Cellular and Molecular Life Sciences

, Volume 76, Issue 24, pp 4861–4867 | Cite as

Programmable RNA manipulation in living cells

  • Yu Pei
  • Mingxing LuEmail author


RNAs are responsible for mediating genetic information flow within the cell. RNA splicing, modification, trafficking, translation, and stability are all controlled at the transcript level. However, biological tools to study and manipulate them in a programmable fashion are currently limited. In this review, we summarize recent advances regarding available RNA-targeting systems discovered so far, including CRISPR-based technologies—Cas9 and Cas13, and programmable RNA-binding proteins—PUF and PPR. These tools allow transcript-specific manipulation in gene expression.


CRISPR RNA-binding proteins Cas9 Cas13 PUF PPR APEX2 bioID 



Dr. Jianling Xie for comments.

Author contributions

MXL conceived the ideas and wrote the manuscript together with YP.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.


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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Physiology and PharmacologyKarolinska InstitutetStockholmSweden
  2. 2.Department of Biomedical Sciences, College of Veterinary Medicine and Life SciencesCity University of Hong KongKowloon TongHong Kong

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