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Cellular and Molecular Life Sciences

, Volume 76, Issue 19, pp 3745–3752 | Cite as

Modulation of miRNA function by natural and synthetic RNA-binding proteins in cancer

  • Pascal D. Vos
  • Peter J. Leedman
  • Aleksandra Filipovska
  • Oliver RackhamEmail author
Review

Abstract

RNA-binding proteins (RBPs) and microRNAs (miRNAs) are the most important regulators of mRNA stability and translation in eukaryotic cells; however, the complex interplay between these systems is only now coming to light. RBPs and miRNAs regulate a unique set of targets in either a positive or negative manner and their regulation is mainly opposed to each other on overlapping targets. In some cases, the levels of RBPs or miRNAs regulate the cellular levels of one another and decreased levels of either results in changes in translation of their targets. There is growing evidence that these regulatory circuits are crucial in the development and progression of cancer; however, the rules underlying synergism and antagonism between miRNAs and RNA-binding proteins remain unclear. Synthetic biology seeks to develop artificial systems to better understand their natural counterparts and to develop new, useful technologies for manipulation of gene expression at the RNA level. The recent development of artificial RNA-binding proteins promises to enable a much greater understanding of the importance of the functional interactions between RNA-binding proteins and miRNAs, as well as enabling their manipulation for therapeutic purposes.

Keywords

miRNA Designer RNA-binding proteins Pentatricopeptide repeat PUF domain Synthetic biology RNA–protein interactions RNA interference 

Notes

Acknowledgements

Research in our groups has been supported by fellowships, scholarships and grants from the Australian Research Council (FT0991008, FT0991113, DP140104111 to A. F. and O. R.), the National Health and Medical Research Council (APP1058442, APP1045677 to A. F. and O. R.,APP1071081 and APP1084964 to P. L), and the Cancer Council Western Australia (to A. F. and O. R.).

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Harry Perkins Institute of Medical Research, QEII Medical CentreNedlandsAustralia
  2. 2.Centre for Medical ResearchThe University of Western AustraliaNedlandsAustralia
  3. 3.School of Molecular and Chemical SciencesThe University of Western AustraliaCrawleyAustralia
  4. 4.Medical SchoolThe University of Western AustraliaCrawleyAustralia
  5. 5.School of Pharmacy and Biomedical SciencesCurtin UniversityBentleyAustralia
  6. 6.Curtin Health Innovation Research Institute, Curtin UniversityBentleyAustralia

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