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Cellular and Molecular Life Sciences

, Volume 72, Issue 12, pp 2415–2429 | Cite as

Protein composition and movements of membrane swellings associated with primary cilia

  • Ashraf M. Mohieldin
  • Hanan S. Haymour
  • Shao T. Lo
  • Wissam A. AbouAlaiwi
  • Kimberly F. Atkinson
  • Christopher J. Ward
  • Min Gao
  • Oliver Wessely
  • Surya M. NauliEmail author
Research Article

Abstract

Dysfunction of many ciliary proteins has been linked to a list of diseases, from cystic kidney to obesity and from hypertension to mental retardation. We previously proposed that primary cilia are unique communication organelles that function as microsensory compartments that house mechanosensory molecules. Here we report that primary cilia exhibit membrane swellings or ciliary bulbs, which based on their unique ultrastructure and motility, could be mechanically regulated by fluid-shear stress. Together with the ultrastructure analysis of the swelling, which contains monosialodihexosylganglioside (GM3), our results show that ciliary bulb has a distinctive set of functional proteins, including GM3 synthase (GM3S), bicaudal-c1 (Bicc1), and polycystin-2 (PC2). In fact, results from our cilia isolation demonstrated for the first time that GM3S and Bicc1 are members of the primary cilia proteins. Although these proteins are not required for ciliary membrane swelling formation under static condition, fluid-shear stress induced swelling formation is partially modulated by GM3S. We therefore propose that the ciliary bulb exhibits a sensory function within the mechano-ciliary structure. Overall, our studies provided an important step towards understanding the ciliary bulb function and structure.

Keywords

Primary cilia Ciliary bulb Ciliary membrane swelling Fluid-shear stress Bicc-1 GM3S PC2 

Notes

Acknowledgments

This work partially fulfills the requirements of a PhD degree in Medicinal and Biological Chemistry for Ashraf M. Mohieldin. This work was supported by the Department of Defense PR130153 (SMN) and in part by the National Institute of Health R01DK080640 (SMN), R01DK080745 (OW) and F31DK096870 (AMM).

Supplementary material

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Copyright information

© Springer Basel 2015

Authors and Affiliations

  • Ashraf M. Mohieldin
    • 1
    • 2
  • Hanan S. Haymour
    • 2
  • Shao T. Lo
    • 2
  • Wissam A. AbouAlaiwi
    • 2
  • Kimberly F. Atkinson
    • 3
  • Christopher J. Ward
    • 4
  • Min Gao
    • 5
  • Oliver Wessely
    • 6
  • Surya M. Nauli
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of Medicinal and Biological ChemistryUniversity of ToledoToledoUSA
  2. 2.Department of Pharmacology and Experimental TherapeuticsUniversity of ToledoToledoUSA
  3. 3.Department of Biomedical and Pharmaceutical SciencesChapman UniversityIrvineUSA
  4. 4.Department of Medicine, The Kidney InstituteUniversity of Kansas Medical CenterKansasUSA
  5. 5.Liquid Crystal InstituteKent State UniversityKentUSA
  6. 6.Department of Cellular and Molecular Medicine, Lerner Research InstituteCleveland Clinic FoundationClevelandUSA

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