The deubiquitinase OTUD5 regulates Ku80 stability and non-homologous end joining
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The ability of cells to repair DNA double-strand breaks (DSBs) is important for maintaining genome stability and eliminating oncogenic DNA lesions. Two distinct and complementary pathways, non-homologous end joining (NHEJ) and homologous recombination (HR), are employed by mammalian cells to repair DNA DSBs. Each pathway is tightly controlled in response to increased DSBs. The Ku heterodimer has been shown to play a regulatory role in NHEJ repair. Ku80 ubiquitination contributes to the selection of a DSB repair pathway by causing the removal of Ku heterodimers from DSB sites. However, whether Ku80 deubiquitination also plays a role in regulating DSB repair is unknown. To address this question, we performed a comprehensive study of the deubiquitinase specific for Ku80, and our study showed that the deubiquitinase OTUD5 serves as an important regulator of NHEJ repair by increasing the stability of Ku80. Further studies revealed that OTUD5 depletion impaired NHEJ repair, and hence reduced overall DSB repair. Furthermore, OTUD5-depleted cells displayed excess end resection; as a result, HR repair was facilitated by OTUD5 depletion during the S/G2 phase. In summary, our study demonstrates that OTUD5 is a specific deubiquitinase for Ku80 and establishes OTUD5 as an important and positive regulator of NHEJ repair.
KeywordsXRCC5 DUBA DNA lesion Deubiquitinases library DNA damage response
We are grateful to Dr. Jiadong Wang for his suggestion and help. We thank Qihua He for her suggestion and help, and Center of Medical and Health Analysis, Peking University, for confocal microscopy. We appreciate the ALENABIO (Xi’an, China) Company (http://www.alenabio.com) for the pathological micro-tissues (Cat. No. BC03119a). We deeply appreciate help from Ning Kon for editing our manuscript.
FL and WZ, conceived and designed the study that led to the submission, acquired data, and interpreted the results; FL performed the experiment; QS, KL, HH, QH, ZC, YM, and FT participated in the revision of the manuscript; ZM and TT approved the final version; and WZ was the corresponding author. The authors declare that they have no conflicts of interest associated with the contents of this article.
Wenhui Zhao was supported by the National Natural Science Foundation of China (Grant No. 85141044).
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Conflict of interest
The authors declare that they have no competing interests.
Availability of data and material
All of the data and material in this paper are available upon request.
- 4.Lieber MR (2010) The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathway. Annu Rev Biochem 79:181–211. https://doi.org/10.1146/annurev.biochem.052308.093131 CrossRefGoogle Scholar
- 6.San Filippo J, Sung P, Klein H (2008) Mechanism of eukaryotic homologous recombination. Annu Rev Biochem 77:229–257. https://doi.org/10.1146/annurev.biochem.77.061306.125255 CrossRefGoogle Scholar
- 25.Symington LS, Gautier J (2011) Double-strand break end resection and repair pathway choice. Annu Rev Genet 45:247–271. https://doi.org/10.1146/annurev-genet-110410-132435 CrossRefGoogle Scholar