The Journal of Physiological Sciences

, Volume 65, Supplement 2, pp S11–S16 | Cite as

Low gamma wave oscillations in the striatum of mice following morphine administration

  • Chayaporn Reakkamnuan
  • Siriphun Hiranyachattada
  • Ekkasit KumarnsitEmail author
Original Paper


Functional role of the striatum in motor control has been widely studied. In addition, its involvement in reward function as a brain area in the dopamine system has also been mentioned. However, neural signaling in the striatum in response to consumption of emotional enhancing substances remained to be explored. This study aimed to investigate local field potential (LFP) of the striatum following morphine administration. Male Swiss albino mice implanted with electrode into the striatum were given an intraperitoneal injection of either saline or morphine (5 or 15 mg/kg). LFP and locomotor activity of individual animals were simultaneously recorded in the recording chamber following the administration. The inspection of LFP tracings revealed the increase in fast wave induced by morphine particularly at a high dose. Statistical analyses were performed using a one way ANOVA followed by Tukey post hoc test. Frequency analysis using Fast Fourier transform also confirmed a significant elevation of low gamma (30-44.9 Hz) activity. When analyzed in time domain, significant increase in low gamma power was observed from the 15th to 65th min following 15 mg/kg morphine treatment. Moreover, morphine treatment also exhibited a stimulating effect on locomotor speed. However, regression analyses revealed no significant correlation between low gamma power and locomotor speed. In summary, this study demonstrated the increase in low gamma oscillation in the striatum and this effect was not associated with locomotor activity of animals. Thus, it is possible that low gamma oscillation induced by morphine treatment is related with the reward function.


