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Interleukin-10 and Interleukin-1 receptor antagonists increase during cardiac surgery

Abstract

Background

It has been reported that inflammatory cytokines such as interleukin-8 and 6 (IL-8, IL-6) increase during cardiac surgery and cause postoperative cardiac dysfunction. Therefore, it is important to investigate changes of suppressive cytokines such as IL-10, interleukin-4 (IL-4) and interleukin-1 receptor antagonist (IL-1 ra) dunng cardiac surgery.

Method

Serum levels of cytokines and IL-1 ra were measured in 10 patients during cardiac surgery with cardiopulmonary bypass. Six blood samples were drawn after inducing anaesthesia. In each sample, serum IL-10, IL-4, IL-8, IL-6 and IL-1 ra were measured by enzyme linked immunosorbent assay.

Results

Serum IL-6 and IL-8 concentration (19.1 ±8.8 pg · ml−1, and 13.4±5.2 pg · ml−1, preoperatively) increased to 227.5± 191 pg · ml−1 and 81.0±56 pg · ml−1 at 60 min after declamping the aorta (P< 0.01, respectively). Serum IL-10 concentration increased at 60 min after dedamping the aorta compared with the preoperative value (from 1.0±0 pg · ml−1 to 552.0± 158 pg · ml−1 P< 0.001]). Similarly, serum IL-1 ra concentration increased from the preoperative value of 1331±896 pg · ml−1 to 43353±12812 pg · ml−1 at 60 min after dedamping the aorta (P< 0.00l). Positive correlations were obtained between IL-10 and IL-8. and between IL-10 and IL-6 (γ=0.7, γ=0.8, P< 0.001, respectively).

Conclusion

These findings demonstrate that pro-and anti-inflammatory cytokines increase to maintain their balance during cardiac surgery.

Résumé

Objectif

On a rapporté que la concentration des cytokines de l’inflammation comme les interleukines 6 et 8 (IL-8. IL-6) s’élevaient pendant la chirurgie cardiaque et provoquaient des dérangements cardiaques postopératoires. II est donc aussi important d’examiner les perturbations produites par les cytokines suppressives comme IL-10, interleukine-4 (IL-4) et de l’antagoniste du récepteur de l’interleukine-1 (IL-1 ra) pendant la chirurgie cardiaque.

Méthodes

La concentration sérique des cytokines et de IL-1 ra a été mesurée chez dix patients pendant une chirurgie cardiaque sous CEC. Six échantillons de sang ont été prélevés après l’induction de l’anesthésie. Dans chacun des échantillons. on a titré IL-10, IL-4, IL-8, IL-6 et IL-1 ra avec l’épreuve de l’immuno-absorption enzymatique.

Résultats

Les concentrations de IL-6 et de IL-8 (valeurs préopératoires : 19, 1 ±8, 8 pg · ml−1 et 13.4±5.2 pg · ml−1) ont augmenté à 227,45±191 pg · ml−1 et 81, 0±56 pg · ml−1 60 min après le dédampage de l’aorte (respectivement P< 0, 01 ). La concentration sérique de IL-10 a augmenté 60 min après le dédampage de l’aorte comparativement aux valeurs préopératoires (de 1.0±0 pg · ml−1 à 552± 158 pg · ml−1, P < 0.001). De la même façon, la concentration sérique de IL-1 ra a augmenté de la valeur préopératoire de 1331 ±896 pg · ml−1 à 4 3353± 1 2812 pg · ml−1 60 min après le dédampage (P < 0,001). La corrélation était positive entre IL-10 et IL-8 et entre IL-10 et IL-6 (respectivement γ=0.7, γ=0.8, P < 0,001).

Conclusion

Ces données montrent que les cytokines pro- et anti-inflammatoires augmentent pour maintenir leur équilibre pendant la chirurgie cardiaque.

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Correspondence to Takae Kawamura.

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Kawamura, T., Wakusawa, R. & Inada, K. Interleukin-10 and Interleukin-1 receptor antagonists increase during cardiac surgery. Can J Anesth 44, 38–42 (1997). https://doi.org/10.1007/BF03014322

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Keywords

  • Cardiopulmonary Bypass
  • Aortic Occlusion
  • Human Alveolar Macrophage
  • Suppressive Cytokine
  • Inhibit Cytokine Production