LncRNA MEG3 impacts proliferation, invasion, and migration of ovarian cancer cells through regulating PTEN
Objective and design
We investigated the expressions of lncRNA MEG3 and PTEN in ovarian cancer tissues and their effects on cell proliferation, cycle and apoptosis of ovarian cancer.
Expression levels of MEG3 in ovarian cancer cell lines and normal ovarian cell lines were detected by qRT-PCR. Cell viability was detected by MTT assay. Cell apoptosis and cell cycle distribution were measured by flow cytometry. Cell invasion capability was tested by transwell assay. Cell migration capacity was tested by wound healing. The xenograft model was constructed to explore the effect of lncRNA MEG3 on ovarian cancer in vivo.
Compared with normal ovarian cells, expression levels of MEG3 and PTEN were relatively lower in ovarian cancer cells. There was a positive correlation between the expression of PTEN and the expression of MEG3. Enhanced expression level of PTEN suppressed SKOV3 cell proliferation, increased cell apoptosis rate, and decreased cell invasion and migration.
LncRNA MEG3 and PTEN were down-regulated in ovarian cancer cells. LncRNA MEG3 regulated the downstream gene PTEN in ovarian cancer cells to prohibit cell proliferation, promote apoptosis and block cell cycle progression.
KeywordsOvarian cancer LncRNA MEG3 PTEN
Analysis of variance
Long non-coding RNAs
Maternally expressed 3 gene
JW and WX: critical revision of the manuscript; YH and QX: substantial contribution to the conception and design of the work, and manuscript drafting; QX and YH: acquisition, analysis, and interpretation of the data; SL: revising the manuscript critically and final approval of the version to be published. All authors have read and approved the final article.
Compliance with ethical standards
Conflict of interest
The authors confirm that there are no conflicts of interest.
All procedures performed in studies involving animals were in accordance with the ethical standards of Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.