP, Chen Y, Zhang L (2013) Temporal and spatial variability of phytoplankton in Lake Poyang: the largest freshwater lake in China. J Great Lakes Res 39(3):476–483CrossRefGoogle Scholar
  48. Yan W, Yang L, Wang F, Wang J, Ma P (2012) Riverine N2O concentrations, exports to estuary and emissions to atmosphere from the Changjiang River in response to increasing nitrogen loads. Glob Biogeochem Cycles 26(4):1–15CrossRefGoogle Scholar
  49. Zhang L, Chen X, Zhang Y, Chen L, Zhang P (2014a) Spatial distribution of water quality and its impacting factor in the wet season of Poyang Lake using the hydro-geomorphological partitions. China Environtal Science 34(10):2637–2645 (In Chinese) Google Scholar
  50. Zhang Q, Ye X, Werner AD, Li Y, Yao J, Li X, Xu C (2014b) An investigation of enhanced recessions in Poyang Lake: comparison of Yangtze River and local catchment impacts. J Hydrol 517:425–434CrossRefGoogle Scholar
  51. Zhang L, Yao X, Tang C, Xu H, Jiang X, Zhang Y (2016) Influence of long-term inundation and nutrient addition on denitrification in sandy wetland sediments from Poyang Lake, a large shallow subtropical lake in China. Environ Pollut 219:440–449CrossRefGoogle Scholar
  52. Zhao Y, Xia Y, Kana TM, Wu Y, Li X, Yan X (2013) Seasonal variation and controlling factors of anaerobic ammonium oxidation in freshwater river sediments in the Taihu Lake region of China. Chemosphere 93(9):2124–2131CrossRefGoogle Scholar
  53. Zhu G, Wang S, Wang W, Wang Y, Zhou L, Jiang B, Camp H, Risgaardpetersen N, Schwark L, Peng Y (2013) Hotspots of anaerobic ammonium oxidation at land-freshwater interfaces. Nat Geosci 6(2):103–107CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Jingya Xue
    • 1
    • 2
  • Xiaolong Yao
    • 1
    • 2
  • Zhonghua Zhao
    • 1
  • Xingyu Jiang
    • 1
    • 2
  • Qiushi Shen
    • 1
  • Yuwei Chen
    • 1
  • Lu Zhang
    • 1
    Email author
  1. 1.State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and LimnologyChinese Academy of SciencesNanjingChina
  2. 2.University of Chinese Academy of ScienceBeijingChina

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