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MiR-802 alleviates lipopolysaccharide-induced acute lung injury by targeting Peli2

  • Qinghai YouEmail author
  • Jinmei Wang
  • Dan Jia
  • Lijuan Jiang
  • Yuanmin Chang
  • Wenmei Li
Original Research Paper
  • 28 Downloads

Abstract

Introduction

Acute respiratory distress syndrome (ARDS) is a life-threatening medical condition. It is characterized by serious lung inflammation or injury. Characterizing novel miRNAs implicated in ARDS pathogenesis may provide new therapeutic strategy for managing ARDS.

Methods

We employed LPS-induced lung injury model to profile miRNAs associated with ARDS. We isolated one miRNA candidate and characterized its role in lipopolysaccharide (LPS)-induced proinflammatory cytokine production in lung macrophages. We further evaluated its functional role in ARDS model by assessing histological change, neutrophil activation, tissue permeability and tumor necrosis factor alpha (TNFα) production. We also characterized its downstream target using luciferase assay, Western blotting, enzyme-linked immunosorbent assay and cell inflammation assay.

Results

Microarray profiling revealed miR-802 was significantly downregulated in ARDS mouse model. LPS-induced miR-802 downregulation was confirmed in lung macrophages. Overexpression of miR-802 significantly suppressed LPS-induced inflammatory cytokine production in vitro and alleviates LPS-induced acute lung injury in vivo. Peli2 was identified as a downstream target of miR-802 and found upregulated in ARDS model. Overexpressing Peli2 abolished the antagonizing effect of miR-802 on LPS-mediated inflammatory response.

Conclusion

MiR-802 carried a protective role against LPS-induced acute lung injury by downregulating Peli2. MiR-802/Peli2 axis may act as intervening targets to manage ARDS.

Keywords

Acute respiratory distress syndrome miR802 Sepsis Peli2 Lung injury 

Notes

Funding

The study was supported by the National Natural Science Foundation of China (81100053).

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.

Ethical approval

The animal study was carried out according to the ethical guidelines approved by Animal Care and Use Committee in the First Affiliated Hospital of Anhui medical University.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Qinghai You
    • 1
    Email author
  • Jinmei Wang
    • 2
  • Dan Jia
    • 3
  • Lijuan Jiang
    • 4
  • Yuanmin Chang
    • 5
  • Wenmei Li
    • 5
  1. 1.Department of Respiratory and Critical Care MedicineThe First Affiliated Hospital of Anhui Medical UniversityHefeiChina
  2. 2.Department of Respiratory MedicineThe Affiliated Hospital of Jining Medical CollegeJiningChina
  3. 3.Department of Respiratory MedicineThe First Affiliated Hospital of Huzhou Teachers CollegeHuzhouChina
  4. 4.Department of Respiratory MedicineThe Chest Hospital of Anhui ProvinceHefeiChina
  5. 5.Department of Respiratory and Critical Care MedicineThe First Affiliated Hospital of Anhui Medical UniversityHefeiChina

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