Striatum Local field potential Low gamma wave Morphine 


  1. 1.
    Volkow ND, Wang GJ, Fowler JS, Tomasi D, Telang F (2011) Addiction: Beyond dopamine reward circuitry. PNAS 108(37): 15037–15042.CrossRefGoogle Scholar
  2. 2.
    Olds J, Milner P (1954) Positive reinforcement produced by electrical stimulation of septal area and other regions of rat brain. J Comp Physiol Psychol 47(6): 419–427.CrossRefGoogle Scholar
  3. 3.
    Spanagel R, Weiss F (1999) The dopamine hypothesis of reward: past and current status. Trends Neurosci 22(11): 521–527.CrossRefGoogle Scholar
  4. 4.
    Wise RA (2009) Roles for nigrostriatal — not just mesocorticolimbic — dopamine in reward and addiction. Trends Neurosci 32(10): 517–524.CrossRefGoogle Scholar
  5. 5.
    Wang H-L, Morales M (2008) The corticotropin releasing factor binding protein (CRF-BP) within the ventral tegmental area is expressed in a subset of dopaminergic neurons. J Comp Neurol 509(3): 302–318.CrossRefGoogle Scholar
  6. 6.
    Fallon JH, Loughlin SE (1995) Substantia nigra. In: Paxinos G (ed) The Rat Nervous System, 2nd edn. Academic Press, New York.Google Scholar
  7. 7.
    Crow TJ (1972) A map of the rat mesencephalon for electrical self-stimulation. Brain Res 36(2): 265–273.CrossRefGoogle Scholar
  8. 8.
    Wise RA (1981) Intracranial self-stimulation: mapping against the lateral boundaries of the dopaminergic cells of the substantia nigra. Brain Res 213: 190–194.CrossRefGoogle Scholar
  9. 9.
    Delgado MR (2007) Reward-related responses in the human striatum. Ann N Y Acad Sci 1104: 70–88.CrossRefGoogle Scholar
  10. 10.
    Cheaha D, Bumrungsri S, Chatpun S, Kumarnsit E (2015) Characterization of in utero valproic acid mouse model of autism by local field potential in the hippocampus and the olfactory bulb. Neurosci Res 98: 28–34.CrossRefGoogle Scholar
  11. 11.
    Hnasko TS, Sotak BN, Palmiter RD (2005) Morphine reward in dopamine-deficient mice. Nature 438: 854–857.CrossRefGoogle Scholar
  12. 12.
    Gysling K, Wang RY (1983) Morphine-induced activation of A10 dopamine neurons in the rat. Brain research 277(1): 119–127.CrossRefGoogle Scholar
  13. 13.
    Chiara GD, Imperato A (1988) Drugs abused by humans preferentially increase synaptic dopamine concentrations in the mesolimbic system of freely moving rats. Proc Natl Acad Sci USA 85(14): 5274–5278.CrossRefGoogle Scholar
  14. 14.
    Johnson SW, North R (1992) Opioids excite dopamine neurons by hyperpolarization of local interneurons. J Neurosci 12(2): 483–488.CrossRefGoogle Scholar
  15. 15.
    Albin RL, Young AB, Penney JB (1989) The functional anatomy of basal ganglia disorders. Trends Neurosci 12(10): 366–375.CrossRefGoogle Scholar
  16. 16.
    Lotharius J, Brundin P (2002) Pathogenesis of Parkinson’s disease: dopamine, vesicles and α- synuclein. Nat Rev Neurosci 3: 932–942.CrossRefGoogle Scholar
  17. 17.
    Schultz W (1998) Predictive reward signal of dopamine neurons. J Neurophysiol 80(1): 1–27.CrossRefGoogle Scholar
  18. 18.
    Sun N, Li Y, Tian S, Lei Y, Zheng J, Yang J, Sui N, Xu L, Pei G, Wilson FAW, Ma Y, Lei H, Hu X (2006) Dynamic changes in orbitofrontal neuronal activity in rats during opiate administration and withdrawal. Neuroscience 138: 77–82.CrossRefGoogle Scholar
  19. 19.
    Kalenscher T, Lansink CS, Lankelma JV, Pennartz CMA (2010) Reward-associated gamma oscillation in ventral striatum are regionally differentiated and modulate local firing activity. J neurophysiol 103(3): 1658–1672.CrossRefGoogle Scholar
  20. 20.
    Masimore B, Schmitzer-Torbert NC, Kakalios J, Redish AD (2005) Transient gamma local field potential signal movement initiation in rats. Neuroreport 16(18): 2021–2024.CrossRefGoogle Scholar
  21. 21.
    Suto N, Wise RA, Vezina P (2001) Dorsal as well as ventral striatal lesions affect levels of intravenous cocaine and morphine self-administration in rats. Neurosci Lett 493(1-2): 29–32.CrossRefGoogle Scholar
  22. 22.
    Volkow ND, Wang GJ, Telang F, Fowler JS, Logan J, Childress AR, Jayne M, Ma Y, Wong C (2006) Cocaine cues and dopamine in dorsal striatum: Mechanism of craving in cocaine addiction. J Neurosci 26(24): 6583–6588.CrossRefGoogle Scholar
  23. 23.
    Herrmann CS, Munk MHJ, Engel AK (2004) Cognitive functions of gamma-band activity: memory match and utilization. Trends in cognitive sciences 8(8): 347–355.CrossRefGoogle Scholar
  24. 24.
    Masuda N (2009) Selective population rate coding: a possible computational role of gamma oscillations in selective attention. Neural Comput 21(12): 3335–3362.CrossRefGoogle Scholar
  25. 25.
    Tran AH, Tamura R, Uwana T, Kobayashi T, Katsuki M, Ono T (2005) Dopamine D1 receptors involved in locomotor activity and accumben neural responses to prediction of reward associated with place. PNAS 102(6): 2117–2122.CrossRefGoogle Scholar
  26. 26.
    Tran AH, Tamura R, Uwana T, Kobayashi T, Katsuki M, Matsumoto G, Ono T (2002) Altered accumbens neural response to prediction of reward associated with place in dopamine D2 receptor knockout mice. PNAS 99(13): 8986–8991.CrossRefGoogle Scholar

Copyright information

© The Physiological Society of Japan and Springer Japan 2015

Authors and Affiliations

  • Chayaporn Reakkamnuan
    • 1
  • Siriphun Hiranyachattada
    • 1
  • Ekkasit Kumarnsit
    • 1
    Email author
  1. 1.Department of Physiology, Faculty of SciencePrince of Songkla University (PSU)Hat Yai, SongkhlaThailand

Personalised recommendations